Description

HEPATIΗS C VIRUS RIBOZYMES

Technical Field

The present invention relates generally to ribozymes, and more specifically, to ribozymes which are capable of cleaving Hepatitis C Virus nucleic acids, as well as to methods which utilize such ribozymes.

Background of the Invention

Hepatitis C Virus ("HCV") is an RNA virus which is responsible for approximately 75% of all cases of non-A, non-B hepatitis. Based upon epidemiologic and serologic surveys, it has been estimated that at least 1% to 2% of the world population is chronically infected with HCV (Davis et al., "Therapy for Chronic Hepatitis C" in Gastroenterology Clinics of North America, pp. 603-613, 1995). In the United States, approximately 150,000 acute cases occur annually, where it is the ninth leading cause of death. Moreover, approximately 50% of the acutely infected individuals go on to develop chronic liver disease, and of these, 25% will develop cirrhosis. In addition, on a worldwide basis, 50% of the cases of hepatocellular carcinoma are correlated with HCV infection.

HCV is a positive-stranded RNA virus that is related to the flaviviridae family. The virus, which was isolated and characterized in 1989 (Choo et al., Science 244:362-364, 1989) has an -9.5 Kb linear genome that replicates through a double stranded RNA intermediate, mediated by a virally encoded RNA-dependent RNA polymerase. There is no known DNA intermediate in the replication process. Presumably, because its replication is exclusively through RNA polymerases, which lack proofreading activities, variability in the coding sequences is a hallmark of individual HCV isolates.

At present, the only therapy which shows some promise for the treatment acute and chronic cases of HCV is alpha interferon (Fried and Hoofhagle, Seminars in Liver Disease 75(1):82-91, 1995). Treatment with alpha interferon however, particularly for chronic patients, produces only temporary results. In particular, in most studies where patients with chronic HCV infections are treated with alpha interferon, only 20% to 25% maintain a sustained, long-term response (Fried and Hoofhagle, supra). In addition, treatment with alpha interferon can produce a wide array of side effects, including systemic effects (e.g., fatigue, fever, headache, anorexia, weight loss,

nausea, vomiting, diarrhea, and hair loss), neurologic and psychological effects, an increased susceptibility to infections, as well as an assortment of autoimmune diseases.

The present invention provides an effective treatment to combat HCV infection, and further provides other, related advantages.

Summary of the Invention

The present invention provides ribozymes useful to treat or prevent Hepatitis C Virus ("HCV") infection or disease in an organism or subject, as well as methods of treating HCV infection or disease. Reagents such as vectors, host cells, DNA molecules coding for these ribozymes useful in methods of treatment and prevention of HCV infection or disease also are provided.

Accordingly, in one aspect the present invention ribozymes are provided which have the ability to inhibit replication, infectivity, or gene expression of a hepatitis C virus. Within certain embodiments, the ribozyme is a hammerhead or hairpin ribozyme. Within other embodiments, the ribozyme cleaves genomic strand RNA (either the positive or negative strand), representative examples of which include the sequences set forth in Table I, below. In other aspects, the present invention also provides nucleic acid molecules encoding such ribozymes where, within certain embodiments the nucleic acid molecule is DNA or cDNA. Within preferred embodiments, the nucleic acid molecule is under the control of a promoter to transcribe the nucleic acid molecule.

In another aspect, the present invention provides host cells containing the ribozymes described herein, vectors comprising a promoter operatively linked to the nucleic acid molecule which encodes the ribozymes described herein, and host cells containing such vectors. Within certain embodiments, the vector is a plasmid, a viral vector, retrotransposon, or a cosmid. Representative examples of promoters include the polIII and CMV promoters.

In a further aspect, the present invention provides methods for producing a ribozyme, the ribozyme being able to inhibit hepatitis C viral infection and replication in a cell, comprising the step of providing a nucleic acid molecule (e.g., DNA) encoding a ribozyme under the transcriptional control of a promoter (e.g., in a vector), and transcribing the nucleic acid molecule to produce the ribozyme. The method may also further comprise purifying the ribozyme so produced. The ribozyme may be produced in vitro, in vivo or ex vivo. In yet another aspect, the present invention provides methods of interfering with or preventing HCV replication in a cell infected with HCV, comprising

the step of introducing into a cell an effective amount of the ribozymes described herein. In one embodiment, such methods comprise introducing into the cell an effective amount of DNA encoding a ribozyme as described herein and transcribing the DNA to produce the ribozyme. In still a further aspect, the present invention provides methods of preventing hepatitis C viral infection in a cell susceptible to infection with HCV, comprising the step of introducing into the cell an effective amount of a nucleic acid molecule (e.g., DNA) encoding a ribozyme as described herein and transcribing the DNA to produce the ribozyme. In preferred embodiments, the methods further comprise administering the cell transduced with a retroviral vector to a mammal of the same species as that from which the transduced cell was obtained. In other preferred embodiments, the cell transduced with the retroviral vector has been obtained from the mammal receiving the transduced cell. The above-described methods, as well as the compositions described herein, may be utilized to treat or prevent HCV infection or disease in a wide variety of warm-blooded animals or mammals, including for example, humans.

These and other aspects of the present invention will become evident upon reference to the following detailed description and attached drawings. In addition, various references are set forth herein that describe in more detail certain procedures or compositions (e.g., plasmids, etc.), and are therefore incoφorated by reference in their entirety as if each were individually noted for incoφoration.

Brief Description of the Drawings Figure 1 is a schematic illustration of vector pGem7Z (Promega,

Madison, WI).

Figure 2 is a schematic illustration of vector pLNT-Rz.

Figure 3 is a schematic illustration of one representative haiφin ribozyme (Seq. ID Nos. 70 and 71) and one tetraloop haiφin ribozyme (CR4) (Seq. ID Nos. 73 and 71).

Figure 4 is an in vitro cleavage timecourse of CR2 ribozyme cleaving a short 5 'UTR substrate.

Figure 5 is a graph which depicts in vitro cleavage timecourse reactions using CR2 and various facilitator RNA molecules, cleaving the long 5'UTR substrate.

Figure 6 depicts two in vitro cleavage experiments, one with and one without a pre-heating step of 90°C for 1 minute prior to the cleavage reaction. Ribozymes tested are CR2, CR6, CR7 and CR8 using the short 5'UTR substrate.

Figure 7 shows in vitro cleavage reactions using variants of CR4 ribozyme with either 8, 7 or 6 nucleotides in Helix 1, using the short capsid substrate.

Figure 8 depicts in vitro cleavage reactions using variants of CR4 ribozyme with either 8, 7 or 6 nucleotides in Helix 1 using the long capsid substrate.

Figures 9A, 9B, 9C and 9D are schematic illustrations of certain vectors described herein: pPur, pPur-HCV, pLNL6 and pLNL-Pur-HCV. Figure 10 is an RNase protection assay verifying ribozyme expression after transfection.

Figures 1 1 A, 11B and 1 IC are three graphs which show ribozyme cleavage obtained in co-transfection experiments.

Figure 12 is a schematic illustration of the experimental design used to test the protective effect of the ribozymes.

Figure 13 is a graph showing ribozyme cleavage and subsequent protection of cells from incoming HCV -containing positive-stranded RNA virus.

Figure 14 is a western blot indicating detection of HCV nucleocapsid protein. Figure 15 is a schematic illustration of vector pGL3 (Promega, Madison,

WI).

Figure 16 is a schematic illustration of adenoviral vectors expressing either β-galactosidase or ribozyme.

Figure 17 is a graph which shows FPLC data generated during purification of adenoviral vectors from cell lysates.

Figure 18 is a schematic illustration of an AAV ribozyme expression vector.

Figure 19 is an autorad which shows the results of in vitro cleavage of short (<50 nucleotides) substrates by two ribozymes (CNR3 and CNR6) against the (-) strand of HCV.

Figure 20 is an autorad which shows the results of in vitro cleavage of long substrates by two ribozymes (CNR3 and CNR6) against the (-) strand of HCV.

Figure 21 is a schematic illustration of vectors pLNT-Rz, pAvC-Rz, pAvM-Rz, pAvCM-Rz and pAAVM-Rz. Figure 22 is a schematic illustration of pAMFT.dBAM.

Figure 23 is a schematic illustration of vectors pPur-HCV, pLNL-PUR- HCV and pGem4-HCV 5'C.

Figure 24 is a western blot which shows expression of HCV core protein following transfection into HT1080 cells. Figure 25A is a blot which shows the ability of different promoter/vector combinations to reduce viral core expression in cell culture. pAAVM-CR4 shows the greatest efficacy. Figure 25B is a blot which shows the further titration of pAAVM- CR4 and its effect on viral core expression.

Figure 26 shows the results of an RNase protection assay performed to measure expression of ribozyme CR4 in human liver cells.

Figure 27 is a table which compares the infectivity of primary normal human hepatocytes with either recombinant adenovirus or recombinant adeno- associated virus carrying the β-galactosidase gene.

Detailed Description of the Invention

DEFINITIONS Prior to setting forth the invention, it may be helpful to an understanding thereof to first set forth definitions of certain terms that will be used hereinafter.

"Ribozyme" refers to a nucleic acid molecule which is capable of cleaving a specific nucleic acid sequence. Ribozymes may be composed of RNA, DNA, nucleic acid analogues (e.g., phosphorothioates), or any combination of these (e.g., DNA/RNA chimerics). Within particularly preferred embodiments, a ribozyme should be understood to refer to RNA molecules that contain anti-sense sequences for specific recognition, and an RNA-cleaving enzymatic activity. "Ribozyme gene" refers to a nucleic acid molecule (e.g., DNA) consisting of the ribozyme sequence which, when transcribed into RNA, will yield the ribozyme.

"Vector" refers to an assembly which is capable of expressing a ribozyme of interest. The vector may be composed of either deoxyribonucleic acids ("DNA") or ribonucleic acids ("RNA"). Optionally, the vector may include a polyadenylation sequence, one or more restriction sites, as well as one or more selectable markers such as neomycin phosphotransferase, hygromycin phosphotransferase or puromycin-N-acetyl-transferase. Additionally, depending on the host cell chosen and the vector employed, other genetic elements such as an origin of replication, additional nucleic acid restriction sites, enhancers, sequences conferring

<o inducibility of transcription, and selectable markers, may also be incoφorated into the vectors described herein.

As noted above, the present invention provides ribozymes which are capable of cleaving Hepatitis C Virus nucleic acids. Briefly, the viral genome of HCV has an approximately 9.5 Kb linear genome that replicates through a double stranded RNA intermediate (Choo et al., Proc. Natl. Acad. Sci. USA #5:2451-2455, 1991; Choo et al., Brit. Med. Bull. 4<5(2):423-441, 1990; Okamoto et al., J. Gen. Vir. 72:2697-2704, 1991; see also, e.g., Genbank Accession No. M67463, Intelligenetics (Mountain View, California). This sequence expresses a polyprotein precursor of 3011 amino acids, which is cleaved to yield several different viral proteins, including C (nucleocapsid protein) El, E2 NS1, and non-structural proteins NS2, NS3, NS4, and NS5 (Houghton et al., Hepatology 74:381-388, 1991).

Sequence analyses of HCV isolates indicate considerable sequence variation (for reviews see Houghton et al., Hepatology 4:381-388, 1991; van Doom, J. Med. Virology 43:345-356, 1994; Bukh et al., Seminars in Liver Disease 75:41-63, 1995; Simmonds, Hepatology 27:570-583, 1995; see also Genbank Assession Nos. D10749, D10750, D11168, D11355, D13558, D30613, D90208, L02836, M58335, M84754, M96362, S62220, U01214, U16362, X61596). The most conserved regions (>90% sequence identity within the known isolates) reside within the first 1000 nucleotides of the genome, which consists of a 5' untranslated region (5 'UTR) and the coding region for the nucleocapsid (Bukh et al., Proc. Natl. Acad. Sci. USA 89:4942- 4946, 1992). The highest degree of sequence variability (up to 50% sequence divergence) is found within the El and NS2 genes (see above reviews). Based on this sequence heterogeneity, HCV isolates can be categorized into at least 12 distinct genotypes (Okamoto et al., Virology 188:331-341, 1992; Bukh, et al., Proc. Natl. Acad. Sci. USA 90:8234-8238, 1993). Although most of the HCV genotypes are distributed worldwide, certain strains are found in discrete geographical regions (Bukh, et al., Proc. Natl. Acad. Sci. USA 90:8234-8238, 1993) and their distribution may play a role in ribozyme-mediated therapy.

RIBOZYMES

As noted above, the present invention provides ribozymes having the ability to inhibit replication, infectivity, or gene expression of a hepatitis C viral infection in a cell. Several different types of ribozymes may be constructed for use within the present invention, including for example, hammerhead ribozymes (Rossi, J.J.

et al., Pharmac. Ther. 50:245-254, 1991) (Forster and Symons, Cell 45:211-220, 1987; Haseloff and Gerlach, Nature 328:596-600, 1988; Walbot and Bruening, Nature 334:196, 1988; Haseloff and Gerlach, Nature 354:585, 1988; Haseloff et al., U.S. Patent No. 5,254,678), haiφin ribozymes (Hampel et al, Nucl. Acids Res. 75:299-304, 1990, and U.S. Patent No. 5,254,678), hepatitis delta virus ribozymes (Perrotta and Been, Biochem. 37:16, 1992), Group I intron ribozymes (Cech et al., U.S. Patent No. 4,987,071) and RNase P ribozymes (Takada et al., Cell 35:849, 1983); (see also, WO 95/29241, entitled "Ribozymes with Product Ejection by Strand Displacement"; and WO 95/31551, entitled "Novel Enzymatic RNA Molecules." Cech et al. (U.S. Patent No. 4,987,071, issued January 22, 1991) disclosed the preparation and use of ribozymes which are based on the properties of the Tetrahymena ribosomal RNA self-splicing reaction. These ribozymes require an eight base pair target site and free guanosine (or guanosine derivatives). A temperature optimum of 50°C is reported for the endoribonuclease activity. The fragments that arise from cleavage contain 5'-phosphate and 3'-hydroxyl groups and a free guanosine nucleotide added to the 5'-end of the cleaved RNA.

In contrast to the ribozymes of Cech et al., particularly preferred ribozymes of the present invention hybridize efficiently to target sequences at physiological temperatures, making them suitable for use in vivo, and not merely as research tools (see column 15, lines 18 to 42, of Cech et al., U.S. Patent No. 4,987,071 ). Thus, particularly preferred ribozymes for use within the present invention include haiφin ribozymes (for example, as described by Hampel et al., European Patent Publication No. 0 360 257, published March 26, 1990) and hammerhead ribozymes. Briefly, the sequence requirement for the haiφin ribozyme is any RNA sequence consisting of NNNBN*GUC(N) _X (Sequence ID Nos. 1-5) (where x is any number from 6 to 10, N*G is the cleavage site, B is any of G, C, or U, and N is any of G, U, C, or A). Representative examples of recognition or target sequences for haiφin ribozymes are set forth below in the Examples. Additionally, the backbone or common region of the haiφin ribozyme can be designed using the nucleotide sequence of the native haiφin ribozyme (Hampel et al., Nucl. Acids Res. 75:299-304, 1990) or it can be modified to include a "tetraloop" structure that increases stability and catalytic activity (see Example 2 and Figure 3; see also Yu et al., Virology 206:381-386, 1995; Cheong et al., Nature 346:680-682, 1990; Anderson et al., Nucl. Acids Res. 22:1096-1100, 1994).

The sequence requirement at the cleavage site for the hammerhead ribozyme is any RNA sequence consisting of NUX (where N is any of G, U, C, or A and X represents C, U, or A) can be targeted. Accordingly, the same target within the

haiφin leader sequence, GUC, is useful for the hammerhead ribozyme. Additional nucleotides of the hammerhead ribozyme or haiφin ribozyme are determined by the target flanking nucleotides and the hammerhead consensus sequence (see Ruffher et al., Biochemistry 29:10695-10702, 1990). This information, along with the sequences and disclosure provided herein, enables the production of haiφin ribozymes of this invention. Appropriate base changes in the ribozyme are made to maintain the necessary base pairing with the target HCV sequences.

The ribozymes of this invention, as well as DNA encoding such ribozymes and other suitable nucleic acid molecules, described in more detail below, can be chemically synthesized using methods well known in the art for the synthesis of nucleic acid molecules (see e.g., Heidenreich et al., J. FASEB 70(l):90-6, 1993; Sproat, Curr. Opin. Biotechnol. 4(l):20-28, 1993). Alternatively, commercial suppliers such as Promega, Madison, Wis., USA, provide a series of protocols suitable for the production of nucleic acid molecules such as ribozymes. Within one aspect of the present invention, ribozymes are prepared from a DNA molecule or other nucleic acid molecule (which, upon transcription, yields an RNA molecule) operably linked to an RNA polymerase promoter, e.g., the promoter for T7 RNA polymerase or SP6 RNA polymerase. Accordingly, also provided by this invention are nucleic acid molecules, e.g., DNA or cDNA, coding for the ribozymes of this invention. When the vector also contains an RNA polymerase promoter operably linked to the DNA molecule, the ribozyme can be produced in vitro upon incubation with the RNA polymerase and appropriate nucleotides. In a separate embodiment, the DNA may be inserted into an expression cassette, such as described in Cotten and Birnstiel, EMBO J. 5(12):3861-3866, 1989, and in Hempel et al., Biochemistry 25:4929-4933, 1989. A more detailed discussion of molecular biology methodology is disclosed in Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, 1989.

During synthesis, the ribozyme can be modified by ligation to a DNA molecule having the ability to stabilize the ribozyme and make it resistant to RNase (Rossi et al., Pharmac. Ther. 50:245-254, 1991). Alternatively, the ribozyme can be modified to a phosphothio-analog for use in liposome delivery systems. This modification also renders the ribozyme resistant to endonuclease activity.

VECTORS Use of ribozymes to treat HCV infection involves introduction of functional ribozyme to the infected cell of interest. This can be accomplished by either synthesizing functional ribozyme in vitro prior to delivery, or, by delivery of DNA capable of driving ribozyme synthesis in vivo.

More specifically, within other aspects of the invention the ribozyme gene may be constructed within a vector which is suitable for introduction to a host cell (e.g., prokaryotic or eukaryotic cells in culture or in the cells of an organism). Appropriate prokaryotic and eukaryotic cells can be transfected with an appropriate transfer vector containing the nucleic acid molecule encoding a ribozyme of this invention.

To produce the ribozymes with a vector in vivo, nucleotide sequences coding for ribozymes are preferably placed under the control of a eukaryotic promoter such as pol III (e.g., tRNA or VA-1 from adenovirus), CMV, SV40 late, or SV40 early promoters. Within certain embodiments, the promoter may be a tissue-specific promoter such as, for example, a liver-specific promoter such as the albumin promoter and the alphafetoprotein promoter (Feuerman et al., Mol. Cell. Biol. 9:4204-12, 1989; Camper and Tilghman, Genes Develop. 3:537-46, 1989); the alcohol dehydrogenase promoter (Felder, Proc. Natl. Acad. Sci. USA 56:5903-07, 1989); the Apolipoprotein B gene promoter (Das et al., J. Biol. Chem. 263Λ 1452-8, 1988); the Coagulation protease factor VII gene promoter (Erdmann et al., J. Biol. Chem. 270:22988-96, 1995); the Fibrinogen gamma gene promoter (Zhang et al., J Biol. Chem. 270:24287-91, 1995); the Glucokinase gene promoter (Williams et al., Biochem. Biophys., Res. Comm. 272:272-9, 1995); the Liver phosphofructokinase gene promoter (Levanon et al., Biochem. Mol. Biol. Int. 35:729-36, 1995); the Phospho-Enol-Pyruvate Carboxy-Kinase ("PEPCK") promoter (Hatzogiou et al., J Biol. Chem. 263: 17798-808, 1988; Benvenisty et al., Proc. Natl. Acad. Sci. USA 56:1118-22, 1989; Vaulont et al., Mol. Cell. Biol. 9:4409-15, 1989); or lymphoid-specific promoters. Ribozymes may thus be produced directly from the transfer vector in vivo. A wide variety of vectors may be utilized within the context of the present invention, including for example, plasmids, viruses, retrotransposons and cosmids. Because HCV is an acute or chronic infection of the liver, vectors with hepatotrophic properties are particularly preferred. Representative examples include adenoviral vectors (e.g., WO 94/26914, WO 93/9191 ; Yei et al., Gene Therapy 7:192- 200, 1994; Kolls et al., PNAS 97(1):215-219, 1994; Kass-Eisler et al., PNAS 90(24): 11498-502, 1993; Guzman et al., Circulation 55(6):2838-48, 1993; Guzman et

al., Cir. Res. 73(6): 1202- 1207, 1993; Zabner et al., Cell 75(2):207-216, 1993; Li et al., Hum Gene Ther. 4(4):403-409, 1993; Caillaud et al., Eur. J. Neurosci. 5(10): 1287- 1291, 1993), adeno-associated type 1 ("AAV-1 ") or adeno-associated type 2 ("AAV-2") vectors (see WO 95/13365; Flotte et al., PNAS 90(22): 10613-10617, 1993), hepatitis delta vectors, live, attenuated delta viruses and heφes viral vectors (e.g., U.S. Patent No. 5,288,641), as well as vectors which are disclosed within U.S. Patent No. 5,166,320. Other representative vectors include retroviral vectors (e.g., EP 0 415 731; WO 90/07936; WO 91/02805; WO 94/03622; WO 93/25698; WO 93/25234; U.S. Patent No. 5,219,740; WO 93/11230; WO 93/10218). For vectors without inherent hepatotropism (e.g., AAV or retroviruses), cell tropism can be altered to specifically target these viruses to the liver. Methods of using such vectors in gene therapy are well known in the art, see, for example, Larrick, J.W. and Burck, K.L., Gene Therapy: Application of Molecular Biology, Elsevier Science Publishing Co., Inc., New York, New York, 1991 and Kreigler, M., Gene Transfer and Expression: A Laboratory Manual, W.H. Freeman and Company, New York, 1990.

Further provided by this invention are vectors having more than one nucleic acid molecule encoding a ribozyme of this invention, each molecule under the control of a separate eukaryotic promoter (or, an Internal Ribosome Entry Site or "IRES"), or alternatively, under the control of single eukaryotic promoter. Representative examples of other nucleic acid molecules which may be delivered by the vectors of the present invention include therapeutic molecules such as interferon (e.g., alpha, beta or gamma), as well as a wide variety of other cytokines or growth factors, and facilitators which assist or aid ribozymes in cleaving a target sequence by unwinding or otherwise limiting secondary folding which might otherwise inhibit the ribozyme (see Example 4). These vectors provide the advantage of providing multi¬ functional therapy against HCV infection, preferably with the various therapies working together in synergy.

Host prokaryotic and eukaryotic cells stably harboring the vectors described above also are provided by this invention. Suitable host cells include bacterial cells, rat cells, mouse cells, and human cells, for example, liver and blood cells.

DELIVERY Within certain aspects of the invention, ribozyme molecules, or nucleic acid molecules which encode the ribozyme, may be introduced into a host cell utilizing

II a vehicle, or by various physical methods. Representative examples of such methods include transformation using calcium phosphate precipitation (Dubensky et al., PNAS 57:7529-7533, 1984), direct microinjection of such nucleic acid molecules into intact target cells (Acsadi et al., Nature 352:815-818, 1991), and electroporatiόn whereby cells suspended in a conducting solution are subjected to an intense electric field in order to transiently polarize the membrane, allowing entry of the nucleic acid molecules. Other procedures include the use of nucleic acid molecules linked to an inactive adenovirus (Cotton et al., PNAS 59:6094, 1990), lipofection (Feigner et al., Proc. Nail. Acad. Sci. USA 54:7413-7417, 1989), microprojectile bombardment (Williams et al., PNAS 55:2726-2730, 1991), polycation compounds such as polylysine, receptor specific ligands, liposomes entrapping the nucleic acid molecules, spheroplast fusion whereby E. coli containing the nucleic acid molecules are stripped of their outer cell walls and fused to animal cells using polyethylene glycol, viral transduction, (Cline et al., Pharmac. Ther. 29:69, 1985; and Friedmann et al., Science 244:1275, 1989), and DNA ligand (Wu et al, J. of Biol. Chem. 264:16985-16987, 1989). In one embodiment, the ribozyme is introduced into the host cell using a liposome.

Within further embodiments of the invention, additional therapeutic molecules (e.g., interferon) or facilitators may be delivered utilizing the methods described herein. Such delivery may be either simultaneous to, or before or after, the delivery of a ribozyme or vector expressing ribozymes.

PHARMACEUTICAL COMPOSITIONS As noted above, pharmaceutical compositions also are provided by this invention. These compositions contain any of the above described ribozymes, DNA molecules, vectors or host cells, along with a pharmaceutically or physiologically acceptable carrier, excipient, or diluent. Generally, such carriers should be nontoxic to recipients at the dosages and concentrations employed. Ordinarily, the preparation of such compositions entails combining the therapeutic agent with buffers, antioxidants such as ascorbic acid, low molecular weight (less than about 10 residues) polypeptides, proteins, amino acids, carbohydrates including glucose, sucrose or dextrins, chelating agents such as EDTA, glutathione and other stabilizers and excipients. Neutral buffered saline or saline mixed with nonspecific serum albumin are exemplary appropriate diluents.

Pharmaceutical compositions of the present invention may also be prepared to contain, or express (e.g., if a vector), one or more additional therapeutic molecules (e.g. , interferon) or facilitators.

In addition, the pharmaceutical compositions of the present invention may be prepared for administration by a variety of different routes, including for example intraarticularly, intradermally, intrahepatically, intramuscularly, intraperitoneally, intrathecally, intravenously (e.g., into the portal vein), or subcutaneously. In addition, pharmaceutical compositions of the present invention may be placed within containers, along with packaging material which provides instructions regarding the use of such pharmaceutical compositions. Generally, such instructions will include a tangible expression describing the reagent concentration, as well as within certain embodiments, relative amounts of excipient ingredients or diluents (e.g., water, saline or PBS) which may be necessary to reconstitute the pharmaceutical composition Pharmaceutical compositions are useful for both diagnostic or therapeutic puφoses.

THERAPEUTIC METHODS Methods of interfering with or preventing HCV viral replication, infectivity, or gene expression in a cell infected with HCV are also provided by this invention. Such methods require contacting the cell with an effective amount of ribozyme of this invention or, alternatively, by transducing the cell with an effective amount of vector having a nucleic acid molecule encoding the ribozyme. Effective amounts can be readily determined by those of skill in the art using well known methodology. When exogenously delivering the ribozyme, the RNA molecule can be embedded within a stable RNA molecule or in another form of protective environment, such as a liposome. Alternatively, the RNA can be embedded within RNase-resistant DNA counteφarts. Cellular uptake of the exogenous ribozyme can be enhanced by attaching chemical groups to the DNA ends, such as cholesteryl moieties (Letsinger et aL P.Λ .S., U.S.A., 1989).

In another aspect of the invention, the target cell is transduced under conditions favoring insertion of the vector into the target cell and stable expression of the nucleic acid encoding the HCV specific ribozyme. The target cell can include but is not limited to hepatocytes and lymphocytes. If the cell is transduced prior to HCV infection, infection of the target cell or its progeny can be prevented. Accordingly, this

aspect includes methods for interfering with or preventing HCV viral infection and/or replication in a cell by reacting the target RNA sequence with a ribozyme of this invention.

In one embodiment of this aspect of the invention, appropriate host cells such as liver cells are removed from a subject, e.g., a human patient, using methods well known in the art. The cells are then trypsinized and resuspended for ex vivo therapy. Within the cell or within the cells of an organism, a transfer vector as described above encoding one or more ribozymes is transfected into a cell or cells using methods described in Llewellyn et al., J. Mol. Biol. 795:115-123, 1987, and Hanahan, 766:557- 580, 1983. Inside the cell, the transfer vector replicates and the DNA coding for the ribozyme is transcribed by cellular polymerases to produce ribozymes which then inactivate HCV. Micromanipulation techniques such as microinjection also can be used to insert the vector into the cell so that the transfer vector or a part thereof is integrated into the genome of the cell. Transcription of the integrated material gives rise to ribozymes which then inactivate HCV. The above methods are not intended to limit the invention, but merely to exemplify various means to effect the ribozyme therapy of this invention. Other methods are detailed in Anderson, Science 256:808-813, 1992.

For ex vivo therapy, the transduced cells can be reintroduced into the patient by hepatic artery injection under conditions such that the transduced cells will integrate into the liver.

As used herein, the term "interfering with or preventing" HCV viral replication in a cell means to reduce HCV replication or production of HCV components necessary for progeny virus in a cell as compared to a cell not being transiently or stably transduced with the ribozyme or a vector encoding the ribozyme. Simple and convenient assays to determine if HCV viral replication has been reduced include an ELISA assay for the presence, absence, or reduced presence of anti-HCV antibodies in the blood of the subject (Nasoff et al., PNAS 55:5462-5466, 1991), RT- PCR (Yu et al., in Viral Hepatitis and Liver Disease 574-477, Nishioka, Suzuki and Mishiro (Eds.); Springer-Verlag Tokyo, 1994) or liver function tests. Such methods are well known to those of ordinary skill in the art. Alternatively, total RNA from transduced and infected "control" cells can be isolated and subjected to analysis by dot blot or northern blot and probed with HCV specific DNA to determine if HCV replication is reduced. Alternatively, reduction of HCV protein expression can also be used as an indicator of inhibition of HCV replication. A greater than fifty percent reduction in HCV replication as compared to control cells typically quantitates a prevention of HCV replication.

II

DIAGNOSTIC METHODS Detection and diagnosis of hepatitis C infection in humans has been somewhat problematic to date. The most commonly used methods involve detection of antibodies produced by the host against HCV structural proteins. Unfortunately, many patients remain seronegative for a variable length of time and frequently the eventual immune response can be difficult to detect. Furthermore, due to the considerable sequence variation in the HCV genome and mutations in the structural proteins, host- produced antibodies can vary significantly, requiring a large panel of antigens for accurate detection. For these reasons, a more reliable method for detection of infectious HCV would be useful, not only to test patient serum but also to screen the multitude of human-derived blood products purified for medical use.

HCV haiφin ribozymes can be applied to the detection and diagnosis of HCV infection. To accomplish this, a special reporter plasmid is generated which contains the HCV 5 '-capsid sequence upstream of the E. coli lacZ gene (nucleotides 1302-4358, Genbank accession no. J01636). This plasmid is made via a two-step cloning process. First, HCV sequences containing the 5' UTR and capsid coding region are synthesized directly from RNA that was extracted from an HCV-positive patient serum sample. The purified viral RNA is then reverse transcribed and PCR amplified with the following primers: sense (starting at 5' end of 5' UTR) 5'-GCCAGCCCCC TGATGGGG - 3' (Sequence ID No. 6) and antisense (starting at 3' end of capsid coding region) 5'-CACCTGATAA GCGGAAGC - 3' (Sequence ID No. 7). The resulting blunt-end DNA is then ligated the unique Sma I site in pCMVβ (Clontech, Palo Alto, CA). This first generation plasmid is designated pCMV-HCV-β. Second, to allow selection of this plasmid following transfection into mammalian cells, a neomycin resistance expression cassette, consisting of the SV40 early promoter driving the expression of the neomycin resistance gene, is constructed. This is accomplished by blunt-ligating a BarriiW fragment, containing the neomycin cassette obtained from pMAMneo-LUC (Clontech, Palo Alto, CA), into the unique Sail site of pCMV-HCV-β. The resulting plasmid, pCMV-HCV-β-SV-neo expresses two independent RNAs. One containing the HCV target sites upstream of the lacZ coding sequence, and the other expressing neomycin resistance for positive selection.

To generate the reporter cell line, the human hepatocellular carcinoma cell line Huh7 (Yoo et al., J. Virol. 69:32-38, 1995), is co-transfected with pCMV- HCV-β-SV-neo and an HCV haiφin ribozyme expression plasmid, pLNT-Rz. G418-

16 selected transfected Huh7 cells, containing both Rz and reporter plasmids, is then used for HCV infection diagnosis. Under normal conditions, expressed HCV Rz will cleave the HCV 5'UTR-capsid target located on the lacZ mRNA, resulting in the inhibition of β-galactosidase expression. When cells are challenged with a biological sample (e.g., patient serum samples or other blood products containing HCV, or tissue or cell samples taken from the liver), the presence of the HCV 5'UTR-capsid sequences coming from the replicating HCV will compete for the ribozyme, interfering with its ability to cleave the HCV-lacZ RNA. The result of this interference in Rz activity is an increased expression of β-galactosidase and these cells will stain blue by routine lacZ staining. Thus, any patient serum (or other biological sample) which is positive for hepatitis C virus will cause these reporter cells to turn blue.

The following examples are offered by way of illustration, and not by way of limitation.

EXAMPLES

EXAMPLE 1 CRITERIA FOR HAIRPIN RIBOZYME SITE SELECTION

Haiφin ribozymes suitable for use within the present invention preferably recognize the following sequence of RNA: NNNBNGUC(N) _K (Sequence ID Nos. 1-5) wherein the ribozyme is constructed so as to be complementary to the underlined sequences, and wherein B is C, G or U, and x is greater than or equal to 6 nucleotides (e.g., from 6 to 10 nucleotides). The sequence GUC must be conserved for all haiφin ribozymes described below. Other nucleotides ("N" as underlined above) preferably have a high degree of sequence conservation among different HCV strains in order to limit the need for multiple ribozymes against the same target site.

Briefly, sequence analysis of the complete genome of HCV-1 reveals 1 12 suitable GUC haiφin ribozyme sites in the positive strand and 125 suitable sites in the negative strand. Due to the considerable sequence variability between different HCV genotypes, however, only 34 sites in the positive strand and 36 in the negative strand represent GUC's that are 95% conserved between all known strains. The nucleotides flanking the GUC (represented as "N" above) should also preferably contain a high degree of sequence conservation in order to limit the need to design multiple ribozymes against the same target site. To that end, 28 sites have been identified in the positive strand and 30 sites in the negative strand that have no more than one base mismatch in >85% of the known strains. These sites are provided below in Table I:

Table I

Hepatitis C Ribozymes Directed Against Both (+) and (-) Strand RNA

RECOGNITION RIBOZYME: SEQUENCE": LOCATION ^b : WITHIN: SEP. LD.

HCV-1 (+) STRAND:

CR1 ACCGG ^Λ GUCCUUUCUUG 190 5' UTR 8 CR2 UAGUG GUCUGCGGAAC 146 5' UTR 9 CR3 CGGUG ^Λ GUCAGAUCGUU 424 Capsid 10 CR4 GAGCG~GUCGCAACCUC 506 Capsid 1 1

t l

CR4-7 GAGCG ^Λ GUCGCAACCU 506 Capsid 12

CR4-6 GAGCG~GUCGCAACC 506 Capsid 13

CR5 CUCCTTGUCACCCCGCG 635 Capsid 14

CR6 CUACU ^Λ GUCUUCACGCA 60 5' UTR 15

CR7 AAAGC ^Λ GUCUAGCCAUG 77 5' UTR 16

CR8 UGAGU ^Λ GUCGUGCAGCC 102 5" UTR 17

CR9 GUUGG ~ GUCGCGAAAGG 268 5' UTR 18

CR10 CGCUC " GUCGGCGCCCC 759 Capsid 19

CR1 1 AACUG ^Λ GUCGCCUACAA 1319 El 20

CR12 AUAGG ^Λ GUCAGCGGUUG 2459 NS1 21

CR13 ACCUU ^Λ GUCACCACACU 2834 NS2 22

CR14 AUGCG ^Λ GUCCCCGGUCU 3958 NS3 23

CR15 CCCCG ^Λ GUCUUCACGGA 3965 NS3 24

CR16 GACGU ^Λ GUCCGUCAUAC 4610 NS3 25

CR17 CAUGU GUCACCCAGAC 4712 NS3 26

CR18 AGACA GUCGAUUUCAG 4724 NS3 27

CR19 G CGGU GUCGCGCUCAC 4787 NS3 28

CR20 AGGUC " GUCACUAGCAC 5307 NS4 29

CR21 CUCCA ^Λ GUCCAAGCUCC 6329 NS4 30

CR22 GUUGA~ GUCGUACUCCU 7491 NS5 31

CR23 UCUUG ^Λ GUCUACCGUGA 7556 NS5 32

CR24 ACAUG ^Λ GUCUAUGCCAC 7707 NS5 33

CR25 AAGGC ^Λ GUCCACAGUUA 7823 NS5 34

CR26 ACGUG ~ GUCUCCACCCU 8133 NS5 35

CR27 GGCCITGUCGAGCUGCA 8506 NS5 36

CR28 CUACU ^Λ GUCCCAAGGGG 9134 NS5 37

HCV-1 (-) STRAND:

CNR1 GGAGU ^Λ GUCGCCCCCAA 23 "5'UTR" 38

CNR2 GGGGG ^Λ GUCCUGGAGGC 120 "5'UTR" 39

CNR3 CGGUC ^Λ GUCCUGGCAAU 183 "5'UTR" 40

CNR4 ACCCG ^Λ GUCGUCCAGGC 186 "5'UTR" 41

CNR5 UAGCA ^Λ GUCUCGCGGGG 247 "5'UTR" 42

CNR6 GCACG"GUCUACGAGAC 333 "5'UTR" 43

CNR7 CGGGG ^Λ GUCCGUGGGGC 674 "Capsid" 44

CNR8 ACGCCTGUCCUCCAGAA 821 "Capsid" 45

l

CNR9 GAGCA ^Λ GUCAUUCGUGA 959 "El" 46

CNR10 GCGGU ^Λ GUCCGCCCCCC 3308 "NS2" 47

CNR11 GUCGA ^Λ GUCAGUUGAGU 4307 "NS3" 48

CNR12 CAGCCGUCUCCGCUUG 4354 "NS3" 49

CNR13 AGAGC~GUCUGUUGCCA 4655 "NS3" 50

CNR14 UUGCA~GUCGAUCACCG 4700 "NS3" 51

CNR15 CCCGC ^Λ GUCAUAGCACU 4907 "NS3" 52

CNR16 GAGCA ^Λ GUCCUCAUUAA 6242 "NS4" 53

CNR17 UACCC~GUCACGUAGUG 6649 "NS5" 54

CNR18 ACGUITGUCGGUGGUCA 6665 "NS5" 55

CNR19 CAACCGUCCUCUUUUU 7336 "NS5" 56

CNR20 UCAGG ^Λ GUCCCCCGGCU 7532 "NS5" 57

CNR21 GACCCGUCGCUGAGAU 7547 "NS5" 58

CNR22 AGUCTTGUCAAAGGUGA 7763 "NS5" 59

CNR23 GGGGC~GUCAGCUUGCA 7873 "NS5" 60

CNR24 GUUGA ^Λ GUCAAAGCAGC 8273 "NS5" 61

CNR25 AAUUA~GUCAGGGGGCC 8398 "NS5" 62

CNR26 UAGUC GUCAGCACGCC 8455 "NS5" 63

CNR27 GUGCA ^Λ GUCCUGGAGCU 8528 "NS5" 64

CNR28 ACCUA GUCAUAGCCUC 8629 "NS5" 65

CNR29 AGUGU "GUCUAGGUCUC 8802 "NS5" 66

CNR30 GCGGG ^Λ GUCGGGCACGA 9303 "NS5" 67

"Recognition sequence is written 5' to 3'. Cleavage occurs at ^Λ . ^b Location indicates nucleotide position of cleavage site (the "G" in the "GUC") in either (+) strand, counting from 5' end, or (-) strand counting from 3' end. Numbering is using HCV-1 strain.

EXAMPLE 2 CONSTRUCTION OF HAIRPIN RIBOZYMES

Ribozyme genes (Rz) are designed by identifying highly sequence- conserved regions of the HCV genome that contain the obligatory GUC recognition signal. Rz's recognizing these sites are then designed on the basis of the nucleotide

H sequence adjacent to the GUC recognition signal. More specifically, two single- stranded DNA oligonucleotides are chemically synthesized such that, when combined and converted into double-stranded DNA, they contain the entire haiφin ribozyme, including nucleotides complementary to the target site. In addition, restriction enzyme recognition sites may be placed on either end to facilitate subsequent cloning.

For example, in order to construct ribozyme CR4, which recognizes the target sequence: GAGCGGUCGCAACCUC (Sequence I.D. No. 11 ; Table I), in either the native or tetraloop haiφin ribozyme structure (Figure 3), the following oligonucleotides are prepared:

Sense oligo (Sequence I.D. No. 68): BamHl

5'-GCGG ΓCCGGAGGTTGCAGAAGCTCACCAGAGAAACACACG-3'

Universal antisense oligo (Sequence I.D. No. 69): Mlul 5'-GGG CGCG7ACCAGGTAATATACCACAACGTGTGTTTCTCTGGT-3'

Universal tetraloop antisense oligo (Sequence I.D. No. 72): Mlul

S'-GGGΛCGCGΓACCAGGTAATATACCACGGACCGAAGTCCGTGTGTTTCTC

TGGT -3 ^*

Restriction enzyme sites (BamH I and Mlu I) are indicated in italics. Sequences complementary to the CR4 target site in HCV are underlined. The 16 bases of complementary sequence to allow annealing between the sense oligo and either the universal antisense or the universal tetraloop antisense oligonucleotide are in bold.

The appropriate oligonucleotides are annealed together and converted to double-stranded DNA using either Klenow DNA polymerase or Taq DNA polymerase. The resulting DNA is cleaved with restriction enzymes BamHl and Mlul, purified and cloned into vectors for in vitro transcription (pGEM7Z, Promega, Madison, WI; Figure

1) or for mammalian expression (pLNT; Figure 2).

Defective ribozymes (referred to as "disabled") for use as controls may be constructed as described above, with the exception that the sequence AAA. in loop 2, is changed to a UGC as shown in Figure 3.

EXAMPLE 3 CONSTRUCTION OF HCV RIBOZYME MAMMALIAN EXPRESSION VECTORS

Plasmid pMJT (Yu et al., Proc. Nat 'I Acad. Sci. USA 90:6340-6344, 1993; ATCC No. 75470), which contains the anti-U5 HIV ribozyme driven by the tRNA ^val RNA pol HI promoter, is digested with BamHl and Mlul, and the vector purified from the ribozyme fragment. The hepatitis C ribozyme genes, as described above, are excised from the pGem7Z vector (Figure 1) with BamHl and Mlul, purified, and ligated into the empty pMJT vector. The resulting vector is designated pLNT-Rz (see Figure 2), and contains the Moloney LTR driving the neomycin resistance gene and the tRN A ^vil RNA pol III promoter driving expression of the ribozyme.

EXAMPLE 4 7v VITRO CLEAVAGE ASSAYS

Ribozyme genes are cloned into in vitro transcription vectors (pGEM-7Z, Promega, Madison, Wis.) and transcribed in vitro by T7 RNA polymerase. Following transcription, reactions are treated with DNase and the ribozymes are purified by denaturing polyacrylamide gel electrophoresis. The HCV substrates are as follows: short 5'UTR substrate HCV-1 nucleotides 1-185, short capsid substrate HCV-1 nucleotides 331-698 and long substrate HCV-1 nucleotides 1-698. Substrates are transcribed in vitro in the presence [α- 32p]UTP and purified by denaturing polyacrylamide gel electrophoresis. The in vitro cleavage reactions are carried out by incubating 40 nM ribozyme with 200 nM substrate at 37°C for 0 to 60 minutes in 12 mM MgCl2/2 Mm spermidine/40 mM Tris-HCl, pH 7.5. Reactions are terminated by the addition of loading buffer (7 M urea bromophenol blue/xylene cyanol). Products of the cleavage reactions are resolved by electrophoresis on 15% acrylamide/7 M urea gels and analyzed by autoradiography.

The data in Figure 4 indicates that the CR2 Rz cleaves the short 5' UTR 185-nucleotide target RNA in a time dependent manner. Interestingly, the same Rz could not cleave a larger HCV RNA substrate that contained the entire 5' UTR (long HCV-1 substrate), presumably due to a highly folded secondary structure that is not present in the short substrate. In fact, the larger HCV substrate RNA could be cleaved by the Rz in vitro, only if the RNA substrate is heat denatured before the cleavage reaction (data not shown).

Occasionally, secondary structure in the target RNA is extensive enough to inhibit the binding and cleavage activities of haiφin ribozymes. One example is the 5 'UTR of HCV, where RNA folding inhibits the activity of CR2. Thus, "facilitator" RNA molecules can be designed to enhance the activity of ribozymes targeted to highly folded structures. Briefly, facilitator molecules are RNA's that are engineered to be complimentary to regions of the target RNA flanking the ribozyme target site. When bound to the target RNA, these molecules help to relax the secondary structure, thus enhancing the binding, and therefore the activity, of the ribozyme.

Three facilitators are designed to disrupt the folded structure in the 5 'UTR and were tested for their ability to enhance the activity of CR2. In particular, the following facilitators may be utilized: HCV269, HCV269B and HCV236 and are complimentary to bases 245-268, 242-268 and 217-235 of the 5' UTR of HCV-1 respectively. Facilitator RNAs are synthesized in vitro, added at a concentration of 40 nM to in vitro cleavage reactions containing CR2 and the long HCV- 1 substrate, and cleavage reactions are monitored over 3 hours (Figure 5). In the absence of any facilitator, less than 1% of the long HCV-1 substrate was cleaved over a period of 3 hours (Figure 5, filled squares), consistent with previous results. Addition of either facilitator molecule, HCV269 or HCV269B, enhances the substrate cleavage 3- to 3.5- fold over the control (Figure 5, filled triangles and filled diamonds). The stimulation seen with either single facilitator is similar to that seen for the reaction without facilitator, when ribozyme and substrate are heated to 90°C for 1 minute prior to incubation at 37°C (Figure 5, filled circle). When the facilitators HCV269B and HCV236 are added in combination, the reaction was stimulated 9-fold over the control reaction (Figure 5, open squares), indicating an additive to synergistic effect. These results indicate that ribozymes inhibited by secondary structure in the substrate (such as CR2) can become active against full length substrates when used with appropriately designed facilitator molecules.

Three additional Rz directed against the HCV 5' UTR (CR6, CR7, and CR8) are also tested on the 185-nucleotide short 5' UTR substrate. As indicated by the data in Figure 6, these Rz could only cleave the short 5' UTR substrate when it is heat denatured, while cleavage of the short 5 'UTR substrate by the CR2 Rz, as before, is independent of heat denaturation. Consistent with the role of secondary structure in the availability of RNA for Rz cleavage, the target sequences for CR6, CR7, and CR8 are in a location that allows extensive secondary structure, even in the short 5' UTR substrate. A potential Rz cleavage site in a highly conserved region of the HCV

Capsid protein, not containing as much secondary structure as the 5' UTR, is next

tested. Like the previously tested CR2 Rz, the Capsid-targeted CR4-8 Rz cleaves a short HCV capsid substrate RNA in vitro, without heat denaturation (Figure 7). Since the catalytic efficiency of Rz may be limited by the dissociation constant, and thus the size of the target sequence recognized, ribozymes are also generated and tested that cleave the same site as CR4-8, but recognize a target sequence that lacks one nucleotide (CR4-7) or two nucleotides (CR4-6) compared with the CR4-8 Rz. This is accomplished by changing the length of Helix 1 (Figure 3). The data in Figure 7 indicate that CR4-7 and CR4-6 are not more active than CR4-8, in fact cleavage by CR4-8 may be more efficient. As indicated by the data in Figure 8, CR-4 is capable of cleaving the long HCV substrate RNA, containing the extensive secondary structure, without heat denaturation. The autoradiogram in Figure 8 is overexposed to reveal the low molecular weight cleavage product, but lighter exposures reveal the expected higher molecular weight fragment (additional exposure not shown). As with the short substrate, cleavage by CR4-8 is identical with, or slightly more effective than, that of either CR4-7 or CR4-6.

EXAMPLE 5 CONSTRUCTION OF VECTORS, AND TESTING OF GENE EXPRESSION IN TISSUE CULTURE

As described in more detail below, reporter systems are developed to evaluate the effect of Rz expression on the expression of genes containing HCV Rz target sequences.

A. Construction of Vectors

Construction of several expression vectors is described herein (Figure 9). The HCV reporter plasmid pPur-HCV (Figure 9B) is constructed as follows: HCV sequences containing the 5'UTR and capsid coding region are synthesized directly from RNA that is extracted from an HCV-positive patient serum sample. The purified viral RNA is then reverse transcribed and PCR amplified with the following primers: sense (starting at 5' end of 5' UTR) 5'-GCCAGCCCCC TGATGGGG - 3' (Sequence ID No. 6) and antisense (starting at 3' end of capsid coding region) 5'-CACCTGATAA GCGGAAGC - 3' (Sequence ID No. 7). The resulting blunt-end DNA is then ligated into plasmid pPur (Clontech, Palo Alto, CA; Figure 9 A) that has been digested with Xbal and blunt-ended with Klenow DNA polymerase. The HCV reporter retroviral

5 vector pLNL-Pur-HCV (Figure 9D) is constructed by purifying the 2065 bp PvuII/Xbal fragment from pPur-HCV, which contains the SV40 early promoter, the puromycin resistance coding region and the HCV 5 'UTR and capsid sequences. The fragment is blunt-ended with Klenow and cloned into plasmid pLNL6 (Bender et al., J. Virol. 61 :1639-1646, 1987; Figure 9C) that has been digested with Hindlll and blunt-ended with Klenow. Both resulting HCV reporter plasmids will then produce an RNA transcript, via SV40 early promoter, that contains the HCV 5' UTR and capsid sequences on the same RNA transcript as the coding region for puromycin resistance. Each HCV ribozyme is expressed on a separate retroviral vector (pLNT-Rz) via the tRNA ^val pol III promoter. Active HCV ribozymes will cleave the Pur-HCV RNA, resulting in a cell sensitive to puromycin.

B. Co-transfection and Assay of Ribozymes

The ability of Rz to inactivate RNA containing HCV sequences is determined by co-transfection of a Rz-expressing plasmid (pLNT-Rz) with plasmids expressing an mRNA that contains HCV target sequences and codes for puromycin resistance (pPur-HCV).

Briefly, HeLa or HT1080 cells are co-transfected with pPur-HCV and various pLNT-Rz constructs using standard calcium phosphate methods. DNA molar ratios for the HCV:Rz co-transfections is 1:10, using empty pLNT vector to maintain total DNA at 20 μg. Ribozymes tested included two anti-HCV ribozymes: CR2 (against the 5' UTR) and CR4 (against capsid), and one disabled anti-HBV ribozyme, dBRl, included as a negative control. Cells are selected with 1 μg/ml puromycin starting 24 hours post transfection, and continued for up to two weeks. Puromycin-resistant colonies are visualized by crystal violet staining and counted. Ribozyme expression within the transfected cells is verified by RNase protection. Using radiolabeled antisense CR4 RNA as the RNase protection probe, the expected protected fragments are 64nt for CR4, 42nt for CR2 and 32nt for dCR4 (Figure 10; RNase Protection Assay Kit is available from Promega, Madison, WI). Expression of either Rz CR2 or Rz CR4 results in a 70-90% reduction in puromycin-resistant colonies as compared to the dBRl negative control (Figure 1 1). Results are similar in two human cell lines: HeLa (Figure 11 A) and HT1080 (Figure 1 IB). To verify that the ribozymes are exerting their effect via the HCV sequences, similar co-transfections are performed with a pPur plasmid lacking the HCV target sequence. Under these conditions, co-expression of CR2 or CR4 has no effect on the

2 H number of puromycin-resistant colonies (data not shown), indicating that the ribozymes are targeting the HCV sequence.

To determine if the activity of CR4 is dose-dependent, co-transfections are performed with varying amounts of pLNT-CR4. DNA molar ratios for the HCV:Rz co-transfections ranged from 1 :1 to 1:10, using empty pLNT vector to maintain total

DNA at 20 μg. As a negative control, disabled CR4 (dCR4) is also co-transfected at each molar ratio. Again, cells are selected with 1 μg/ml puromycin, starting 24 hours post transfection, and continued for up to two weeks. Puromycin-resistant colonies are visualized by crystal violet staining and counted. Expression of increasing amounts of CR4, but not dCR4, resulted in a dose-dependent reduction in the number of puromycin-resistant colonies in HT1080 cells (Figure 1 IC). Together, the results presented in Figure 1 1 indicate that co- expression of the HCV Rz interferes with the expression of the puromycin resistance marker, and appears to exert its effect through cleavage of the HCV target site.

C. Protection Assays

Stable expression of HCV Rz's is also tested to determine if it could protect a cell from infection with a related virus that shares some of the properties of HCV. Like HCV, retroviruses also have a positive, single-stranded, RNA genome. Thus, retroviral vector, pLNL-Pur-HCV (Figure 9D) is constructed to contain an HCV Rz target sequence in tandem with the Puromycin resistance gene. Polyclonal cell lines that stably express each of the ribozymes via the tRNA ^aI promoter are established in the human hepatocellular carcinoma cell line, HepG2, by transduction with retroviral vector, pLNT-Rz (Figure 2), followed by G418 selection. Helper-free amphotropic retroviral vector is then produced in PA317 cells, using pLNL-Pur-HCV (Figure 9D), which contains within its (+) strand RNA genome, the HCV 5' UTR and capsid sequences. The HepG2-Rz cell lines are then "challenged" by transduction with the HCV sequence-containing retroviral vector. To determine the level of transduction, cells are selected with 1 μg/ml puromycin starting 24 hours post transduction, and continued for up to two weeks. Puromycin-resistant colonies are stained and counted (see Figure 12 for a schematic representation of the experiment).

Stable expression of both the CR2 Rz or the CR4 Rz "protected" the cell from incoming retroviral vector containing HCV Rz target sequences, compared with the disabled Rz control dCR4 (Figure 13). Similar results are also shown in HeLa cells (data not shown). Together, these results confirm the previous result that the HCV ribozymes could function within human liver cells. Furthermore, these results indicate

that stable expression of HCV Rz's can inhibit "infection" with viruses that contain positive-stranded RNA genomes.

EXAMPLE 6

TESTING ADDITIONAL HCV RIBOZYME TARGET SITES IN TISSUE CULTURE

The reporter systems, as described thus far, are suitable only for the analysis of ribozymes targeted to the 5'UTR and capsid regions of HCV. In order to test the additional target sites listed in Table 1, in vivo assay systems can be designed which are capable of testing the cleavage by ribozymes of any site within the either the

(+) or (-) strand RNA.

A. Analyzing Ribozyme Cleavage in the (+) Strand

The (+) RNA strand of HCV contains the open reading frame responsible for the translation of the 3011 amino acid polyprotein. A western blot assay can be developed, using human antiserum from HCV-infected patients, to detect translation of the nucleocapsid (or capsid) protein in transfected cells (Figure 14). Briefly, transfection of HT1080 cells with pPur-HCV (see Figure 9B) results in the transcription of an mRNA containing the HCV 5 'UTR and capsid sequences. It has previously been demonstrated the 5 'UTR of HCV can act as an Internal Ribosome Entry Site (IRES), which allows protein translation to initiate from within an RNA (Wang et al., J. Virology 67:3338-3344, 1993). Thus, the transfected cells translate the capsid sequences and the 21 kD capsid protein is visualized by western blotting (Figure 14). In this experiment, only the first 1000 nucleotides of HCV are present in the mRNA, resulting in the translation of just the capsid protein. However, when more of the HCV sequence is included (anywhere up to the total 9500 basepairs), a larger polyprotein is translated and the resulting protein detected by our western blot assay is correspondingly larger (not shown). Since the capsid coding region is the first region of the HCV polyprotein to be translated, its detection can be used to visualize translation of any length polyprotein, up to the full size HCV polyprotein of nearly 350 kD. Ribozymes targeted anywhere on the (+) strand RNA would cleave the HCV mRNA, resulting in the translation of a polyprotein smaller than that translated in the absence of ribozyme. This assay can not only detect ribozyme activity, it can also be used to

quantitatively compare the activity of various ribozymes targeted to different sites anywhere on the (+) strand.

B. Analyzing Ribozyme Cleavage in the (-) Strand Since there is currently no cell culture system that supports HCV replication, and therefore the production of HCV (-) strand RNA, a luciferase reporter system can be developed to analyze the effect of ribozymes targeted against the (-) strand. Briefly, fragments of the (-) strand are cloned into the unique Hmd III site upstream of the luciferase coding region in the pGL3 vector (Figure 15; Promega, Madison, WI). These vectors, when transfected into mammalian cells, transcribe mRNA, via the SV40 early promoter, that contains the fragment of the (-) strand RNA upstream of the luciferase coding region. Ribozymes directed against the (-) strand RNA are first cloned into pLNT-Rz (see Figure 2) and then co-transfected with the ΗCV-luciferase reporter plasmid. Ribozymes capable of cleaving the (-) strand in vivo will thus result in a decrease in luciferase activity after transfection. Luciferase activity is measured by the Luciferase Assay System (Promega, Madison, WI). Similar to the assay system for testing ribozymes on the (+) strand described above, this luciferase- based assay not only can detect ribozyme activity, but can also be used to quantitatively compare the activity of various ribozymes targeted to different sites anywhere on the (-) strand.

EXAMPLE 7 CONSTRUCTION OF ADENOVIRUS AND AAV RIBOZYME DELIVERY VECTORS

A. Construction and Purification of Adenoviral Delivery Vectors

Recombinant replication-deficient (El deletion) adenoviral vectors (Av) containing ΗCV specific ribozyme genes are constructed by homologous recombination of shuttle plasmid (pAvCRz.SY; Figure 16) with Ad dl-327 (Jones and Shenk, Cell 73:181-188, 1978). Briefly, the shuttle plasmid is a pBR322-based plasmid consisting of the following elements: (1) Ad5 sequence 1-452 (Genebank accession no. M73260; containing the left inverted terminal repeat, encapsidation signals and the Ela enhancer), artificial Xbal, BamHl and Xhol sites, (2) CMV immediate/early gene promoter and enhancer (from pCMVβ expression vector, Clontech, Palo Alto, CA; Boshart et al.. Cell 47:521-530, 1985), artificial BamHl and Xhol sites, SV40 splice donor/splice acceptor sequence (from pCMVβ expression vector, Clontech, Palo Alto,

CA), artificial multiple cloning sites include BamHl, Noil, BgUl, EcoRI, Ascl, Noil, BamHl sites in a contiguous arrangement, SV40 polyadenylation (from pCMVβ expression vector, Clontech, Palo Alto, CA), artificial TfamHI, Sail and Clal sites; and (3) an Ad5 sequence used for homologous recombination (Ad5 sequences 3328-5788). HCV-specific ribozyme genes (such as CR2 and CR4 and others) or reporter genes (such as E. coli β-galactosidase gene from pCMVβ expression vector, Clontech, Palo Alto, CA) are cloned into the shuttle plasmid via the multiple cloning site (Figure 16). Recombinant adenoviral vectors are plaqued and purified from 293 cells co-transfected with both the shuttle plasmid and large C/ l-fragment of dl327. The resulting Av are plaque-purified for at least 2 more times as performed routinely (Yei et al, 1994, Human Gene Therapy 5:731-744). Av can be propagated in 293 cells and purified to high titer preparation before use in evaluating the function of anti-HCV Rz.

Since intravenous use in animals requires the preparation of large amounts of highly purified vector (e.g., Adenoviral vector preparations), within certain embodiments of the invention FPLC may be utilized to purify the relevant viral vector. For example, as shown in Figure 17, wild-type Adenovirus, used here as a model for Adenoviral vector, can be purified from clarified cell lysates by salt gradient elution of ion-exchange column chromatography. The virus, as measured by an infectivity assay, eluted as a shaφ peak at approximately 14-15 ml. It is estimated that the virus is at least 90% pure and results in yields that are comparable to other, less scalable, purification techniques, such as CsCI gradient centrifugation.

B. Construction of AAV Delivery Vectors

Recombinant AAV (rAAV) vectors containing HCV specific ribozymes can also be constructed. Potential ribozymes include, but are not limited to CR2 and CR4. Briefly, within preferred embodiments the ribozyme(s) are placed under the control of an CMV promoter, and a selectable marker, such as neomycin resistance, is placed under the control of an SV40 early promoter (Figure 18). The ribozyme can contain the bovine growth hormone (BGH) polyadenylation signal and can also contain a cleavage cassette which allows for 50% of the ribozyme molecules to be non- polyadenylated. The neo mRNA can be polyadenylated by the SV40 polyadenylation signal. The entire cassette can be flanked by AAV ITRs to allow for packaging into an AAV particle (Figure 18). To package the AAV vector, poly-1-lysine is cross-linked to the surface of adenovirus type 5 and the positively charged virus is bound to DNAs for rAAV and the AAV helper plasmid pAAV/Ad (pAAV/Ad contains all the necessary AAV complementation functions for packaging of AAV genomes; Samulski et al., J

Virology 63:3822-3828, 1989). Subsequently, the adenovirus poly-lysine complex is used to infect HeLa cells and approximately 48 hours post-infection the cells are harvested and lysed by freeze-thaw to release packaged rAAV. The cell lysate is then heated to 56°C to inactivate replication competent adenovirus and the rAAV is then purified or used crude to titer and transduce liver cells.

EXAMPLE 8 7JV VITRO CLEAVAGE OF HCV NEGATIVE GENOMIC STRAND RNA BY RIBOZYMES

Ribozyme genes which encode target HCV (-) strand RNA were synthesized and cloned into in vitro transcription vectors (pGEM-7Z, Promega, Madison, Wis.) as described in Example 2. The resultant plasmids, designated as pGem7Z-(-) ribozyme, were linearized with Nsil (immediately downstream of the cloned gene) and transcribed in vitro by T7 (Welch et al., Gene Therapy 3:994-1001, 1996). The reactions were treated with RQ1 DNase, according to information provided by the manufacturer (Promega) and the transcripts were gel purified.

HCV negative strand RNA substrates were also obtained by in vitro transcription, using the plasmid pGEM7-HCV5' for long substrate synthesis (471 bp in length). The short substrate was made by annealing 2 oligos: one with cleavage site and one with T7 promoter, and filled in with T4 DNA polymerase as described previously (Welch et al., 1996, supra)

In vitro cleavage assays were performed essentially as described above. Results are shown in Figures 19 and 20. Briefly, the blots in Figures 19 and 20 clearly indicate that the CNR3 Rz cleaves the short (19) and long (20) negative strand HCV RNA substrate.

EXAMPLE 9

CONSTRUCTION OF ADENOVIRUS AND AAV RIBOZYME DELIVERY VECTORS

WITH VARIOUS PROMOTERS

A. Construction of Adenoviral Vectors Replication-deficient adenoviral vectors expressing the catalytically active Rz (CR4) identified in Example 5 driven by various promoter were constructed

5 essentially as described in Example 7, in order to contain a tRNA ^val promoter (Yu et al., PNAS 54:1005, 1993) or CMV promoter or both. The resulting plasmids are illustrated in Figure 21 : pAvC-Rz express Rz under CMV promoter, pAvM-Rz express Rz under control of tRNA ^val promoter and pAvCM-Rz express Rz from both CMV and tRNA ^val promoters.

B. Construction of AAV Vectors

The CR4 Rz gene was also cloned into pAMFT.dBam plasmid to generate rAVV vectors expressing CR4 from tRNA ^va! promoter. Briefly, pAMFT.dBam (Figure 22) is a recombinant plasmid carrying 1) 5' and 3' inverted terminal repeats (ITR) of adeno-associated viral genome; 2) cassette for transcription of Rz gene via tRNA ^val promoter; 3) neomycin resistance marker driven by MMLV LTR. The resultant plasmid for generating AAV vectors that express CR4 ribozyme under control of tRNA ^val promoter is designated as pAAVM-CR4 (Figure 21).

EXAMPLE 10 EFFECT OF CR4 Rz IN REDUCING Hcv GENE EXPRESSION

CR4 Rz activity in reducing HCV gene expression was tested in tissue culture. Briefly, CR4 Ribozyme was expressed from pAvC-CR4 described in Example 9. Western blot to detect HCV core antigen was used to evaluate the effectiveness of ribozyme in inhibiting the intracellular synthesis of HCV capsid in tissue culture. In particular, HT1080 cells were co-transfected by pPur-HCV (see Figure 23) and pAvC-CR4 at a molar ratio of 1:10 of 1 :20 using method described in Example 5. Ribozymes tested included CR4 and disabled CR4 (dCR4). Cells were harvested at 24 hours post transfection and processed for HCV core Western blot.

As shown in Figure 24, co-transfection of pAvC-CR4 at the molar ratios tested (1 :10 and 1 :20) results in nearly complete shut off of HCV core expression while disabled form of CR4 (dCR4) had no effect (Figure 24).

S EXAMPLE 11 EFFECT OF DIFFERENT PROMOTER/VECTOR COMBINATIONS IN REDUCING HCV GENE EXPRESSION

Effect of different promoter/vector combination on CR4 Rz activity in reducing HCV gene expression was further tested in tissue culture. Briefly, the studies were done in a way similar to that described in Example 10, except that CR4 Rz was expressed from different promoter/vector constructs. Expression of CR4 from different constructs all resulted in reduced HCV core expression (Figure 25, panel A), as seen in Example 10. However, pAAVM-CR4 (adeno-associated virus DNA backbone with tRNA ^val pol III promoter expressing CR4 Rz) shows the greatest efficacy (Figure 25-A).

The activity of pAAVM-CR4 was also titered for reducing viral core expression by co-transfected the cells with HCV:Rz ratio at 1 :0, 1 :0.5, 1 :1, 1 :5, 1 :10 and 1 :20. Complete inhibition of core protein expression even at 1 :1 ratio. Significant reduction seen at 1 :0.5 ratio (Figure 25, panel B).

EXAMPLE 12 IN VIVO EXPRESSION OF RZ CR4 IN LIVER CELLS

The ability of recombinant adenovirus to deliver and express a ribozyme gene in human liver cells was evaluated essentially as follows. Briefly, adenovirus was generated that carries the CR4 gene driven by the CMV promoter (AvC-CR4). Human hepatoma cells (Huh7) were transduced with vector at m.o.i. of 0, 1 , 5, 10 or 50 and cellular RNA was harvested at 1 or 3 days post transduction.

RNase protection assay (Promega, Madison, WI) was then performed in order to measure the expression of ribozyme. Briefly, as shown in Figure 26, CR4 ribozyme was expressed equally at day 1 and day 3 post transduction and expression levels indicated a dose-response dependent on the amount of virus transduced. These results indicate that adenovirus can be used to deliver and direct expression of a ribozyme gene in human liver cells.

3/

EXAMPLE 13

INFECTIVITY OF PRIMARY NORMAL HUMAN HEPATOCYTES WITH EITHER

ADENOVIRAL OR ADENO-ASSOCIATED VIRAL VECTOR

The infectivity of primary normal human hepatocytes to the AV and

AAV vector constructs were tested essentially as described below. Briefly, primary normal human hepatocytes were infected with either AV or AAV vector that carrying beta-galactosidase gene at various MOI. At 24 hours post infection, cells were processed for lacZ staining. Number of cells stained blue due to transduction of the vectors were counted.

Results are shown in Figure 27. Briefly, both AV and AAV can efficiently infect and transduce the primary hepatocytes in culture with 100% infectivity.

From the foregoing, it will be appreciated that, although specific embodiments of the invention have been described herein for puφoses of illustration, various modifications may be made without deviating from the spirit and scope of the invention. Accordingly, the invention is not limited except as by the appended claims.

3^

SEQUENCE LISTING

(1) GENERAL INFORMATION

(l) APPLICANT Barber. Jack R Welch, Peter J Tπtz. Richard Yei , SoonPin Yu Mang

(n) TITLE OF INVENTION HEPATITIS C VIRUS RIBOZYMES

(in) NUMBER OF SEQUENCES 73

(iv) CORRESPONDENCE ADDRESS

(A) ADDRESSEE SEED and BERRY LLP

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(vi) CURRENT APPLICATION DATA

(A) APPLICATION NUMBER

(B) FILING DATE

(C) CLASSIFICATION

(vm) ATTORNEY/AGENT INFORMATION

(A) NAME McMasters, David D

(B) REGISTRATION NUMBER 33,963

(C) REFERENCE/DOCKET NUMBER 480124403PC

(ix) TELECOMMUNICATION INFORMATION

(A) TELEPHONE (206) 622-4900

(B) TELEFAX (206) 682-6031

(2) INFORMATION FOR SEQ ID NO 1

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 14 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 1

NNNBNGUCNN NNNN 14

(2) INFORMATION FOR SEQ ID NO 2

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 15 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:2:

NNNBNGUCNN NNNNN 15

(2) INFORMATION FOR SEQ ID NO:3:

(l) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 16 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:

NNNBNGUCNN NNNNNN 16

(2) INFORMATION FOR SEQ ID N0:4:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 17 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:4:

NNNBNGUCNN NNNNNNN 17

3 S-

(2) INFORMATION FOR SEQ ID NO 5

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 18 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 5

NNNBNGUCNN NNNNNNNN 18

(2) INFORMATION FOR SEQ ID NO 6

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 18 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 6

GCCAGCCCCC TGATGGGG 18

(2) INFORMATION FOR SEQ ID NO 7

(l) SEQUENCE CHARACTERISTICS (A) LENGTH 18 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 7

CACCTGATAA GCGGAAGC 18

(2) INFORMATION FOR SEQ ID NO 8

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nuclei c aci d

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 8

ACCGGGUCCU UUCUUG 16

(2) INFORMATION FOR SEQ ID NO 9

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi ) SEQUENCE DESCRIPTION SEQ ID NO 9

UAGUGGUCUG CGGAAC 16

(2) INFORMATION FOR SEQ ID NO 10

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi ) SEQUENCE DESCRIPTION SEQ ID NO 10

CGGUGGUCAG AUCGUU 16

(2) INFORMATION FOR SEQ ID NO 11

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 11

GAGCGGUCGC AACCUC 16

(2) INFORMATION FOR SEQ ID NO 12

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 15 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 12

GAGCGGUCGC AACCU 15

(2) INFORMATION FOR SEQ ID NO 13-

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 14 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 13

GAGCGGUCGC AACC 14

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(2) INFORMATION FOR SEQ ID NO 14

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 14

CUCCUGUCAC CCCGCG 16

(2) INFORMATION FOR SEQ ID NO 15

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 15

CUACUGUCUU CACGCA 16

(2) INFORMATION FOR SEQ ID NO.16.

(i) SEQUENCE CHARACTERISTICS (A) LENGTH 16 base pairs

(C) STRANDEDNESS si ngl e

(D ) TOPOLOGY l i near

(xi) SEQUENCE DESCRIPTION SEQ ID NO 16

AAAGCGUCUA GCCAUG 16

(2) INFORMATION FOR SEQ ID NO 17

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 17

UGAGUGUCGU GCAGCC 16

(2) INFORMATION FOR SEQ ID NO 18

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

HI

(xi) SEQUENCE DESCRIPTION: SEQ ID NO.18:

GUUGGGUCGC GAAAGG 16

(2) INFORMATION FOR SEQ ID NO:19:

(l) SEQUENCE CHARACTERISTICS:

(A) LENGTH. 16 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:

CGCUCGUCGG CGCCCC 16

(2) INFORMATION FOR SEQ ID NO:20:

(l) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 16 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 20

AACUGGUCGC CUACAA 16

(2) INFORMATION FOR SEQ ID NO 21

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 21

AUAGGGUCAG CGGUUG 16

(2) INFORMATION FOR SEQ ID NO 22

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 22

ACCUUGUCAC CACACU 16

3

(2) INFORMATION FOR SEQ ID NO 23

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 23

AUGCGGUCCC CGGUCU 16

(2) INFORMATION FOR SEQ ID NO 24

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 24

CCCCGGUCUU CACGGA 16

(2) INFORMATION FOR SEQ ID NO 25

(l) SEQUENCE CHARACTERISTICS (A) LENGTH 16 base pairs

i

(B) TYPE nuclei c acid

( C ) STRANDEDNESS si ngl e

( D ) TOPOLOGY l i near

(xi) SEQUENCE DESCRIPTION SEQ ID NO 25

GACGUGUCC6 UCAUAC 16

(2) INFORMATION FOR SEQ ID NO 26

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 26

CAUGUGUCAC CCAGAC 16

(2) INFORMATION FOR SEQ ID NO 27

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

Vό ^'

(xi) SEQUENCE DESCRIPTION SEQ ID NO 27

AGACAGUCGA UUUCAG 16

(2) INFORMATION FOR SEQ ID NO 28

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 28

GCGGUGUCGC GCUCAC 16

(2) INFORMATION FOR SEQ ID NO 29

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 29

AGGUCGUCAC UAGCAC 16

(2) INFORMATION FOR SEQ ID NO 30

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi ) SEQUENCE DESCRIPTION SEQ ID NO 30

CUCCAGUCCA AGCUCC 16

(2) INFORMATION FOR SEQ ID NO 31

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(x ) SEQUENCE DESCRIPTION SEQ ID NO 31

GUUGAGUCGU ACUCCU 16

H I

(2) INFORMATION FOR SEQ ID NO 32

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 32

UCUUGGUCUA CCGUGA 16

(2) INFORMATION FOR SEQ ID NO 33

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 33

ACAUGGUCUA UGCCAC 16

(2) INFORMATION FOR SEQ ID NO 34

(l) SEQUENCE CHARACTERISTICS (A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi ) SEQUENCE DESCRIPTION SEQ ID NO 34

AAGGCGUCCA CAGUUA 16

(2) INFORMATION FOR SEQ ID NO 35

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 35

ACGUGGUCUC CACCCU 16

(2) INFORMATION FOR SEQ ID NO 36

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

4?

(xi) SEQUENCE DESCRIPTION SEQ ID N0:36

GGCCUGUCGA GCUGCA 16

(2) INFORMATION FOR SEQ ID NO.37-

(l) SEQUENCE CHARACTERISTICS:

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION. SEQ ID NO.37

CUACUGUCCC AAGGGG 16

(2) INFORMATION FOR SEQ ID N0:38-

(l) SEQUENCE CHARACTERISTICS:

(A) LENGTH- 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY linear

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(xi) SEQUENCE DESCRIPTION SEQ ID NO-38

GGAGUGUCGC CCCCAA 16

(2) INFORMATION FOR SEQ ID NO-39

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi ) SEQUENCE DESCRIPTION- SEQ ID NO 39

GGGGGGUCCU GGAGGC 16

(2) INFORMATION FOR SEQ ID NO'40

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH. 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS. single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION. SEQ ID NO 40

CGGUCGUCCU GGCAAU 16

S/

(2) INFORMATION FOR SEQ ID NO 41

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 41

ACCCGGUCGU CCAGGC 16

(2) INFORMATION FOR SEQ ID NO 42

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO-42

UAGCAGUCUC GCGGGG 16

(2) INFORMATION FOR SEQ ID NO 43

(i) SEQUENCE CHARACTERISTICS (A) LENGTH 16 base pairs

5 ^

(B) TYPE nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:43:

GCACGGUCUA CGAGAC 16

(2) INFORMATION FOR SEQ ID NO-44:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 16 base pairs

(B) TYPE, nucleic acid

(C) STRANDEDNESS. single

(D) TOPOLOGY, linear

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:44:

CGGGGGUCCG UGGGGC 16

(2) INFORMATION FOR SEQ ID N0:45:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH 16 base pairs

(B) TYPE- nucleic acid

(C) STRANDEDNESS. single

(D) TOPOLOGY, linear

6-

(xi) SEQUENCE DESCRIPTION SEQ ID NO 45

ACGCCGUCCU CCAGAA 16

(2) INFORMATION FOR SEQ ID NO 46

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 46

GAGCAGUCAU UCGUGA 16

(2) INFORMATION FOR SEQ ID NO 47

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 47

GCGGUGUCCG CCCCCC 16

(2) INFORMATION FOR SEQ ID NO 48

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 48

GUCGAGUCAG UUGAGU 16

(2) INFORMATION FOR SEQ ID NO 49

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 49

CAGCCGUCUC CGCUUG 16

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(2) INFORMATION FOR SEQ ID NO 50

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 50

AGAGCGUCUG UUGCCA 16

(2) INFORMATION FOR SEQ ID NO 51

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 51

UUGCAGUCGA UCACCG 16

(2) INFORMATION FOR SEQ ID NO 52

(l) SEQUENCE CHARACTERISTICS (A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 52

CCCGCGUCAU AGCACU 16

(2) INFORMATION FOR SEQ ID NO 53

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 53

GAGCAGUCCU CAUUAA 16

(2) INFORMATION FOR SEQ ID NO 54

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 54

UACCCGUCAC GUAGUG 16

(2) INFORMATION FOR SEQ ID NO 55

(ι) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 55

ACGUUGUCGG UGGUCA 16

(2) INFORMATION FOR SEQ ID NO 56

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 56

CAACCGUCCU CUUUUU 16

(2) INFORMATION FOR SEQ ID NO 57

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 57

UCAGGGUCCC CCGGCU 16

(2) INFORMATION FOR SEQ ID NO 58

(ι) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 58

GACCCGUCGC UGAGAU 16

£<l

(2) INFORMATION FOR SEQ ID NO 59

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY l near

(xi) SEQUENCE DESCRIPTION SEQ ID NO 59

AGUCUGUCAA AGGUGA 16

(2) INFORMATION FOR SEQ ID NO 60

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 60

GGGGCGUCAG CUUGCA 16

(2) INFORMATION FOR SEQ ID NO 61

(i) SEQUENCE CHARACTERISTICS (A) LENGTH 16 base pairs

(B) TYPE nucleic acid

( C) STRANDEDNESS si ngl e

( D) TOPOLOGY l i near

(xi) SEQUENCE DESCRIPTION SEQ ID NO 61

GUUGAGUCAA AGCAGC 16

(2) INFORMATION FOR SEQ ID NO 62

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 62

AAUUAGUCAG GGGGCC 16

(2) INFORMATION FOR SEQ ID NO 63

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

G t

(xi) SEQUENCE DESCRIPTION SEQ ID NO 63:

UAGUCGUCAG CACGCC 16

(2) INFORMATION FOR SEQ ID NO:64.

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH. 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS. single

(D) TOPOLOGY linear

(xi ) SEQUENCE DESCRIPTION: SEQ ID NO.64-

GUGCAGUCCU GGAGCU 16

(2) INFORMATION FOR SEQ ID N0:65-

(l) SEQUENCE CHARACTERISTICS-

(A) LENGTH: 16 base pairs

(B) TYPE- nucleic acid

(C) STRANDEDNESS- single

(D) TOPOLOGY linear

6Λ (xi) SEQUENCE DESCRIPTION SEQ ID NO 65

ACCUAGUCAU AGCCUC 16

(2) INFORMATION FOR SEQ ID NO 66

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 66

AGUGUGUCUA GGUCUC 16

(2) INFORMATION FOR SEQ ID NO 67

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 16 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 67

GCGGGGUCGG GCACGA 16

& 3

(2) INFORMATION FOR SEQ ID NO.68

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 41 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 68

GCGGATCCGG AGGπGCAGA AGCTCACCAG AGAAACACAC G 41

(2) INFORMATION FOR SEQ ID NO 69

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 45 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 69

GGGACGCGTA CCAGGTAATA TACCACAACG TGTGGTTTCT CTGGT 45

(2) INFORMATION FOR SEQ ID NO 70

(l) SEQUENCE CHARACTERISTICS (A) LENGTH 53 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 70

GGAGGUUGCA GAAGCUCACC AGAGAAACAC ACGUUGUGGU AUAUUACCUG GUA 53

(2) INFORMATION FOR SEQ ID NO 71

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 17 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO 71

GAGCGGUCGC AACCUCC 17

(2) INFORMATION FOR SEQ ID NO 72

(l) SEQUENCE CHARACTERISTICS

(A) LENGTH 53 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS single

(D) TOPOLOGY linear

>£

(x ) SEQUENCE DESCRIPTION SEQ ID NO 72

GGGACGCGTA CCAGGTAATA TACCACGGAC CGAAGTCCGT GTGTTTCTCT GGT 53

(2) INFORMATION FOR SEQ ID NO 73

(i) SEQUENCE CHARACTERISTICS

(A) LENGTH 62 base pairs

(B) TYPE nucleic acid

(C) STRANDEDNESS- single

(D) TOPOLOGY linear

(xi) SEQUENCE DESCRIPTION SEQ ID NO-73

GGAGGUUGCA GAAGCUCACC AGAGAAACAC ACGGACUUCG GUCCGUGGUA UAUUACCUGG 60

UA 62