Title:
HEAT-STERILIZABLE NATTO
Document Type and Number:
WIPO Patent Application WO/2017/104637
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for acquiring Bacillus subtilis var. natto which has a markedly low sporulation capacity upon fermenting soybeans and using this bacterium to produce natto with a low content of Bacillus subtilis var. natto spores using existing equipment and processes without the problems of fermentation failures and bacterial contamination. The present invention provides a mutant strain of Bacillus subtilis var. natto that, relative to a sporulation capacity of 100% in a culture medium comprising at least 200 μg/ml of dipicolinic acid, has a sporulation capacity of no higher than 5% in a culture medium that does not include dipicolinic acid, and has the quality of being able to produce natto which has excellent filament formation and appearance. The present invention also provides natto produced using said mutant strain, and a natto production method using said mutant strain.
Inventors:
KADA SHIGEKI (JP)
Application Number:
PCT/JP2016/086996
Publication Date:
June 22, 2017
Filing Date:
December 13, 2016
Export Citation:
Assignee:
MIZKAN HOLDINGS CO LTD (JP)
International Classes:
C12N1/21; A23L11/00; C12N15/09
Domestic Patent References:
| WO2007125783A1 | 2007-11-08 | |||
| WO2006025477A1 | 2006-03-09 |
Foreign References:
| JP2009017842A | 2009-01-29 |
Other References:
MITSUI, NOBUO ET AL.: "Development of natto with germination-defective mutants of Bacillus subtilis (natto", APPL MICROBIOL BIOTECHNOL, vol. 82, no. 4, March 2009 (2009-03-01), pages 741 - 748, XP019705538
PAIDHUNGAT, MADAN ET AL.: "Characterization of Spores of Bacillus subtilis Which Lack Dipicolinic Acid", JOURNAL OF BACTERIOLOGY, vol. 182, no. 19, October 2000 (2000-10-01), pages 5505 - 5512, XP055397460
DANIEL, R, A ET AL.: "Cloning, DNA Sequence, Functional Analysis and Transcriptional Regulation of the Genes Encoding Dipicolinic Acid Synthetase Required for Sporulation in Bacillus", J MOL BIOL, vol. 232, no. 2, 1993, pages 468 - 483, XP024008919
LO, CHIEN-CHI ET AL.: "Cohesion Group Approach for Evolutionary Analysis of Aspartokinase, an Enzyme That Feeds a Branched Network of Many Biochemical Pathways", MIRCOBIOL MOL BIOL REV, vol. 73, no. 4, 2009, pages 594 - 600, XP055397465
PAIDHUNGAT, MADAN ET AL.: "Characterization of Spores of Bacillus subtilis Which Lack Dipicolinic Acid", JOURNAL OF BACTERIOLOGY, vol. 182, no. 19, October 2000 (2000-10-01), pages 5505 - 5512, XP055397460
DANIEL, R, A ET AL.: "Cloning, DNA Sequence, Functional Analysis and Transcriptional Regulation of the Genes Encoding Dipicolinic Acid Synthetase Required for Sporulation in Bacillus", J MOL BIOL, vol. 232, no. 2, 1993, pages 468 - 483, XP024008919
LO, CHIEN-CHI ET AL.: "Cohesion Group Approach for Evolutionary Analysis of Aspartokinase, an Enzyme That Feeds a Branched Network of Many Biochemical Pathways", MIRCOBIOL MOL BIOL REV, vol. 73, no. 4, 2009, pages 594 - 600, XP055397465
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Download PDF: