TITLE OF THE INVENTION

Herbal composition and medicament for promoting poultry health.

This application claims priority from Indian Patent Application No. 1050/MUM/2011 filed on 31 ^st March 2011.

FIELD OF THE INVENTION:

The present invention relates to herbal composition and poultry supplement thereof for promoting poultry health.

The present invention relates to herbal composition and medicament thereof for treatment of Coccidiosis.

BACKGROUND OF THE INVENTION:

Poultry comes from the French/Norman word, poule, itself derived from the Latin word Pullus, which means small animal. Poultry is a category of domesticated birds kept by humans for the purpose of collecting their eggs, or killing for their meat and/or feathers. The poultry includes chickens, ducks, guinea fowl, turkeys, geese as well as live birds, wild birds (including caged birds).

Poultry provides globally important sources of animal protein and are amongst the most intensively reared of all livestock species. Diseases of poultry are therefore of major concern, both locally and on an international scale. It is also important to consider the concept of, and the need for, bio-security or the range of management procedures designed to protect livestock from infection. In contrast to modern poultry production, village-based poultry production is often characterized by a range of diseases occurring at the same time. Most often free-range poultry have sub-clinical infections with a high number of endoparasites and ectoparasites. The scheme illustrates about the problems which are associated with poultry and diseases caused to poultry which can be further spread and measures which can be taken to promote healthy poultry.

Scheme 1

The low productivity of poultry in traditional systems is mainly due to high mortality, which is caused by mismanagement, diseases, lack of nutritional feeding and predators. In most family poultry flocks, disease is an important problem. Diagnosis, treatment and / or prevention of diseases are of major importance to any attempts at increasing productivity. In commercial production systems chickens are therefore routinely vaccinated against major diseases such as Newcastle Disease, Mareks Disease, Infectious bronchitis, Avian Influenza and others, depending on the specific situations and recommendations in each country.

Coccidiosis is also referred as Coccidia, Coxy, or Cocci is a major disease of poultry caused by a protozoan-type parasite. This parasite lives and multiplies in the intestinal tract and causes tissue damage. This damage can interfere with the food digestion and nutrient absorption, as well as causing dehydration and blood loss. The tissue damage can also expose the bird to bacterial infections, like Clostridium and Salmonella. Diseases that suppress the bird's immune system may act with coccidiosis to produce a more severe problem. For example, Marek's Disease may interfere with the development of coccidiosis immunity and Infectious Bursal Disease may exacerbate a coccidia infection. Clinical signs of coccidiosis can range from none to bloody droppings, watery diarrhea (flushing), weight loss, paleness, sick bird appearance (ruffled feathers, huddling, depression). Affected birds do not eat and will sometimes march from feed pan to feed pan, vocalizing a high pitched cry. Mortality can range from mild to severe, depending on the species of coccidia involved. All ages of poultry are susceptible to infection, but the disease usually resolves itself around 6 - 8 weeks of age. The birds are most sensitive between 3 - 5 weeks of age, when the coccidia oocysts are the most numerous in feces or litter. Many times the disease is subclinical and the flock may only show poor weight gains or feed conversions at the end of grow-out.

Birds infected with coccidia may shed oocysts in their feces for days or weeks. Susceptible birds in the same flock ingest the infective or sporulated oocysts in the litter, soil, feed or water and become infected. Infected feces or litter on boots, clothing, equipment, darkling beetles or in dust can then spread the disease to other houses or farms. Outbreaks occur when susceptible birds ingest massive numbers of sporulated oocysts. The best conditions for oocyst sporulation occur in wet litter with warm temperatures. Oocysts can survive up to four years in the environment if conditions are right.

Coccidiosis causes the poultry industry to suffer a considerable economic loss, especially in the production of broiler chickens. Chickens are susceptible to at least 1 1 species of coccidia.

Coccidiosis is prevented through good sanitation and litter management. Coccidia oocysts are very resistant to harsh environmental conditions and common disinfectants, so changing the litter after each brood will reduce the number of oocysts the birds can ingest.

