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Title:
HEDGEHOG INHIBITOR ASSAY
Document Type and Number:
WIPO Patent Application WO2006080894
Kind Code:
A3
Abstract:
The present invention relates to the field of Hedgehog signalling, and more specifically to a cell-based assay system and methods for identifying inhibitors and/or antagonists of specific cellular events in said signalling pathway. The present invention hereby proposes a novel approach for identifying inhibitors and/or antagonists downstream of the signalling components Smoothened and Patched, e.g. at the Gli-level. The assay comprises cells lacking a functional Sufu protein, which according to the invention is a protein component of emerging importance in the Hedgehog signalling pathway.

Inventors:
TOFTGAARD RUNE (SE)
LAUTH MATTIAS (SE)
TEGLUND STEPHAN (SE)
Application Number:
SE2006000141W
Publication Date:
November 09, 2006
Filing Date:
January 31, 2006
Export Citation:
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Assignee:
KAROLINSKA INST INNOVATIONS AB (SE)
TOFTGAARD RUNE (SE)
LAUTH MATTIAS (SE)
TEGLUND STEPHAN (SE)
International Classes:
G01N33/50; C12Q1/66
Domestic Patent References:
WO2002036818A22002-05-10
Foreign References:
CA2432496A12003-12-14
Other References:
COOPER AF: "Targeted disruption of mouse suppressor of fused (Sufu), a negative regulator of hedgehog (Hh) signaling", CNIO 2004 SYMPOSIUM, 6 February 2004 (2004-02-06), XP002384359, Retrieved from the Internet [retrieved on 20060607]
ANONYMOUS: "C3H/10T1/2", HYPERCLDB, 2002, XP002384360, Retrieved from the Internet [retrieved on 20060608]
REZNIKOFF C A ET AL: "ESTABLISMENT AND CHARACTERIZATION OF A CLONED LINE OF C3H MOUSE EMBRYO CELLS SENTITIVE TO POSTCONFLUENCE INHIBITION OF DIVISION", CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, BALTIMORE, MD, US, vol. 33, no. 12, December 1973 (1973-12-01), pages 3231 - 3238, XP008053213, ISSN: 0008-5472
SVÄRD JESSICA ET AL: "Genetic elimination of Suppressor of fused reveals an essential repressor function in the mammalian Hedgehog signaling pathway.", DEVELOPMENTAL CELL. FEB 2006, vol. 10, no. 2, February 2006 (2006-02-01), pages 187 - 197, XP008065095, ISSN: 1534-5807
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