Title:
HEAT-RESISTANT REVERSE TRANSCRIPTASE MUTANT
Document Type and Number:
WIPO Patent Application WO/2018/110595
Kind Code:
A1
Abstract:
Provided are: a reverse transcriptase mutant including an amino acid mutation at a position corresponding to position 55 of the amino acid sequence of wild-type reverse transcriptase derived from the Moloney murine leukemia virus, wherein the reverse transcriptase mutant is characterized in that the amino acid mutation is a substitution from threonine to another amino acid, and the other amino acid is selected from the group consisting of amino acids having a nonpolar aliphatic side chain and amino acids having a polar acidic functional group side chain; a nucleic acid that encodes the mutant; a method for producing the mutant and the nucleic acid that encodes the mutant; a method for synthesizing cDNA in which the mutant is used; and a composition and kit including the mutant.
Inventors:
ISHIKAWA KAZUHIKO (JP)
UEMORI TAKASHI (JP)
TAKATSU NARIAKI (JP)
UEMORI TAKASHI (JP)
TAKATSU NARIAKI (JP)
Application Number:
PCT/JP2017/044693
Publication Date:
June 21, 2018
Filing Date:
December 13, 2017
Export Citation:
Assignee:
TAKARA BIO INC (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/12
Domestic Patent References:
| WO2015112767A2 | 2015-07-30 | |||
| WO2012020759A1 | 2012-02-16 | |||
| WO2004024749A2 | 2004-03-25 | |||
| WO2007022045A2 | 2007-02-22 | |||
| WO2009125006A2 | 2009-10-15 | |||
| WO2012108672A2 | 2012-08-16 | |||
| WO2015112767A2 | 2015-07-30 |
Foreign References:
| JP2009504162A | 2009-02-05 | |||
| JP2004511211A | 2004-04-15 | |||
| JP2011516072A | 2011-05-26 | |||
| JPH04193079A | 1992-07-13 |
Other References:
HISAYOSHI, TETSURO ET AL.: "Heat resistance of MMLV reverse transcriptase by amino acid mutations to region spaced apart from Rnase H catalyst region, and disappearance of RNase H activation", LECTURE ABSTRACTS IN MEETING OF JAPAN SOCIETY OF BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, vol. 2015, 2015, pages 4C22p03, XP009515184, ISSN: 2186-7976
Attorney, Agent or Firm:
SAMEJIMA, Mutsumi et al. (JP)
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