Title:
HASA-REDOXIN COMPLEX HAVING OXIDATION REACTION, AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2017/038107
Kind Code:
A1
Abstract:
Provided are: a HasA-redoxin complex (a heme iron active-type HasA) which can asymmetrically oxidize only one of enantiomers of a secondary alcohol without the need of adding a coenzyme and has an asymmetric oxidization activity in an aqueous solvent in the presence of oxygen, and an immobilized form of the HasA-redoxin complex; methods respectively for producing the HasA-redoxin complex and the immobilized form of the HasA-redoxin complex; and a production method relating to the coating of the protein complex with a polymeric compound.
Methods respectively for producing a HasA-redoxin complex (a heme iron active-type HasA) having an asymmetric oxidation reaction and an immobilized form thereof. The methods are characterized by comprising: a first step comprising a treatment for encapsulating a plant-originated water-soluble crude protein in a gel, a treatment for oxidizing the gel with air, and a treatment for eluting a HasA-redoxin complex from the gel into an aqueous solution; and a second step comprising a treatment for applying a gravity to the aqueous HasA-redoxin complex solution to concentrate and precipitate the HasA-redoxin complex, a treatment for re-dissolving the HasA-redoxin complex precipitates in an aqueous solution to allow the HasA-redoxin complex and a polymeric compound to co-exist homogeneously, and dehydration/drying of the co-existing aqueous solution to produce a polymeric compound-coated HasA-redoxin complex. The methods are also characterized in that HasA is allowed to express using a transformant (a microorganism) containing HasA originated from Pseudomonas fluorescens, and then the HasA is agitated and oxidized with air in an oxygen-containing aqueous solution, thereby producing a HasA-redoxin complex (a heme iron active-type HasA).
Inventors:
NAGAOKA HIROYUKI (JP)
Application Number:
PCT/JP2016/052213
Publication Date:
March 09, 2017
Filing Date:
January 26, 2016
Export Citation:
Assignee:
SANYO FOODS CO LTD (JP)
International Classes:
C12P7/22; C12N9/02; C12P41/00
Domestic Patent References:
| WO2014073673A1 | 2014-05-15 |
Other References:
NAGAOKA, H.: "An HASApf-redoxin complex causing asymmetric catalytic oxidation via the regenerative formation of a reactive oxygen species", DALTON TRANSACTIONS, vol. 44, no. 29, 7 August 2015 (2015-08-07), pages 13384 - 13393, XP055368814, ISSN: 1477-9226
NAGAOKA, H.: "Application of a Heme-Binding Protein Eluted from Encapsulated Biomaterials to the Catalysis of Enantioselective Oxidation", ACS CATALYSIS, vol. 4, 2014, pages 553 - 565, XP055368823, ISSN: 2155-5435
NAGAOKA, H.: "Application of a Heme-Binding Protein Eluted from Encapsulated Biomaterials to the Catalysis of Enantioselective Oxidation", ACS CATALYSIS, vol. 4, 2014, pages 553 - 565, XP055368823, ISSN: 2155-5435
Attorney, Agent or Firm:
ISHIDA, Takashi et al. (JP)
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