Title:
HEPAROSAN-PRODUCING BACTERIUM AND HEPAROSAN MANUFACTURING METHOD
Document Type and Number:
WIPO Patent Application WO/2015/050184
Kind Code:
A1
Abstract:
Provided is a heparosan manufacturing method. Heparosan is manufactured by culturing in a medium an Escherichia sp. bacterium with heparosan-producing ability, the bacterium having been modified so that expression of one or more genes selected from a group consisting of rbsR, rbsK, rbsB, hsrA, glgB, glgX, micF, rcsD, rcsB, ybiX, ybiI, ybiJ, ybiC, ybiB, rfaH, nusG, pcoR, pcoS, pcoE, yhcN, yhcO, aaeB, aaeA, aaeX, g1455, alpA, g1453, yrbA, mlaB, mlaC, mlaD, mlaE, mlaF, yrbG, norW, ybjI, ybjJ, ybjK, rybB, yjjY, yjtD, thrL, thrA, thrB, fruA, psuK, ytfT, yjfF, fbp, yagU, paoA, paoB, gsiC, gsiD, yliE, irp2, irp1, bhsA, ycfS, lepB, rnc, era, dapA, gcvR, bcp, hyfA, rpoE, nadB, yfiC, srmB, g1414, g1413, nuoE, nuoF, nuoG, glmZ, hemY, hemX, hemD, rlmL, artQ, artM, artJ, rlmC, ybjO, yejO, yejM, yejL, rpoS, ygbN, ygbM, ygbL, g3798, g3797, g3796, g3795, g3794, g3793, g3792, ryjA, soxR, soxS, yjcC, yjcB, efeU, and efeO genes is increased, and collecting the heparosan from said medium.
More Like This:
Inventors:
YAMAZAKI SHUNSUKE (JP)
SHIMIZU TOMOKO (JP)
MORI KENICHI (JP)
TONOUCHI NAOTO (JP)
SHIMIZU TOMOKO (JP)
MORI KENICHI (JP)
TONOUCHI NAOTO (JP)
Application Number:
PCT/JP2014/076357
Publication Date:
April 09, 2015
Filing Date:
October 02, 2014
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12N1/21; C12N15/09; C12P19/04
Domestic Patent References:
| WO2010136435A1 | 2010-12-02 | |||
| WO2009014559A2 | 2009-01-29 | |||
| WO2000018935A1 | 2000-04-06 | |||
| WO2010027045A1 | 2010-03-11 | |||
| WO2005010175A1 | 2005-02-03 | |||
| WO2010027022A1 | 2010-03-11 |
Foreign References:
| US20110111458A1 | 2011-05-12 | |||
| JP2013503606A | 2013-02-04 | |||
| JPH02109985A | 1990-04-23 | |||
| US5882888A | 1999-03-16 | |||
| EP0805867B1 | 2003-12-17 | |||
| RU2006134574A | 2008-04-10 | |||
| JPH02207791A | 1990-08-17 | |||
| US20050196846A1 | 2005-09-08 | |||
| JP2008099668A | 2008-05-01 |
Other References:
ZHANG C. ET AL.: "Metabolic engineering of Escherichia coli BL21 for biosynthesis of heparosan, a bioengineered heparin precursor.", METABOLIC ENGINEERING, vol. 14, 2012, pages 521 - 527, XP055144554
KANG M.J. ET AL.: "Identification of genes affecting lycopene accumulation in Escherichia coli using a shot-gun method.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 91, 2005, pages 636 - 642, XP055333964
SANTOS C.N.S. ET AL.: "Rational, combinatorial, and genomic approaches for engineering tyrosine production in Escherichia coli.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 109, no. 34, 2012, pages 13538 - 13543, XP055333965
BAILEY M.J.A. ET AL.: "RfaH and the ops element, components of a novel system controlling bacterial transcription elongation.", MOLECULAR MICROBIOLOGY, vol. 26, no. 5, 1997, pages 845 - 851, XP055333969
LINDAHL U ET AL., J. BIOL. CHEM., vol. 273, no. 39, 1998, pages 24979 - 24982
HODSON N. ET AL., J. BIOL. CHEM., vol. 275, no. 35, 2000, pages 27311 - 27315
MCNULTY C. ET AL., MOL. MICROBIOL., vol. 59, no. 3, 2006, pages 907 - 22
JANN B.; JANN K., CURR. TOP MICROBIOL. IMMUNOL., vol. 150, 1990, pages 19 - 42
KANE T.A. ET AL., J. BIOL. CHEM., vol. 281, no. 44, 3 November 2006 (2006-11-03), pages 33192 - 33197
LINDAHL U. ET AL., J. MED. CHEM., vol. 48, no. 2, 2005, pages 349 - 352
ZHANG Z. ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 130, no. 39, 2008, pages 12998 - 13007
WANG Z. ET AL., BIOTECHNOL. BIOENG, vol. 107, no. 6, 2010, pages 964 - 973
ZANG C ET AL., METABOLIC ENGINEERING, vol. 14, no. 5, 2012, pages 521 - 527
LAIKOVA O.N. ET AL.: "Computational analysis of the transcriptional regulation of pentose utilization systems in the gamma subdivision of Proteobacteria", FEMS MICROBIOL LETT., vol. 205, no. 2, 2001, pages 315 - 22, XP027360432
BORK P. ET AL.: "Convergent evolution of similar enzymatic function on different protein folds: the hexokinase, ribokinase, and galactokinase families of sugar kinases", PROTEIN SCI., vol. 2, no. 1, 1993, pages 31 - 40
IIDA A. ET AL.: "Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12", J. BACTERIOL., vol. 158, no. 2, 1984, pages 674 - 82
PAO S.S ET AL.: "Major facilitator superfamily", MICROBIOL. MOL. BIOL. REV., vol. 62, no. 1, 1998, pages 1 - 34, XP002203285
BOYER C.; PREISS: "Biosynthesis of bacterial glycogen: Purification and properties of the Escherichia coli b alpha-1,4,-glucan: alpha-1,4-glucan 6-glycosyltansferase", J. BIOCHEMISTRY, vol. 16, no. 16, 1977, pages 3693 - 9, XP002995361, DOI: doi:10.1021/bi00635a029
DAUVILLEE D ET AL.: "Role of the Escherichia coli glgX gene in glycogen metabolism", J. BACTERIOL., vol. 187, no. 4, 2005, pages 1465 - 73
RAMANI N.: "micF antisense RNA has a major role in osmoregulation of OmpF in Escherichia coli", J. BACTERIOL., vol. 176, 1994, pages 5005 - 5010
MAJDALANI N. ET AL.: "The Rcs phosphorelay: a complex signal transduction system", ANUU. REV. MICROBIOL., vol. 59, 2005, pages 379 - 405
HOUNG H.S ET AL.: "Expression of Vi antigen in Escherichia coli K-12: characterization of ViaB from Citrobacter freundii and identity of ViaA with RcsB", J. BACTERIOL., vol. 174, 1992, pages 5910 - 5915
ROCHAPORN W. ET AL.: "Involvement of rcsB in Klebsiella K2 Capsule Synthesis in Escherichia coli K-12", J. BACTERIOL., vol. 174, 1992, pages 1063 - 1067
ANDREA RAHN ET AL.: "Transcriptional organization and regulation of The Escherichia coli", MOL. MICROBIOL., vol. 47, 2003, pages 1045 - 1060
WENDY J. KEENLEYSIDE ET AL.: "Coexpression of Colanic Acid and Serotype-Specific Capsular Polysaccharides in Escherichia coli Strains with Group II K Antigens", J. BACTERIOL., vol. 175, 1993, pages 6725 - 6730, XP000579304
LEEDS J.A.; D WELCH R.A.: "RfaH enhances elongation of Escherichia coli hlyCABD mRNA", J. BACTERIOL., vol. 178, no. 7, 1996, pages 1850 - 7, XP002472460
TEVENS M.P ET AL.: "Regulation of Escherichia coli K5 capsular polysaccharide expression: Evidence for involvement of RfaH in the expression of group II capsules", FEMS MICROBIOL. LETT., vol. 124, no. 1, 1994, pages 93 - 98
XUE P. ET AL.: "Regulation of expression of the region 3 promoter of the Escherichia coli K5 capsule gene cluster involves H-NS, SlyA, and a large 5' untranslated region", J. BACTERIOL., vol. 191, no. 6, 2009, pages 1838 - 1846
LI J. ET AL., J. BIOL. CHEM., vol. 267, no. 9, 1992, pages 6012 - 6019
LIVANIS M ET AL., J. BACTERIOL., vol. 191, no. 23, 2009, pages 7288 - 7295
BAILEY M. ET AL., MOL. MICROBIOL., vol. 22, no. 4, 1996, pages 7729 - 737
COOKSEY D.A.: "Copper uptake and resistance in bacteria", MOL. MICROBIOL., vol. 7, no. 1, 2006, pages 1 - 5
LEE J. ET AL.: "Identification of stress-related proteins in Escherichia coli using the pollutant cis-dichloroethylene", J. APPL. MICROBIOL., vol. 108, no. 6, June 2010 (2010-06-01), pages 2088 - 102
VAN DYK T.K. ET AL.: "Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?", J. BACTERIOL., vol. 186, 2004, pages 7196 - 7204
TREMPY J.E. ET AL.: "Alp suppression of Lon: dependence on the slpA gene", J. BACTERIOL., vol. 176, no. 7, 1994, pages 2061 - 7, XP000938361
HERZBERG M. ET AL.: "YdgG (TqsA) controls biofilm formation in Escherichia coli K-12 through autoinducer 2 transport", J. BACTERIOL., vol. 188, no. 2, 2006, pages 587 - 98
GUINOTE I.B. ET AL.: "Characterization of the BolA homolog IbaG: a new gene involved in acid resistance", J. MICROBIOL. BIOTECHNOL, vol. 22, no. 4, 2012, pages 484 - 93
MALINVERNI J.C.; SILHAVY T.J: "An ABC transport system that maintains lipid asymmetry in the gram-negative outer membrane", PROC. NATL. ACAD. SCI. USA, vol. 106, no. 19, 2009, pages 8009 - 14
NASEEM R ET AL.: "pH and monovalent cations regulate cytosolic free Ca(2+) in E. coli", BIOCHIM. BIOPHYS. ACTA, vol. 1778, no. 6, 2008, pages 1415 - 22, XP022673952, DOI: doi:10.1016/j.bbamem.2008.02.006
GARDNER A.M. ET AL.: "Role of NorR and sigma54 in the nitric oxide stress response", J. BIOL. CHEM., vol. 278, no. 12, 2003, pages 10081 - 6
KUZNETSOVA E. ET AL.: "Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family", J. BIOL. CHEM., vol. 281, no. 47, 2006, pages 36149 - 61, XP055038714, DOI: doi:10.1074/jbc.M605449200
THOMPSON K.M. ET AL.: "SigmaE regulates and is regulated by a small RNA in Escherichia coli", J. BACTERIOL., vol. 189, no. 11, 2007, pages 4243 - 56
JOHANSEN J. ET AL.: "Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteins", J. MOL. BIOL., vol. 364, no. 1, 2006, pages 1 - 8
ANANTHARAMAN V ET AL.: "SPOUT: a class of methyltransferases that includes spoU and trmD RNA methylase superfamilies, and novel superfamilies of predicted prokaryotic RNA methylases", J. MOL. MICROBIOL. BIOTECHNOL., vol. 4, no. 1, 2002, pages 71 - 5
BURR B. ET AL.: "Homoserine kinase from Escherichia coli K12", EUR. J. BIOCHEM., vol. 62, no. 3, 1976, pages 519 - 26
CLARK R.B.; OGILVIE J.W. ET AL.: "Aspartokinase I-homoserine dehydrogenase I of Escherichia coli K12: Subunit molecular weight and nicotinamide-adenine dinucleotide phosphate binding", BIOCHEMISTRY, vol. 11, no. 7, 1972, pages 1278 - 82
LYNN S.P. ET AL.: "Attenuation regulation in the thr operon of Escherichia coli K-12: molecular cloning and transcription of the controlling region", J. BACTERIOL., vol. 152, no. L, 1982, pages 363 - 71
PRIOR T.I.; KORNBERG H.L.: "Nucleotide sequence of fruA, the gene specifying enzyme IIfru of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Escherichia coli K12", J. GEN. MICROBIOL., vol. 134, no. 10, 1988, pages 2757 - 68, XP002937888
SOLOMON L.R; BREITMAN T.R: "Pseudouridine kinase of Escherichia coli: a new enzyme", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 44, no. 2, 1971, pages 299 - 304, XP024778957, DOI: doi:10.1016/0006-291X(71)90599-7
FRAENKEL D.G; HORECKER B.L.: "Fructose-1,6-diphosphatase and acid hexose phosphatase of Escherichia coli", J. BACTERIOL., vol. 90, no. 4, 1965, pages 837 - 42
NEUMANN M. ET AL.: "A periplasmic aldehyde oxidoreductase represents the first molybdopterin cytosine dinucleotide cofactor containing molybdo-flavoenzyme from Escherichia coli", FEBS J., vol. 276, no. 10, 2009, pages 2762 - 74
MOUSSATOVA A. ET AL.: "ATP-binding cassette transporters in Escherichia coli", BIOCHIM. BIOPHYS. ACTA, vol. 1778, no. 9, 2008, pages 1757 - 71, XP025408804, DOI: doi:10.1016/j.bbamem.2008.06.009
BOEHM A ET AL.: "Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress", MOL. MICROBIOL., vol. 72, no. 6, 2009, pages 1500 - 16
PELLUDAT C ET AL.: "The yersiniabactin biosynthetic gene cluster of Yersinia enterocolitica: organization and siderophore-dependent regulation", J. BACTERIOL., vol. 180, no. 3, 1998, pages 538 - 46
ZHANG X.S. ET AL.: "YcfR (BhsA) influences Escherichia coli biofilm formation through stress response and surface hydrophobicity", J. BACTERIOL., vol. 189, no. 8, 2007, pages 3051 - 62
MAGNET S. ET AL.: "Identification of the L,D-transpeptidases responsible for attachment of the Braun lipoprotein to Escherichia coli peptidoglycan", J. BACTERIOL., vol. 189, no. 10, 2007, pages 3927 - 31
DALBEY R.E.: "Leader peptidase", MOL. MICROBIOL., vol. 5, no. 12, 1991, pages 2855 - 60
ROBERTSON H.D; DUNN J.J.: "Ribonucleic acid processing activity of Escherichia coli ribonuclease III", J. BIOL. CHEM., vol. 250, no. 8, 25 December 1974 (1974-12-25), pages 3050 - 6
TAKIFF H.E ET AL.: "Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon", J. BACTERIOL., vol. 174, no. 5, 1992, pages 1544 - 53, XP000673194
ZHANG J.; INOUYE M.: "MazG, a nucleoside triphosphate pyrophosphohydrolase, interacts with Era, an essential GTPase in Escherichia coli", J. BACTERIOL., vol. 184, no. 19, 2002, pages 5323 - 9
LABER B ET AL.: "Escherichia coli dihydrodipicolinate synthase: Identification of the active site and crystallization", BIOCHEM. J., vol. 288, 1992, pages 691 - 5, XP009035909
GHRIST A.C. ET AL.: "GcvR interacts with GcvA to inhibit activation of the Escherichia coli glycine cleavage operon", MICROBIOLOGY, vol. 147, 2001, pages 2215 - 21
CLARKE D.J. ET AL.: "Interrogating the molecular details of the peroxiredoxin activity of the Escherichia coli bacterioferritin comigratory protein using high-resolution mass spectrometry", BIOCHEMISTRY, vol. 48, no. 18, 2009, pages 3904 - 14
ANDREWS S.C. ET AL.: "A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system", MICROBIOLOGY, vol. 143, 1997, pages 3633 - 47
ADES S.E. ET AL.: "Regulation of the alternative sigma factor sigma(E) during initiation, adaptation, and shutoff of the extracytoplasmic heat shock response in Escherichia coli", J. BACTERIOL., vol. 185, no. 8, 2003, pages 2512 - 9
MORTARINO M ET AL.: "L-aspartate oxidase from Escherichia coli, I. Characterization of coenzyme binding and product inhibition", EUR. J. BIOCHEM., vol. 239, no. 2, 1996, pages 418 - 26, XP055153570, DOI: doi:10.1111/j.1432-1033.1996.0418u.x
GOLOVINA A.Y. ET AL., THE YFIC GENE OF E. COLI ENCODES AN ADENINE-N6 METHYLTRANSFERASE THAT SPECIFICALLY MODIFIES A37 OF TRNALVAL(CMO5UAC, vol. 15, no. 6, 2009, pages 1134 - 41
CHAROLLAIS J. ET AL.: "The DEAD-box RNA helicase SrmB is involved in the assembly of 50S ribosomal subunits in Escherichia coli", MOL. MICROBIOL., vol. 48, no. 5, 2003, pages 1253 - 65
BRAUN M ET AL.: "Characterization of the overproduced NADH dehydrogenase fragment of the NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli", BIOCHEMISTRY, vol. 37, no. 7, 1998, pages 1861 - 7
KALAMORZ F. ET AL.: "Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli", MOL. MICROBIOL., vol. 65, no. 6, 2007, pages 1518 - 33
URBAN J.H.; VOGEL J. ET AL.: "Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation", PLOS BIOL., vol. 6, no. 3, 2008, pages E64
DAILEY T.A. ET AL.: "Expression of a cloned protoporphyrinogen oxidase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, 1994, pages 813 - 815
SASARMAN A ET AL.: "Nucleotide sequence of the hemX gene, the third member of the Uro operon of Escherichia coli K12", NUCLEIC ACIDS RES., vol. 16, no. 24, 1988, pages 11835
JORDAN P.M.; WOODCOCK S.C.: "Mutagenesis of arginine residues in the catalytic cleft of Escherichia coli porphobilinogen deaminase that affects dipyrromethane cofactor assembly and tetrapyrrole chain initiation and elongation", BIOCHEM. J., vol. 280, 1991, pages 445 - 9
KIMURA S ET AL.: "Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity", NUCLEIC ACIDS RES., vol. 40, no. 9, 2012, pages 4071 - 85
LINTON K.J.; HIGGINS C.F.: "The Escherichia coli ATP-binding cassette (ABC) proteins", MOL. MICROBIOL., vol. 28, no. 1, 1998, pages 5 - 13
MADSEN C.T. ET AL.: "Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry", NUCLEIC ACIDS RES., vol. 31, no. 16, 2003, pages 4738 - 46
RAPP M. ET AL.: "Experimentally based topology models for E. coli inner membrane proteins", PROTEIN SCI., vol. 13, no. 4, 2004, pages 937 - 45
HENDERSON I.R; OWEN P.: "The major phase-variable outer membrane protein of Escherichia coli structurally resembles the immunoglobulin A1 protease class of exported protein and is regulated by a novel mechanism involving Dam and oxyR", J. BACTERIOL., vol. 181, no. 7, 1999, pages 2132 - 41
MACIAG A ET AL.: "In vitro transcription profiling of the cyS subunit of bacterial RNA polymerase: Re-definition of the ?S regulon and identification of oS-specific promoter sequence elements", NUCLEIC ACIDS RES., vol. 39, no. 13, 2011, pages 5338 - 55
PEEKHAUS N. ET AL.: "Characterization of a novel transporter family that includes multiple Escherichia coli gluconate transporters and their homologues", FEMS MICROBIOL. LETT., vol. 147, no. 2, 1997, pages 233 - 8, XP002204792, DOI: doi:10.1016/S0378-1097(96)00532-0
WASSARMAN K.M. ET AL.: "Identification of novel small RNAs using comparative genomics and microarrays", GENES, DEV., vol. 15, no. 13, 2001, pages 1637 - 51, XP002223289, DOI: doi:10.1101/gad.901001
GU M.; IMLAY J.A.: "The SoxRS response of Escherichia coli is directly activated by redox-cycling drugs rather than by superoxide", MOL. MICROBIOL., vol. 79, no. 5, 2011, pages 1136 - 50
TOUATI D.: "Sensing and protecting against superoxide stress in Escherichia coli -- how many ways are there to trigger soxRS response?", REDOX REP., vol. 5, no. 5, 2000, pages 287 - 93
LEE J.H ET AL.: "SoxRS-mediated lipopolysaccharide modification enhances resistance against multiple drugs in Escherichia coli", J. BACTERIOL., vol. 191, no. 13, 2009, pages 4441 - 50
BOEHM A ET AL.: "Second messenger signaling governs Escherichia coli biofilm induction upon ribosomal stress", MOL. MICROBIOL., vol. 72, no. 6, 2009, pages 1500 - 16
CAO J. ET AL.: "EfeUOB (YcdNOB) is a tripartite, acid-induced and CpxAR-regulated, low-pH Fe2+ transporter that iscryptic in Escherichia coli K-12 but functional in E. coli 0-157:H7", MOL. MICROBIOL., vol. 65, 2007, pages 857 - 875
BACKMANN B.J.: "Escherichia coli and Salmonella Cellular and Molecular Biology", 1996, AMERICAN SOCIETY FOR MICROBIOLOGY PRESS, article "Derivations and Genotypes of some mutant derivatives of Escherichia coli K-12", pages: 2460 - 2488
CADWELL, R.C., PCR METH. APPL., vol. 2, 1992, pages 28
STEMMER, W.P., NATURE, vol. 370, 1994, pages 389
ZHAO, H., NATURE BIOTECHNOL., vol. 16, 1998, pages 258
MILLER, J.H.: "Experiments in Molecular Genetics", 1972, COLD SPRING HARBOR LABORATORY
DATSENKO, K.A.; WANNER, B.L: "Proc. Natl. Acad. Sci. USA", vol. 97, 2000, pages: 6640 - 6645
"Genetic Engineering", vol. 8, 1987, KYORITSU SHUPPAN CO., LTD, article "Fundamental Microbiology"
GENE, vol. 60, no. 1, 1987, pages 115 - 127
GOLDSTEIN ET AL.: "Prokaryotic Promoters in Biotechnology", BIOTECHNOL. ANNU. REV., vol. 1, 1995, pages 105 - 128, XP008038474
OLINS P.O. ET AL., GENE, vol. 73, 1988, pages 227 - 235
KANE, J.F., CURR. OPIN. BIOTECHNOL., vol. 6, no. 5, 1995, pages 494 - 500
HIGUCHI, R.: "PCR Technology", vol. 61, 1989, STOCKTON PRESS
CARTER, P., METH. IN ENZYMOL., vol. 154, 1987, pages 382
KRAMER, W.; FRITS, H.J., METH. IN ENZYMOL, vol. 154, 1987, pages 350
KUNKEL, T.A. ET AL., METH. IN ENZYMOL., vol. 154, 1987, pages 367
NAKAMURA, Y ET AL., NUCL. ACIDS RES., vol. 28, 2000, pages 292
MANDEL, M.; HIGA, A., J. MOL. BIOL., vol. 53, 1970, pages 159 - 162
DUNCAN, C.H.; WILSON, G.A; YOUNG, F.E., GENE, vol. 1, 1977, pages 153 - 167
CHANG, S; CHOEN, S.N., MOL. GEN. GENET., vol. 168, 1979, pages 111 - 115
BIBB, M.J.; WARD, J.M; HOPWOOD, O.A., NATURE, vol. 274, 1978, pages 398 - 400
HINNEN, A.; HICKS, J.B.; FINK, G.R., PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929 - 1933
SAMBROOK, J. ET AL.: "Molecular Cloning A Laboratory Manual, 3rd ed.", 2001, COLD SPRING HARBOR LABORATORY PRESS
"Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
BITTER T.; MUIR H.M.: "A modified uronic acid carbazole reaction", ANALYTICAL BIOCHEMISTRY, vol. 4, no. 4, 1962, pages 330 - 334, XP024827447, DOI: doi:10.1016/0003-2697(62)90095-7
ZHANG Z. ET AL.: "Solution Structures of Chemoenzymatically Synthesized Heparin and Its Precursors", J. AM. CHEM. SOC., vol. 130, no. 39, 2008, pages 12998 - 13007, XP055237054, DOI: doi:10.1021/ja8026345
KUBERAN B. ET AL.: "Chemoenzymatic Synthesis of Classical and Non-classical Anticoagulant Heparan Sulfate Polysaccharides", J. BIOL. CHEM., vol. 278, 2003, pages 52613 - 52621
KUBERAN B. ET AL.: "Chemoenzymatic Synthesis of Classical and Non-classical Anticoagulant Heparan Sulfate Polysaccharides", J. BIOL. CHEM., vol. 278, no. 52, 2003, pages 52613 - 52621
CHEN J. ET AL.: "Enzymatic redesigning of biologically active heparan sulfate", J. BIOL. CHEM., vol. 280, no. 52, 30 December 2005 (2005-12-30), pages 42817 - 25, XP002609045, DOI: doi:10.1074/JBC.M504338200
BITTER, T.; MURIR H.M., ANAL. BIOCHEM., vol. 4, 1962, pages 330 - 334
AMANN E. ET AL.: "Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli", GENE, vol. 25, no. 2-3, 1983, pages 167 - 78, XP023599405, DOI: doi:10.1016/0378-1119(83)90222-6
DATSENKO; WANNER: "One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products", PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 6645, XP002210218, DOI: doi:10.1073/pnas.120163297
DATSENKO; WANNER, PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 45
SAMBROOK, J. ET AL.: "Molecular Cloning A Laboratory Manual, 2nd ed.", 1989, COLD SPRING HARBOR LABORATORY PRESS
KANG M.J. ET AL.: "Identification of genes affecting lycopene accumulation in Escherichia coli using a shot-gun method.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 91, 2005, pages 636 - 642, XP055333964
SANTOS C.N.S. ET AL.: "Rational, combinatorial, and genomic approaches for engineering tyrosine production in Escherichia coli.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 109, no. 34, 2012, pages 13538 - 13543, XP055333965
BAILEY M.J.A. ET AL.: "RfaH and the ops element, components of a novel system controlling bacterial transcription elongation.", MOLECULAR MICROBIOLOGY, vol. 26, no. 5, 1997, pages 845 - 851, XP055333969
LINDAHL U ET AL., J. BIOL. CHEM., vol. 273, no. 39, 1998, pages 24979 - 24982
HODSON N. ET AL., J. BIOL. CHEM., vol. 275, no. 35, 2000, pages 27311 - 27315
MCNULTY C. ET AL., MOL. MICROBIOL., vol. 59, no. 3, 2006, pages 907 - 22
JANN B.; JANN K., CURR. TOP MICROBIOL. IMMUNOL., vol. 150, 1990, pages 19 - 42
KANE T.A. ET AL., J. BIOL. CHEM., vol. 281, no. 44, 3 November 2006 (2006-11-03), pages 33192 - 33197
LINDAHL U. ET AL., J. MED. CHEM., vol. 48, no. 2, 2005, pages 349 - 352
ZHANG Z. ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 130, no. 39, 2008, pages 12998 - 13007
WANG Z. ET AL., BIOTECHNOL. BIOENG, vol. 107, no. 6, 2010, pages 964 - 973
ZANG C ET AL., METABOLIC ENGINEERING, vol. 14, no. 5, 2012, pages 521 - 527
LAIKOVA O.N. ET AL.: "Computational analysis of the transcriptional regulation of pentose utilization systems in the gamma subdivision of Proteobacteria", FEMS MICROBIOL LETT., vol. 205, no. 2, 2001, pages 315 - 22, XP027360432
BORK P. ET AL.: "Convergent evolution of similar enzymatic function on different protein folds: the hexokinase, ribokinase, and galactokinase families of sugar kinases", PROTEIN SCI., vol. 2, no. 1, 1993, pages 31 - 40
IIDA A. ET AL.: "Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12", J. BACTERIOL., vol. 158, no. 2, 1984, pages 674 - 82
PAO S.S ET AL.: "Major facilitator superfamily", MICROBIOL. MOL. BIOL. REV., vol. 62, no. 1, 1998, pages 1 - 34, XP002203285
BOYER C.; PREISS: "Biosynthesis of bacterial glycogen: Purification and properties of the Escherichia coli b alpha-1,4,-glucan: alpha-1,4-glucan 6-glycosyltansferase", J. BIOCHEMISTRY, vol. 16, no. 16, 1977, pages 3693 - 9, XP002995361, DOI: doi:10.1021/bi00635a029
DAUVILLEE D ET AL.: "Role of the Escherichia coli glgX gene in glycogen metabolism", J. BACTERIOL., vol. 187, no. 4, 2005, pages 1465 - 73
RAMANI N.: "micF antisense RNA has a major role in osmoregulation of OmpF in Escherichia coli", J. BACTERIOL., vol. 176, 1994, pages 5005 - 5010
MAJDALANI N. ET AL.: "The Rcs phosphorelay: a complex signal transduction system", ANUU. REV. MICROBIOL., vol. 59, 2005, pages 379 - 405
HOUNG H.S ET AL.: "Expression of Vi antigen in Escherichia coli K-12: characterization of ViaB from Citrobacter freundii and identity of ViaA with RcsB", J. BACTERIOL., vol. 174, 1992, pages 5910 - 5915
ROCHAPORN W. ET AL.: "Involvement of rcsB in Klebsiella K2 Capsule Synthesis in Escherichia coli K-12", J. BACTERIOL., vol. 174, 1992, pages 1063 - 1067
ANDREA RAHN ET AL.: "Transcriptional organization and regulation of The Escherichia coli", MOL. MICROBIOL., vol. 47, 2003, pages 1045 - 1060
WENDY J. KEENLEYSIDE ET AL.: "Coexpression of Colanic Acid and Serotype-Specific Capsular Polysaccharides in Escherichia coli Strains with Group II K Antigens", J. BACTERIOL., vol. 175, 1993, pages 6725 - 6730, XP000579304
LEEDS J.A.; D WELCH R.A.: "RfaH enhances elongation of Escherichia coli hlyCABD mRNA", J. BACTERIOL., vol. 178, no. 7, 1996, pages 1850 - 7, XP002472460
TEVENS M.P ET AL.: "Regulation of Escherichia coli K5 capsular polysaccharide expression: Evidence for involvement of RfaH in the expression of group II capsules", FEMS MICROBIOL. LETT., vol. 124, no. 1, 1994, pages 93 - 98
XUE P. ET AL.: "Regulation of expression of the region 3 promoter of the Escherichia coli K5 capsule gene cluster involves H-NS, SlyA, and a large 5' untranslated region", J. BACTERIOL., vol. 191, no. 6, 2009, pages 1838 - 1846
LI J. ET AL., J. BIOL. CHEM., vol. 267, no. 9, 1992, pages 6012 - 6019
LIVANIS M ET AL., J. BACTERIOL., vol. 191, no. 23, 2009, pages 7288 - 7295
BAILEY M. ET AL., MOL. MICROBIOL., vol. 22, no. 4, 1996, pages 7729 - 737
COOKSEY D.A.: "Copper uptake and resistance in bacteria", MOL. MICROBIOL., vol. 7, no. 1, 2006, pages 1 - 5
LEE J. ET AL.: "Identification of stress-related proteins in Escherichia coli using the pollutant cis-dichloroethylene", J. APPL. MICROBIOL., vol. 108, no. 6, June 2010 (2010-06-01), pages 2088 - 102
VAN DYK T.K. ET AL.: "Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?", J. BACTERIOL., vol. 186, 2004, pages 7196 - 7204
TREMPY J.E. ET AL.: "Alp suppression of Lon: dependence on the slpA gene", J. BACTERIOL., vol. 176, no. 7, 1994, pages 2061 - 7, XP000938361
HERZBERG M. ET AL.: "YdgG (TqsA) controls biofilm formation in Escherichia coli K-12 through autoinducer 2 transport", J. BACTERIOL., vol. 188, no. 2, 2006, pages 587 - 98
GUINOTE I.B. ET AL.: "Characterization of the BolA homolog IbaG: a new gene involved in acid resistance", J. MICROBIOL. BIOTECHNOL, vol. 22, no. 4, 2012, pages 484 - 93
MALINVERNI J.C.; SILHAVY T.J: "An ABC transport system that maintains lipid asymmetry in the gram-negative outer membrane", PROC. NATL. ACAD. SCI. USA, vol. 106, no. 19, 2009, pages 8009 - 14
NASEEM R ET AL.: "pH and monovalent cations regulate cytosolic free Ca(2+) in E. coli", BIOCHIM. BIOPHYS. ACTA, vol. 1778, no. 6, 2008, pages 1415 - 22, XP022673952, DOI: doi:10.1016/j.bbamem.2008.02.006
GARDNER A.M. ET AL.: "Role of NorR and sigma54 in the nitric oxide stress response", J. BIOL. CHEM., vol. 278, no. 12, 2003, pages 10081 - 6
KUZNETSOVA E. ET AL.: "Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family", J. BIOL. CHEM., vol. 281, no. 47, 2006, pages 36149 - 61, XP055038714, DOI: doi:10.1074/jbc.M605449200
THOMPSON K.M. ET AL.: "SigmaE regulates and is regulated by a small RNA in Escherichia coli", J. BACTERIOL., vol. 189, no. 11, 2007, pages 4243 - 56
JOHANSEN J. ET AL.: "Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteins", J. MOL. BIOL., vol. 364, no. 1, 2006, pages 1 - 8
ANANTHARAMAN V ET AL.: "SPOUT: a class of methyltransferases that includes spoU and trmD RNA methylase superfamilies, and novel superfamilies of predicted prokaryotic RNA methylases", J. MOL. MICROBIOL. BIOTECHNOL., vol. 4, no. 1, 2002, pages 71 - 5
BURR B. ET AL.: "Homoserine kinase from Escherichia coli K12", EUR. J. BIOCHEM., vol. 62, no. 3, 1976, pages 519 - 26
CLARK R.B.; OGILVIE J.W. ET AL.: "Aspartokinase I-homoserine dehydrogenase I of Escherichia coli K12: Subunit molecular weight and nicotinamide-adenine dinucleotide phosphate binding", BIOCHEMISTRY, vol. 11, no. 7, 1972, pages 1278 - 82
LYNN S.P. ET AL.: "Attenuation regulation in the thr operon of Escherichia coli K-12: molecular cloning and transcription of the controlling region", J. BACTERIOL., vol. 152, no. L, 1982, pages 363 - 71
PRIOR T.I.; KORNBERG H.L.: "Nucleotide sequence of fruA, the gene specifying enzyme IIfru of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Escherichia coli K12", J. GEN. MICROBIOL., vol. 134, no. 10, 1988, pages 2757 - 68, XP002937888
SOLOMON L.R; BREITMAN T.R: "Pseudouridine kinase of Escherichia coli: a new enzyme", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 44, no. 2, 1971, pages 299 - 304, XP024778957, DOI: doi:10.1016/0006-291X(71)90599-7
FRAENKEL D.G; HORECKER B.L.: "Fructose-1,6-diphosphatase and acid hexose phosphatase of Escherichia coli", J. BACTERIOL., vol. 90, no. 4, 1965, pages 837 - 42
NEUMANN M. ET AL.: "A periplasmic aldehyde oxidoreductase represents the first molybdopterin cytosine dinucleotide cofactor containing molybdo-flavoenzyme from Escherichia coli", FEBS J., vol. 276, no. 10, 2009, pages 2762 - 74
MOUSSATOVA A. ET AL.: "ATP-binding cassette transporters in Escherichia coli", BIOCHIM. BIOPHYS. ACTA, vol. 1778, no. 9, 2008, pages 1757 - 71, XP025408804, DOI: doi:10.1016/j.bbamem.2008.06.009
BOEHM A ET AL.: "Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress", MOL. MICROBIOL., vol. 72, no. 6, 2009, pages 1500 - 16
PELLUDAT C ET AL.: "The yersiniabactin biosynthetic gene cluster of Yersinia enterocolitica: organization and siderophore-dependent regulation", J. BACTERIOL., vol. 180, no. 3, 1998, pages 538 - 46
ZHANG X.S. ET AL.: "YcfR (BhsA) influences Escherichia coli biofilm formation through stress response and surface hydrophobicity", J. BACTERIOL., vol. 189, no. 8, 2007, pages 3051 - 62
MAGNET S. ET AL.: "Identification of the L,D-transpeptidases responsible for attachment of the Braun lipoprotein to Escherichia coli peptidoglycan", J. BACTERIOL., vol. 189, no. 10, 2007, pages 3927 - 31
DALBEY R.E.: "Leader peptidase", MOL. MICROBIOL., vol. 5, no. 12, 1991, pages 2855 - 60
ROBERTSON H.D; DUNN J.J.: "Ribonucleic acid processing activity of Escherichia coli ribonuclease III", J. BIOL. CHEM., vol. 250, no. 8, 25 December 1974 (1974-12-25), pages 3050 - 6
TAKIFF H.E ET AL.: "Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon", J. BACTERIOL., vol. 174, no. 5, 1992, pages 1544 - 53, XP000673194
ZHANG J.; INOUYE M.: "MazG, a nucleoside triphosphate pyrophosphohydrolase, interacts with Era, an essential GTPase in Escherichia coli", J. BACTERIOL., vol. 184, no. 19, 2002, pages 5323 - 9
LABER B ET AL.: "Escherichia coli dihydrodipicolinate synthase: Identification of the active site and crystallization", BIOCHEM. J., vol. 288, 1992, pages 691 - 5, XP009035909
GHRIST A.C. ET AL.: "GcvR interacts with GcvA to inhibit activation of the Escherichia coli glycine cleavage operon", MICROBIOLOGY, vol. 147, 2001, pages 2215 - 21
CLARKE D.J. ET AL.: "Interrogating the molecular details of the peroxiredoxin activity of the Escherichia coli bacterioferritin comigratory protein using high-resolution mass spectrometry", BIOCHEMISTRY, vol. 48, no. 18, 2009, pages 3904 - 14
ANDREWS S.C. ET AL.: "A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system", MICROBIOLOGY, vol. 143, 1997, pages 3633 - 47
ADES S.E. ET AL.: "Regulation of the alternative sigma factor sigma(E) during initiation, adaptation, and shutoff of the extracytoplasmic heat shock response in Escherichia coli", J. BACTERIOL., vol. 185, no. 8, 2003, pages 2512 - 9
MORTARINO M ET AL.: "L-aspartate oxidase from Escherichia coli, I. Characterization of coenzyme binding and product inhibition", EUR. J. BIOCHEM., vol. 239, no. 2, 1996, pages 418 - 26, XP055153570, DOI: doi:10.1111/j.1432-1033.1996.0418u.x
GOLOVINA A.Y. ET AL., THE YFIC GENE OF E. COLI ENCODES AN ADENINE-N6 METHYLTRANSFERASE THAT SPECIFICALLY MODIFIES A37 OF TRNALVAL(CMO5UAC, vol. 15, no. 6, 2009, pages 1134 - 41
CHAROLLAIS J. ET AL.: "The DEAD-box RNA helicase SrmB is involved in the assembly of 50S ribosomal subunits in Escherichia coli", MOL. MICROBIOL., vol. 48, no. 5, 2003, pages 1253 - 65
BRAUN M ET AL.: "Characterization of the overproduced NADH dehydrogenase fragment of the NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli", BIOCHEMISTRY, vol. 37, no. 7, 1998, pages 1861 - 7
KALAMORZ F. ET AL.: "Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli", MOL. MICROBIOL., vol. 65, no. 6, 2007, pages 1518 - 33
URBAN J.H.; VOGEL J. ET AL.: "Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation", PLOS BIOL., vol. 6, no. 3, 2008, pages E64
DAILEY T.A. ET AL.: "Expression of a cloned protoporphyrinogen oxidase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, 1994, pages 813 - 815
SASARMAN A ET AL.: "Nucleotide sequence of the hemX gene, the third member of the Uro operon of Escherichia coli K12", NUCLEIC ACIDS RES., vol. 16, no. 24, 1988, pages 11835
JORDAN P.M.; WOODCOCK S.C.: "Mutagenesis of arginine residues in the catalytic cleft of Escherichia coli porphobilinogen deaminase that affects dipyrromethane cofactor assembly and tetrapyrrole chain initiation and elongation", BIOCHEM. J., vol. 280, 1991, pages 445 - 9
KIMURA S ET AL.: "Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity", NUCLEIC ACIDS RES., vol. 40, no. 9, 2012, pages 4071 - 85
LINTON K.J.; HIGGINS C.F.: "The Escherichia coli ATP-binding cassette (ABC) proteins", MOL. MICROBIOL., vol. 28, no. 1, 1998, pages 5 - 13
MADSEN C.T. ET AL.: "Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry", NUCLEIC ACIDS RES., vol. 31, no. 16, 2003, pages 4738 - 46
RAPP M. ET AL.: "Experimentally based topology models for E. coli inner membrane proteins", PROTEIN SCI., vol. 13, no. 4, 2004, pages 937 - 45
HENDERSON I.R; OWEN P.: "The major phase-variable outer membrane protein of Escherichia coli structurally resembles the immunoglobulin A1 protease class of exported protein and is regulated by a novel mechanism involving Dam and oxyR", J. BACTERIOL., vol. 181, no. 7, 1999, pages 2132 - 41
MACIAG A ET AL.: "In vitro transcription profiling of the cyS subunit of bacterial RNA polymerase: Re-definition of the ?S regulon and identification of oS-specific promoter sequence elements", NUCLEIC ACIDS RES., vol. 39, no. 13, 2011, pages 5338 - 55
PEEKHAUS N. ET AL.: "Characterization of a novel transporter family that includes multiple Escherichia coli gluconate transporters and their homologues", FEMS MICROBIOL. LETT., vol. 147, no. 2, 1997, pages 233 - 8, XP002204792, DOI: doi:10.1016/S0378-1097(96)00532-0
WASSARMAN K.M. ET AL.: "Identification of novel small RNAs using comparative genomics and microarrays", GENES, DEV., vol. 15, no. 13, 2001, pages 1637 - 51, XP002223289, DOI: doi:10.1101/gad.901001
GU M.; IMLAY J.A.: "The SoxRS response of Escherichia coli is directly activated by redox-cycling drugs rather than by superoxide", MOL. MICROBIOL., vol. 79, no. 5, 2011, pages 1136 - 50
TOUATI D.: "Sensing and protecting against superoxide stress in Escherichia coli -- how many ways are there to trigger soxRS response?", REDOX REP., vol. 5, no. 5, 2000, pages 287 - 93
LEE J.H ET AL.: "SoxRS-mediated lipopolysaccharide modification enhances resistance against multiple drugs in Escherichia coli", J. BACTERIOL., vol. 191, no. 13, 2009, pages 4441 - 50
BOEHM A ET AL.: "Second messenger signaling governs Escherichia coli biofilm induction upon ribosomal stress", MOL. MICROBIOL., vol. 72, no. 6, 2009, pages 1500 - 16
CAO J. ET AL.: "EfeUOB (YcdNOB) is a tripartite, acid-induced and CpxAR-regulated, low-pH Fe2+ transporter that iscryptic in Escherichia coli K-12 but functional in E. coli 0-157:H7", MOL. MICROBIOL., vol. 65, 2007, pages 857 - 875
BACKMANN B.J.: "Escherichia coli and Salmonella Cellular and Molecular Biology", 1996, AMERICAN SOCIETY FOR MICROBIOLOGY PRESS, article "Derivations and Genotypes of some mutant derivatives of Escherichia coli K-12", pages: 2460 - 2488
CADWELL, R.C., PCR METH. APPL., vol. 2, 1992, pages 28
STEMMER, W.P., NATURE, vol. 370, 1994, pages 389
ZHAO, H., NATURE BIOTECHNOL., vol. 16, 1998, pages 258
MILLER, J.H.: "Experiments in Molecular Genetics", 1972, COLD SPRING HARBOR LABORATORY
DATSENKO, K.A.; WANNER, B.L: "Proc. Natl. Acad. Sci. USA", vol. 97, 2000, pages: 6640 - 6645
"Genetic Engineering", vol. 8, 1987, KYORITSU SHUPPAN CO., LTD, article "Fundamental Microbiology"
GENE, vol. 60, no. 1, 1987, pages 115 - 127
GOLDSTEIN ET AL.: "Prokaryotic Promoters in Biotechnology", BIOTECHNOL. ANNU. REV., vol. 1, 1995, pages 105 - 128, XP008038474
OLINS P.O. ET AL., GENE, vol. 73, 1988, pages 227 - 235
KANE, J.F., CURR. OPIN. BIOTECHNOL., vol. 6, no. 5, 1995, pages 494 - 500
HIGUCHI, R.: "PCR Technology", vol. 61, 1989, STOCKTON PRESS
CARTER, P., METH. IN ENZYMOL., vol. 154, 1987, pages 382
KRAMER, W.; FRITS, H.J., METH. IN ENZYMOL, vol. 154, 1987, pages 350
KUNKEL, T.A. ET AL., METH. IN ENZYMOL., vol. 154, 1987, pages 367
NAKAMURA, Y ET AL., NUCL. ACIDS RES., vol. 28, 2000, pages 292
MANDEL, M.; HIGA, A., J. MOL. BIOL., vol. 53, 1970, pages 159 - 162
DUNCAN, C.H.; WILSON, G.A; YOUNG, F.E., GENE, vol. 1, 1977, pages 153 - 167
CHANG, S; CHOEN, S.N., MOL. GEN. GENET., vol. 168, 1979, pages 111 - 115
BIBB, M.J.; WARD, J.M; HOPWOOD, O.A., NATURE, vol. 274, 1978, pages 398 - 400
HINNEN, A.; HICKS, J.B.; FINK, G.R., PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929 - 1933
SAMBROOK, J. ET AL.: "Molecular Cloning A Laboratory Manual, 3rd ed.", 2001, COLD SPRING HARBOR LABORATORY PRESS
"Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
BITTER T.; MUIR H.M.: "A modified uronic acid carbazole reaction", ANALYTICAL BIOCHEMISTRY, vol. 4, no. 4, 1962, pages 330 - 334, XP024827447, DOI: doi:10.1016/0003-2697(62)90095-7
ZHANG Z. ET AL.: "Solution Structures of Chemoenzymatically Synthesized Heparin and Its Precursors", J. AM. CHEM. SOC., vol. 130, no. 39, 2008, pages 12998 - 13007, XP055237054, DOI: doi:10.1021/ja8026345
KUBERAN B. ET AL.: "Chemoenzymatic Synthesis of Classical and Non-classical Anticoagulant Heparan Sulfate Polysaccharides", J. BIOL. CHEM., vol. 278, 2003, pages 52613 - 52621
KUBERAN B. ET AL.: "Chemoenzymatic Synthesis of Classical and Non-classical Anticoagulant Heparan Sulfate Polysaccharides", J. BIOL. CHEM., vol. 278, no. 52, 2003, pages 52613 - 52621
CHEN J. ET AL.: "Enzymatic redesigning of biologically active heparan sulfate", J. BIOL. CHEM., vol. 280, no. 52, 30 December 2005 (2005-12-30), pages 42817 - 25, XP002609045, DOI: doi:10.1074/JBC.M504338200
BITTER, T.; MURIR H.M., ANAL. BIOCHEM., vol. 4, 1962, pages 330 - 334
AMANN E. ET AL.: "Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli", GENE, vol. 25, no. 2-3, 1983, pages 167 - 78, XP023599405, DOI: doi:10.1016/0378-1119(83)90222-6
DATSENKO; WANNER: "One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products", PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 6645, XP002210218, DOI: doi:10.1073/pnas.120163297
DATSENKO; WANNER, PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 45
SAMBROOK, J. ET AL.: "Molecular Cloning A Laboratory Manual, 2nd ed.", 1989, COLD SPRING HARBOR LABORATORY PRESS
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