GUIDED MAGNETIC NANOSTRUCTURES FOR TARGETED AND HIGH-THROUGHPUT INTRACELLULAR DELIVERY

Title:

GUIDED MAGNETIC NANOSTRUCTURES FOR TARGETED AND HIGH-THROUGHPUT INTRACELLULAR DELIVERY

Document Type and Number:

WIPO Patent Application WO/2019/148184

Kind Code:

A3

Abstract:

A method of transporting biomolecular cargo intracellularly into cells includes the operations of providing magnetic nanostructures (e.g., nanospears, nanostars, nanorods, and other nanometer-sized structures) carrying the biomolecular cargo thereon and applying an external magnetic field to move the magnetic nanostructures into physical contact with at least some of the cells (or the cells into the magnetic nanostructures). The magnetic nanostructures move into physical contact with a single cell, a subset of cells, or all cells. The external magnetic field may be applied by a moving permanent magnet although an electromagnetic may also be used. The biomolecular cargo may include a molecule, a plurality of molecules, or higher order biological constructs. For example, the biological construct may include DNA plasmids, small interfering RNA, proteins, or targeted nuclease gene-editing cargo such as zinc-finger nucleases, transcription activator-like effector nucleases, Cas9 protein, Cas9 mRNA, and associated guide RNA sequences.

More Like This:

JPS60102963	CLADDING SEPARATOR
WO/2003/039753	SYSTEM AND METHOD FOR CAPTURING AND POSITIONING PARTICLES
JPS58186451	SEPARATOR OF MAGNETIC POWDER AND GRANULE

Inventors:

WEISS PAUL S (US)
TSENG HSIAN-RONG (US)
XU XIAOBIN (US)
WATTANATORN NATCHA (US)
YANG QING (US)
JONAS STEVEN J (US)

Application Number:

PCT/US2019/015650

Publication Date:

April 23, 2020

Filing Date:

January 29, 2019

Export Citation:

Click for automatic bibliography generation Help

Assignee:

UNIV CALIFORNIA (US)

International Classes:

B03C1/24; B01L3/00; G01N33/543

Domestic Patent References:

WO2010076337A1	2010-07-08
WO2017011320A1	2017-01-19
WO2015089427A1	2015-06-18

Foreign References:

US20140045179A1	2014-02-13
US20130011441A1	2013-01-10
US20060105170A1	2006-05-18

Other References: