Title:
HEAT-RESISTANT XYLANASE
Document Type and Number:
WIPO Patent Application WO/2016/175202
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a heat-resistant xylanase that is capable of retaining activity with long-term exposure to high-temperature conditions during conventional biomass saccharification steps. Provided is a xylanase comprising a polypeptide formed from an amino acid sequence represented by Sequence No. 2, where the amino acid sequence has mutation at at least one amino acid selected from the group consisting of the 21st threonine, 97th asparagine, 242nd glutamine, and 296th asparagine of the amino acid sequence.
Inventors:
YAOI KATSURO (JP)
MATSUZAWA TOMOHIKO (JP)
MATSUZAWA TOMOHIKO (JP)
Application Number:
PCT/JP2016/063034
Publication Date:
November 03, 2016
Filing Date:
April 26, 2016
Export Citation:
Assignee:
NAT INST ADVANCED IND SCIENCE & TECH (JP)
International Classes:
C12N9/24; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12P5/00; C12P19/14
Domestic Patent References:
| WO2014019219A1 | 2014-02-06 |
Other References:
DATABASE UniProtKB [o] 22 February 2012 (2012-02-22), KUBICEK C. P. ET AL.: "Definition: Beta-xylanase.", Database accession no. G9MUR3
TSUKIMURA W ET AL.: "Improvement of alkaliphily of thermostable GH family 10 xylanase from Thermotoga maritima by directed evolution.", JOURNAL OF JAPANESE SOCIETY FOR EXTREMOPHILES, vol. 9, no. 1, 2010, pages 15 - 18, XP055326890
RULLER R. ET AL.: "Concommitant adaptation of a GH11 xylanase by directed evolution to create an alkali-tolerant/thermophilic enzyme.", PROTEIN ENG DES SEL, vol. 27, no. 8, 2014, pages 255 - 262, XP055326891
MIYAZAKI K. ET AL.: "Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution.", J BIOL CHEM, vol. 281, no. 15, 2006, pages 10236 - 10242, XP055326892
ARASE A. ET AL.: "Stabilization of xylanase by random mutagenesis.", FEBS LETT, vol. 316, no. 2, 1993, pages 123 - 127, XP025592168
KATSURO YAOI ET AL.: "Shinka Bunshi Kogaku ni yoru Trichoderma reesei Yurai Xylanase III (XynIII) no Tainetsuka", BULLETIN OF APPLIED GLYCOSCIENCE, vol. 5, no. 3, August 2015 (2015-08-01), pages 44 Bp - 8
TOMOHIKO MATSUZAWA ET AL.: "Enhancement of thermostability of Trichoderma reesei XynIII", ABSTRACTS OF THE ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, vol. 67, September 2015 (2015-09-01), Japan, pages 181 2P-027
CHEN CC. ET AL.: "Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability.", J BIOTECHNOL, vol. 189, 2014, pages 175 - 182, XP029089452
WANG J. ET AL.: "Improving the thermostability of a mesophilic family 10 xylanase, AuXyn10A, from Aspergillus usamii by in silico design.", J IND MICROBIOL BIOTECHNOL, vol. 41, no. 8, 2014, pages 1217 - 1225, XP055326897
MATSUZAWA T. ET AL.: "Improvement of thermostability and activity of Trichoderma reesei endo-xylanase Xyn III on insoluble substrates.", APPL MICROBIOL BIOTECHNOL, 30 April 2016 (2016-04-30), XP036034117, Retrieved from the Internet [retrieved on 20160711]
TSUKIMURA W ET AL.: "Improvement of alkaliphily of thermostable GH family 10 xylanase from Thermotoga maritima by directed evolution.", JOURNAL OF JAPANESE SOCIETY FOR EXTREMOPHILES, vol. 9, no. 1, 2010, pages 15 - 18, XP055326890
RULLER R. ET AL.: "Concommitant adaptation of a GH11 xylanase by directed evolution to create an alkali-tolerant/thermophilic enzyme.", PROTEIN ENG DES SEL, vol. 27, no. 8, 2014, pages 255 - 262, XP055326891
MIYAZAKI K. ET AL.: "Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution.", J BIOL CHEM, vol. 281, no. 15, 2006, pages 10236 - 10242, XP055326892
ARASE A. ET AL.: "Stabilization of xylanase by random mutagenesis.", FEBS LETT, vol. 316, no. 2, 1993, pages 123 - 127, XP025592168
KATSURO YAOI ET AL.: "Shinka Bunshi Kogaku ni yoru Trichoderma reesei Yurai Xylanase III (XynIII) no Tainetsuka", BULLETIN OF APPLIED GLYCOSCIENCE, vol. 5, no. 3, August 2015 (2015-08-01), pages 44 Bp - 8
TOMOHIKO MATSUZAWA ET AL.: "Enhancement of thermostability of Trichoderma reesei XynIII", ABSTRACTS OF THE ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, vol. 67, September 2015 (2015-09-01), Japan, pages 181 2P-027
CHEN CC. ET AL.: "Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability.", J BIOTECHNOL, vol. 189, 2014, pages 175 - 182, XP029089452
WANG J. ET AL.: "Improving the thermostability of a mesophilic family 10 xylanase, AuXyn10A, from Aspergillus usamii by in silico design.", J IND MICROBIOL BIOTECHNOL, vol. 41, no. 8, 2014, pages 1217 - 1225, XP055326897
MATSUZAWA T. ET AL.: "Improvement of thermostability and activity of Trichoderma reesei endo-xylanase Xyn III on insoluble substrates.", APPL MICROBIOL BIOTECHNOL, 30 April 2016 (2016-04-30), XP036034117, Retrieved from the Internet
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