Title:
GROWTH METHOD FOR NATURAL KILLER CELLS
Document Type and Number:
WIPO Patent Application WO/2010/013947
Kind Code:
A3
Abstract:
The present invention relates to an improved growth method for natural killer cells (NK cells). More specifically, the present invention relates to a growth method for natural killer cells, which comprises a step of culturing natural killer cells in a medium containing anti-CD3 antibodies and interleukin proteins in the presence of peripheral blood leukocytes. The present invention provides an innovative growth method for natural killer cells, which can obtain a large amount of natural killer cells by remarkably increasing the growth rate compared with conventional growth methods for natural killer cells.
Inventors:
AHN YONG OON (KR)
JUNG MI YOUNG (KR)
HEO DAE SEOG (KR)
HWANG YU KYEONG (KR)
JUNG MI YOUNG (KR)
HEO DAE SEOG (KR)
HWANG YU KYEONG (KR)
Application Number:
PCT/KR2009/004228
Publication Date:
May 27, 2010
Filing Date:
July 29, 2009
Export Citation:
Assignee:
GREEN CROSS CORP (KR)
SEOUL NAT UNIV HOSPITAL (KR)
AHN YONG OON (KR)
JUNG MI YOUNG (KR)
HEO DAE SEOG (KR)
HWANG YU KYEONG (KR)
SEOUL NAT UNIV HOSPITAL (KR)
AHN YONG OON (KR)
JUNG MI YOUNG (KR)
HEO DAE SEOG (KR)
HWANG YU KYEONG (KR)
International Classes:
C12N5/0783
Foreign References:
| US20080063717A1 | 2008-03-13 |
Other References:
OCHOA, A.C. ET AL.: "Long-term growth of lymphokine-activated killer (LAK) cells: Role of anti-CD3, p-IL 1, interferon-y AND -(beta.", JOURNAL OF IMMUNOLOGY, vol. 138, no. 8, 1987, pages 2728 - 2733
CARLENS, S. ET AL.: "A New Method for In Vitro Expansion of Cytotoxic Human CD3- CD56+ Natural Killer Cells.", HUMAN IMMUNOLOGY, vol. 62, 2006, pages 1092 - 1098
IGARASHI, T. ET AL.: "Enhanced cytotoxicity of allogeneic NK cells with killer immunoglobulin-like receptor ligand incompatibility against melanoma and renal cell carcinoma cells.", BLOOD, vol. 104, 2004, pages 170 - 177
MINGARI, M.C. ET AL.: "In Vitro Proliferation and Cloning of CD3-CD16+ Cells from Human Thymocyte Precursors.", J. EXP. MED., vol. 174, 1991, pages 21 - 26
CARLENS, S. ET AL.: "A New Method for In Vitro Expansion of Cytotoxic Human CD3- CD56+ Natural Killer Cells.", HUMAN IMMUNOLOGY, vol. 62, 2006, pages 1092 - 1098
IGARASHI, T. ET AL.: "Enhanced cytotoxicity of allogeneic NK cells with killer immunoglobulin-like receptor ligand incompatibility against melanoma and renal cell carcinoma cells.", BLOOD, vol. 104, 2004, pages 170 - 177
MINGARI, M.C. ET AL.: "In Vitro Proliferation and Cloning of CD3-CD16+ Cells from Human Thymocyte Precursors.", J. EXP. MED., vol. 174, 1991, pages 21 - 26
Attorney, Agent or Firm:
LEE, Cheo young (Yeosam Bldg. 648-23,Yeoksam-dong, Gangnam-gu, Seoul 135-080, KR)
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