Anticoccidial medication is commonly added to poultry feed as a preventative measure against the disease. These medications act to either kill or stop development of the parasite. Some medications, like ionophors - Coban, Avatec, allow leakage of oocysts, so the birds can build up immunity to the parasite. Changing types of anticoccidial medications reduces the chance of the coccidia parasite to become resistant to one type of medication. The introduction of the anticoccidal medication in the bird's feed does not guarantee the bird's consumption of the same. Empty feed pans, hot weather or another disease problem that will cause the birds to stop eating the medicated feed, does not stop them from picking at the litter and ingesting more coccidia oocysts. Treatment of coccidiosis is important tool to control an outbreak. Amprolium, sulfadimethoxine, and sulfaquinoxaline are effective against coccidia as a treatment, however toxicity and withdrawal times need to be a concern when using sulfa-drugs.

Herbs like erberis aristata, Holarrhena antidyssentrica, Momordica dioica, Melia azedarah, Melia azadirachta are known in the art for the treatment of coccidiosis.

According to our knowledge, nobody has researched on the combination of Allium cepa, Allium satuvum and Peucedanum grande for the promoting poultry health as well as for treatment of Coccidiosis.

OBJECT OF THE INVENTION:

An object of the invention is to provide herbal composition and poultry supplement thereof for promoting poultry health.

Another object of the invention is to provide herbal composition and poultry supplement thereof for promoting poultry health; said composition and supplementary thereof showing a good impact on gastro intestinal tract.

Yet another object of the invention is to provide herbal composition and poultry supplement thereof for promoting poultry health; which is efficient and showing increased efficacy.

Still another object of the invention is to provide herbal composition and poultry supplement thereof for promoting poultry, health; which is made from readily available sources thereby making it economic.

An additional object of the invention is to provide herbal composition and medicament thereof for treatment of Coccidiosis.

Another additional object of the invention is to provide herbal composition and medicament thereof for treatment of Coccidiosis; which is efficient and showing increased efficacy.

Yet another additional object of the invention is to provide herbal composition and medicament thereof for treatment of Coccidiosis; which is made from readily available sources thereby making it economic. DETAILED DESCRIPTION OF THE INVENTION:

The present invention as described below, it is to be understood that this invention is not limited to particular methodologies and materials described, as these may vary as per the person skilled in the art. I ⁺ . is also to be understood that the terminology used in the description is for the purpose of describing the particular embodiments only, and is not intended to limit the scope of the present invention.

Before the present invention is described, it is to be understood that unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Further, it is to be understood that the present invention is not limited to the methodologies and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are described, as these may vary within the specification indicated. Unless stated to the contrary, any use of the words such as "including," "containing," "comprising," "having" and the like, means "including without limitation" and shall not be construed to limit any general statement that it follows to the specific or similar items or matters immediately following it. Embodiments of the invention are not mutually exclusive, but may be implemented in various combinations. The described embodiments of the invention and the disclosed examples are given for the purpose of illustration rather than limitation of the invention as set forth the appended claims. Further the terms disclosed embodiments are merely exemplary methods of the invention, which may be embodied in various forms.

It is also to be understood that the terms "a", "an", "the" and like are words for the sake of convenience and are not to be construed as limiting terms. Moreover, it will be understood that the illustrations are for the purpose of describing a particular exemplary embodiment of the invention and are not limited to the invention thereto.

It is also understood that the terms "sieving", "washing", grinding", "drying", "extraction" used herein intend to cover all kinds of conventional methods used for the said purpose carried out at room temperature unless specified particularly herein.

The term "herbal composition", as used herein, intends to cover herbal medicament useful in curing bloat. The herbal medicament of the present invention is simple, natural, attractive and convenient to use. The manufacture method for herbal medicament is simple and the effect is excellent; the price is low. The herbal medicament of the invention is useful in the treatment of bone fracture, dislocation or osteoporosis or similar conditions. Further, the herbal medicament of the invention also shows antimicrobial activity.

To achieve our object of the invention, we studied the combination of Allium cepa, Allium sativum and Peucedanum grande in different proportions and their effect on promoting poultry health as well as for the treatment of Coccidiosis.

Peucedanum grande is commonly known as 'Hill carrot' (also known as 'Bafli' in Marathi) which is an herb from the carrot family. Bafli is also called Wild Carrot and is found in the Western Ghats. The fruit is used in curries as a flavouring agent. The infusion of the fruit is used in doses of ½ to 1 ounce like that of fennel seeds, as carminative, diuretic and stimulant in flatulency, gastric and intestinal disorders, etc.

The botanical name of ga lic is Allium sativum. Garlic is a good source of vitamins A, B l , B2, and C, enzymes, minerals and trace elements. Garlic has so many benefits for humans so it is not surprising that it can also benefit many animals and birds including poultry. It can help in maintaining a clear respiratory system and reduce infections or worms in the gut. A natural granular supplement containing garlic supports a healthy immune system in all poultry and waterfowl, and will help to keep clear airways and prevent respiratory problems.

The botanical name of onion is Allium cepa. Onions are a major source of polyphenols in general, and also of flavonoids (a very important subdivision of polyphenols). They can also vary greatly in their polyphenol and flavonoid content Polyphenols are one of the largest categories of phytonutrients in food. This category includes all flavonoids as well as tannins. The sulfur compounds in onion can lower blood levels of cholesterol and triglycerides, and also improve cell membrane function in red blood cells.

While onion is not as well researched as garlic in terms of specific inflammatory health problems like rheumatoid arthritis or allergic airway inflammation, this Allium vegetable has nevertheless been shown to provide important anti-inflammatory benefits. In animal studies, onions have shown potential for improvement of blood sugar balance. Most of the animal studies have been conducted on rats, and most have used onion juice or onion extract as the form of onion tested. While not as well researched as garlic in terms of antibacterial benefits, onion has nevertheless been shown to help prevent bacterial infection. Onions are a very good source of immune-supportive vitamin C, blood-sugar-balancing chromium, and heart-healthy dietary fiber. They are also a good source of enzyme-activating manganese and molybdenum; heart-healthy vitamin B6, foliate, and potassium; energy-producing phosphorus; and bone- supportive copper.

Therefore, research has been always carried out with new medicaments which can be used to promote poultry health.

According to one of the embodiments of the invention, there is provided herbal composition for promoting poultry health;

said composition comprising predefined amount of Peucedanum grande; predefined amount of Allium cepa and predefined amount of Allium sativum.

Preferably, the said herbal composition comprising 80% to 90 % of Peucedanum grande, 10% to 15 % of Allium cepa, 5% to 10 % of Allium satuvum. All percentages are percent by weight of the mixture.

In another embodiment of the invention, the said herbal composition of the invention is used for treatment of Coccidiosis. The said herbal composition showed a good impact on gastro intestinal tract.

Thus, the said herbal composition is used for promoting poultry health as well as for the treatment of Coccidiosis. The said herbal composition showed food impact on gastro intestinal tract. The composition is found to be efficient and showed enhanced efficacy in promoting poultry health as well as in the. treatment of Coccidiosis.

Particularly, se'eds of Peucedanum grande is used in the present invention.

More particularly, 80% to 90 % of the seeds of Peucedanum grande is used in the present invention. Particularly, bulbs of Allium cepa is used in the present invention.

More particularly, 10% to 15 % of bulbs of Allium cepa is used in the present invention.

Particularly, clove of Allium satuvum is used in the present invention.

Particularly, 5% to 10 % of the clove of Allium satuvum is used in the present invention.

More preferably, the said herbal composition comprising 80% to 90 % of seeds of Peucedanum grande, 10% to 15 % of bulb of Allium cepa, 5% to 10 % of clove of Allium satuvum.

Presently, we have used seeds of Peucedanum grande, bulb of Allium cepa and clove of Allium satuvum. However, the use of other parts of Peucedanum grande, Allium cepa and Allium satuvum in the composition should be considered within the scope of the invention.

Instead of paste or powder or juice of the seeds of Peucedanum grande, bulb of Allium cepa and clove of Allium satuvum, the composition may comprises the extract like a whole plant extract, a rhizome extract, a leaf extract, a seed extract, a root extract, a bark extract, a flower extract, a fruit extract, and a combination thereof.

Instead of paste or powder of the seeds of Peucedanum grande and paste or juice of bulb of Allium cepa and clove of Allium satuvum; optionally the said composition comprises an extract of the seeds of Peucedanum grande and the bulb of Allium cepa and the clove of Allium satuvum.

The extract is generally prepared in a solvent, typically water, alcohol like methanol or ethanol or combination thereof. However, other solvents and solutions compatible with plant usage and habitat may also be used. Typically, 50 ml of the solvent like water, alcohol like methanol or ethanol or a mixture thereof is used for the extraction. A mixture of water and alcohol used in the extraction is in the proportion of 60:40.

The composition is found to be efficient and has showed enhanced efficacy in both humans and animals. The animals by definition are a major group of multicellular, eukaryotic organisms of the kingdom Animalia or Metazoa. Thus, "animal" intends to cover all the mammals including human beings, domestic pets as well as wild animals, birds, etc.

According to yet another embodiment of the invention there is provided a method for the preparation of said herbal composition of the invention;

said method comprises;

a. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande followed by drying and making paste or powder by conventional means;

b. sieving followed by washing and sieving again predefined amount of bulbs of Allium cepa followed by drying and making paste or juice by conventional means;

c. sieving followed by washing and sieving again predefined amount of cloves of Allium satuvum followed by drying and making paste or juice by conventional means; and d. mixing paste or powder obtained in step (a) to pastes or juices obtained in steps (b) and (c) together followed by sieving to obtain synergistic composition.

The method optionally comprises :

e. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande, predefined amount of bulbs of Allium cepa and predefined amount of cloves of Allium satuvum by drying by conventional means; and

f. making paste or juice of all ingredients obtained in step (e) by conventional means followed by sieving to obtain synergistic composition.

The said herbal composition is used for treatment of Coccidiosis as well as for promoting poultry health. The said herbal composition showed a good impact on gastro intestinal tract.

Instead of paste or powder or juice of the seeds of Peucedanum grande, bulb of Allium cepa and clove of Allium satuvum, the composition may comprises the extract like a whole plant extract, a rhizome extract, a leaf extract, a seed extract, a root extract, a bark extract, a flower extract, a fruit extract, and a combination thereof.

Instead of paste or powder of the seeds of Peucedanum grande and paste or juice of bulb of Allium cepa and clove of Allium satuvum; optionally the said medicament comprises an extract of the seeds of Peucedanum grande and the bulb of Allium cepa and the clove of Allium satuvum. The extract is generally prepared in a solvent, typically water, alcohol like methanol or ethanol or combination thereof. However, other solvents and solutions compatible with plant usage and habitat may also be used. Typically, 50 ml of the solvent like water, alcohol like methanol or ethanol or a mixture thereof is used for the extraction. A mixture of water and alcohol used in the extraction is in the proportion of 60:40.

According to yet another embodiment of the invention there is provided a method for the preparation of said herbal composition of the invention;

said method comprises;

g. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande followed by drying and making paste or powder by conventional means;

h. sieving followed by washing and sieving again predefined amount of bulbs of Allium cepa followed by drying and making paste or juice by conventional means;

i. sieving followed by washing and sieving again predefined amount of cloves of Allium satuvum followed by drying and making paste or juice by conventional means;

j. mixing paste or powder obtained in step (g) to pastes or juices obtained in steps (h) and (i) together followed by sieving to obtain synergistic composition; and

k. extracting the paste or juice obtained in steps (j) with solvent at controlled temperature between 50°C to 70°C and converting the same into semisolid or solid form of synergistic composition; or

1. subjecting the paste or juice obtained in steps (j) to steam distillation and converting the distillate into semisolid or solid form of synergistic composition.

Optionally, the extraction or steam distillation is carried out separately at each step (g), (h) and (i) and mixing extracts or distillate to prepare the synergistic composition of the invention.

The method optionally comprises :

m. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande, predefined amount of bulbs of Allium cepa and predefined amount of cloves of Allium satuvum followed by drying by conventional means; and

n. making paste or juice of all ingredients of step (m) by conventional means followed by sieving to obtain synergistic composition; and o. extracting the paste or juice obtained in steps (n) with solvent at controlled temperature between 50°C to 70°C and converting the same into semisolid or solid form as the synergistic composition; or

p. subjecting the paste or juice obtained in steps (n) to distillation and converting the distillate into semisolid or solid form as the synergistic composition.

The extraction is generally carried out in a solvent, typically water, alcohol like methanol or ethanol or combination thereof. However, other solvents and solutions compatible with plant usage and habitat may also be used. A mixture of water and alcohol used for extraction is in the proportion of 60:40. The 50 ml of either water or alcohol or the mixture of water and alcohol is used for the extraction.

The extraction can be carried out in a temperature controlled extraction vessel at a temperature between 50°C to 70°C. Preferably, the extraction can be carried out at controlled temperature between 55°C to 65°C.

To prepare the composition of the present invention, the components are first thoroughly washed and dried well. Thereafter they are sieved to remove remaining impurities and preferably, washed again with sterile water.

The composition of the present invention based on the above herbs acts as a synergistic composition having unexpected and new properties in promoting poultry health. The said composition also shows increased efficacy in the treatment of Coccidiosis and its impact on gastro intestinal tract.

The combination of above-mentioned components in promoting poultry health and in the treatment of Coccidiosis and its impact on gastro intestinal tract is therefore, truly inventive and non obvious.

According to yet another embodiment of the invention, there is provided herbal medicament for treatment of Coccidiosis;

said medicament comprising predefined amount of composition of the invention along with either suitable pharmaceutically acceptable excipient, vehicle and / or adjuvant suitable for administering orally or topically or injectable including intravenous, subcutaneous, etc or intramuscular, by any other suitable route of administration. According to yet another embodiment of the invention, there is provided herbal poultry supplements for promoting poultry health;

said supplements comprising predefined amount of composition of the invention along with suitable poultry supplementary base.

The herbal poultry supplements showed a good impact on gastro intestinal tract.

According to still yet another embodiment of the invention there is provided method for the preparation of said herbal medicaments for treatment of Coccidiosis;

said method comprises;

i. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande followed by drying and making paste or powder by conventional means;

ii. sieving followed by washing and sieving again predefined amount of bulbs of Allium cepa followed by drying and making paste or juice by conventional means;

iii. sieving followed by washing and sieving again predefined amount of cloves of Allium satuvum followed by drying and making paste or juice by conventional means;

iv. mixing paste or powder obtained in step (i) to pastes or juices obtained in steps (ii) and (iii) together followed by sieving to obtain synergistic composition; and

v. optionally adding either suitable excipient, vehicle or adjuvant to the composition obtained in step (iv);

vi. converting the composition obtained in step (iv) or (v) into medicament by conventional method;

said medicament for administering orally or topically or injectable including intravenous, subcutaneous, etc or by any other suitable route of administration.

The method optionally comprises :

vii. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande, predefined amount of bulbs of Allium cepa and predefined amount of cloves of Allium satuvum followed by drying by conventional means; and viii. making paste or juice of all ingredients of step (vii) by conventional means followed by sieving to obtain synergistic composition; ix. optionally adding either suitable excipient, vehicle or adjuvant to the composition obtained in step (viii);

x. converting the composition obtained in step (viii) or (ix) into medicament by conventional method;

said medicament for administering orally or topically or injectable including intravenous, subcutaneous, etc or by any other suitable route of administration.

The method optionally comprises :

xi. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande followed by drying and making paste or powder by conventional means;

xii. sieving followed by washing and sieving again predefined amount of bulbs of Allium cepa followed by drying and making paste or juice by conventional means;

xiii. sieving followed by washing and sieving again predefined amount of cloves of Allium satuvum followed by drying and making paste or juice by conventional means;

xiv. mixing paste or powder obtained in step (xi) to pastes or juices obtained in steps (xii) and (xiii) together followed by sieving to obtain synergistic composition; and xv. extracting the paste or juice obtained in steps (xiv) with solvent at controlled temperature between 50°C to 70°C and converting the same into semisolid or solid form of synergistic composition; or

xvi. subjecting the paste or juice obtained in steps (xiv) and converting the distillate into semisolid or solid form of synergistic composition;

xvii. optionally adding either suitable excipient, vehicle or adjuvant to the composition obtained in step (xiv) or (xv) or (xvi);

xviii. converting the composition obtained in step (xv) or (xvi) or (xvii) into medicament by conventional method;

said medicament for administering orally or topically or injectable including intravenous, subcutaneous, etc or by any other suitable route of administration.

Optionally, the extraction or steam distillation can be carried out separately at each step (xi), (xii) and (xiii) and mixing extracts or distillate to prepare the synergistic composition of the invention. The method optionally comprises :

xix. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande, predefined amount of bulbs of Allium cepa and predefined amount of cloves of Allium satuvum followed by drying by conventional means;

xx. making paste or juice of all ingredients of step (xix) by conventional means followed by sieving to obtain synergistic composition;

xxi. extracting the paste or juice obtained in steps (xx) with solvent at controlled temperature between 50°C to 70°C and converting the same into semisolid or solid form of synergistic composition; or

xxii. subjecting the paste or juice obtained in step (xx) to distillation and converting the distillate into semi solid or solid form of synergistic composition;

xxiii. optionally adding either suitable excipient, vehicle or adjuvant to the composition obtained in step or (xxi) or (xxii); and

xxiv. converting the composition obtained in step (xxi) or (xxii) or (xxiii) into medicament by conventional method;

said medicament for administering orally or topically or injectable including intravenous, subcutaneous, etc or by any other suitable route of administration.

The extraction is generally carried out in a solvent, typically water, alcohol like methanol or ethanol or combination thereof. However, other solvents and solutions compatible with plant usage and habitat may also be used. A mixture of water and alcohol used for extraction is in the proportion of 60:40. The 50 ml of either water or alcohol or the mixture of water and alcohol is used for the extraction.

The extraction can be carried out in a temperature controlled extraction vessel at a temperature between 50°C to 70°C. Preferably, the extraction can be carried out at controlled temperature between 55°C to 65°C.

According to yet another embodiment of the invention there is provided a method for the preparation of said herbal poultry supplement for promoting poultry health;

said method comprises; A. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum gra de followed by drying and making paste or powder by conventional means;

B. sieving followed by washing and sieving again predefined amount of bulbs of Allium cepa followed by drying and making paste or juice by conventional means;

C. sieving followed by washing and sieving again predefined amount of cloves of Allium satuvum followed by drying and making paste or juice by conventional means;

D. mixing paste or powder obtained in step (A) to pastes or juices obtained in steps (B) and (C) together followed by sieving to obtain synergistic composition; and

E. adding suitable supplementary base to the composition obtained in step (D) by conventional method to obtain poultry supplement;

said supplement can be administering orally or by any other suitable route of administration.

The method optionally comprises :

F. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande, predefined amount of bulbs of Allium cepa and predefined amount of cloves of Allium satuvum followed by drying by conventional means; and

G. making paste or juice of all ingredients of step (F) by conventional means followed by sieving to obtain synergistic composition;

H. adding suitable supplementary base to the composition obtained in step (G) by conventional method to obtain poultry supplement;

said supplement can be administering orally or by any other suitable route of administration.

The method optionally comprises:

I. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande followed by drying and making paste or powder by conventional means;

J. sieving followed by washing and sieving again predefined amount of bulbs of Allium cepa followed by drying and making paste or juice by conventional means;

K. sieving followed by washing and sieving again predefined amount of cloves of Allium satuvum followed by drying and making paste or juice by conventional means;

L. mixing paste or powder obtained in step (I) to pastes or juices obtained in steps (J) and (K) together followed by sieving to obtain synergistic composition; and M. extracting the paste or juice obtained in steps (L) with solvent at controlled temperature between 50°C to 70°C and converting the same into semisolid or solid form of synergistic composition; or

N. subjecting the paste or juice obtained in steps (L) to distillation and converting the distillate into semi solid or solid form of synergistic composition;

O. adding suitable supplementary base to the composition obtained in step (M) or (N) by conventional method to obtain poultry supplement;

said supplement can be administering orally or by any other suitable route of administration.

The extraction or steam distillation can be carried out separately at each step (I), (J) and (K) and mixing extracts or distillate to prepare the synergistic composition of the invention.

The method optionally comprises :

P. sieving followed by washing and sieving again predefined amount of seeds of Peucedanum grande, predefined amount of bulbs of Allium cepa and predefined amount of cloves of Allium satuvum followed by drying by conventional means;

Q. making paste or juice of all the ingredients of step (P) by conventional means followed by sieving to obtain synergistic composition;

R. extracting the paste or juice obtained in steps (Q) with solvent at controlled temperature between 50°C to 70°C and converting the same into semisolid or solid form of synergistic composition; or

S. subjecting the paste or juice obtained in steps (Q) to distillation and converting the distillate into powder form as the synergistic composition;

T. adding suitable supplementary base to the composition obtained in step (R) or (S) by conventional method to obtain poultry supplement;

said supplement can be administering orally or by any other suitable route of administration. In still another embodiment of the invention, the said medicament can be administered orally or topically or injected intravenously, subcutaneously, etc or by any other suitable route of administration.

In oral administration, the herbal medicament may be in the form including but not limited to tinctures, herbal wine, elixirs, tisanes, decoctions, powder, juice, syrup, tablets, granules, capsules, etc. In topical administration, the herbal medicament may be in the form including but not limited to oils, salves, balms, cream, lotion, spray, poultices, compresses, etc.

In administration by injection, the herbal medicament may be in the form including but not limited to bolus, intravenous injection, subcutaneous injection, intrammuscular injection, intrathecal, etc.

Particularly, Tinctures are alcoholic extracts of herbs; usually obtained by combining 100% pure ethanol (or a mixture of 100% ethanol with water) with the herb. A completed tincture has ethanol percentage of at least 25% (sometimes up to 90%). The term tincture is sometimes applied to preparations using other solvents than ethanol.

Herbal wine and elixirs are alcoholic extract of herbs; usually with an ethanol percentage of 12-38%. Herbal wine is a maceration of herbs in wine, while an elixir is a maceration of herbs in spirits (e.g., vodka, grappa, etc.).

Tisanes are hot water extracts of herb.

Decoctions are long-term boiled extract of herb.

Powder and Juices: In this whole herb is consumed either in dried form (herbal powder), or fresh juice, (fresh leaves and other plant parts).

Syrups - Extracts of herbs made with syrup or honey. Sixty five parts of sugar are mixed with 35 parts of water and herb. The whole is then boiled and macerated for three weeks.

Salves, oils, balms, creams and lotions: Most topical applications are oil extractions of herbs. Taking a food grade oil and soaking herbs in it for anywhere from weeks to months allows certain photochemicals to be extracted into the oil. This oil can then be made into salves, creams, lotions, or simply used as oil for topical application.

Poultices and compresses: The whole herb (or the appropriate part of the plant) usually crushed or dried and re-hydrated with a small amount of water and then applied directly in a bandage, cloth or just as is.

A fresh herb paste can be applied at affected site. Injectable form: It can also be prepared in the injectable form

Bolus is a large time-release tablet that stays in the rumen of cattle, goats and sheep, etc. bolus can be administered intravenous injection, subcutaneous injection, intramuscular injection, intrathecal, etc.

According to another embodiment of the invention, there is provided a method of treatment of Coccidiosis;

said treatment comprises administration of said herbal medicament of the invention either orally or topically or injectable including intravenous, subcutaneous, etc or by any other suitable route of administration.

According to another embodiment of the invention, there is provided a method of promoting poultry health;

said treatment comprises administration of said herbal poultry supplement of the invention either orally or by any other suitable route of administration.

Particularly, the dosage of herbal medicament is 400 mg per day for seven days for treating Coccidiosis.

Particularly, the dosage of herbal poultry supplement is 400 mg per day for seven day for promoting poultry health.

The present invention is illustrated by the following examples, which is not intended to limit the effective scope of the invention.

Example 1:

Preparation of the composition of the invention

Seeds of Peucedanum grande (400gm), bulbs of Allium, cepa (50 gm) and cloves of Allium satuvum (50 gm) were sieved, washed and sieved followed by drying. All ingredients were grounded to get the homogeneous synergistic composition. The said composition was further converted into pellets and was administered to poultry birds infected with Coccidiosis. The dosage given is 400mg pellets per bird per day.

It was observed that the domestic birds were cured in 7 days.

Medication is employed as preventive care and found poultry were not affected by Coccidiosis. Example 2:

Preparation of the composition of the invention

Seeds of Peucedanum grande (400 gm), bulbs of Allium cepa (50 gm) and cloves of Allium satuvum (50 gm) were sieved, washed and sieved followed by drying. All ingredients were grounded to get the homogeneous synergistic composition. The composition was extracted with 50 ml of mixture of water and alcohol in the ratio of 60:40 at 60°C. The extract was further converted into semisolid composition on removing excess of solvent with the help of rotary evaporator. The composition was further converted into pellets and was administered on poultry birds infected with Coccidiosis. The dosage given is 400mg pellets per bird per day. It was observed that the domestic birds were cured in 4 days.

Medication is employed as preventive care and found poultry were not affected by Coccidiosis. Example 3:

Preparation of the composition of the invention

Seeds of Peucedanum grande (400 gm), bulbs of Allium cepa (50 gm) and cloves of Allium satuvum (50 gm) were sieved, washed and sieved followed by drying separately. Seeds of Peucedanum grande (400 gm), bulbs of Allium cepa (50 gm) and cloves of Allium satuvum (50 gm) were grounded separately to get individual pastes. The pastes so obtained was mixed and - converted into pellets. The pellets were administered on poultry birds infected with Coccidiosis. The dosage given is 400mg pellets per bird per day.

It was observed that the domestic birds were cured in 7 days.

Medication is employed as preventive care and found poultry were not affected by Coccidiosis. Example 4:

Preparation of the composition of the invention

Seeds of Peucedanum grande (400 gm), bulbs of Allium cepa (50 gm) and cloves of Allium satuvum (50 gm) were sieved, washed and sieved separately followed by drying. Seeds of Peucedanum grande (400 gm), bulbs of Allium cepa (50 gm) and cloves of Allium satuvum (50 gm) were grounded separately to get individual pastes. The pastes so obtained were individually extracted with 50 ml mixture of water and alcohol in the ratio of 60: 40 at 60°C. All extracts were combined and converted into semi solid form of composition by removing excess amount of solvent with the help of rotary evaporator. The composition was converted into pellets. The pellets were administered on poultry birds infected with Coccidiosis. The dosage given is 400mg pellets per bird per day.

It was observed that the domestic birds were cured in 4 days.

Medication is employed as preventive care and found poultry were not affected by Coccidiosis.

Example 5:

Comparative example

Seeds of Peucedanum grande (250 gm) were sieved, washed and sieved followed by drying. The Seeds of Peucedanum grande, were grounded to get the smooth paste. The paste was further converted into pellets and was administered on poultry birds infected with Coccidiosis. The dosage given is 400mg per pellets per day.

It was observed that the domestic birds were cured in 10 days.

Example 6:

Comparative example

Seeds of Peucedanum grande (250 gm) were sieved, washed and sieved followed by drying. The Seeds of Peucedanum grande were grounded to get the smooth paste. The paste was extracted with 50 ml of mixture of water and alcohol in the ratio of 60:40 at 60°C. The extract was converted into semi solid form of composition by removing excess amount of solvent with the help of rotary evaporator. The composition was converted into pellets and was administered on poultry birds infected with Coccidiosis. The dosage given is 400mg pellets per day .

It was observed that the poultry was cured in 9 days.

Example 7:

Comparative example

Cloves of Allium satuvum (250 gm) were sieved, washed and sieved followed by drying. The Cloves of Allium satuvum were grounded to get the smooth paste. The paste was further converted into pellets and was administered on poultry birds infected with Coccidiosis. The dosage given is 400mg pellets per day.

It was observed that the poultry was cured in 10 days. Example 8:

Comparative example

Cloves of Allium satuvu (250 gm) were sieved, washed and sieved followed by drying. The Cloves of Allium satuvum were grounded to get the smooth paste. The paste was extracted with 50 ml of mixture of water and alcohol in the ratio of 60: 40 at 60°C. The extract was converted into semi solid form of composition by removing excess amount of solvent with the help of rotary evaporator. The composition was converted into pellets and was administered on poultry birds infected with Coccidiosis. The dosage given is 1 400mg pellets per day.

It was observed that the poultry was cured in 9 days.

Example 9:

Comparative example

Bulbs of Allium cepa (250 gm) were sieved, washed and sieved followed by drying. The Bulbs of Allium cepa were grounded to get the smooth paste. The paste was further converted into pellets and was administered on poultry birds infected with Coccidiosis. The dosage given is 400mg pellets per day.

It was observed that the poultry was cured in 10 days.

Example 10:

Comparative example

Bulbs of Allium cepa (250 gm) were sieved, washed and sieved followed by drying. The Bulbs of Allium cepa were grounded to get the smooth paste. The paste was extracted with 50 ml of mixture of water and alcohol in the ratio of 60 : 40 at 60°C. The extract was converted into semi solid form of composition by removing excess amount of solvent with the help of rotary evaporator. The composition was converted into pellets and was administered on poultry birds infected with Coccidiosis. The dosage given is 400mg per day .

It was observed that the poultry was cured in 9 days.

It was observed that the compositions of the present invention are effective to eradicate Coccidiosis within 4 to 7 days depending upon severity as compared to those of Peucedanum grande, Allium sativum and Allium cepa individually. The present invention as described above, it is to be understood that this invention is not limited to particular methodologies and materials described, as these may vary as per the person skilled in the art. It is also to be understood that the terminology used in the description is for the purpose of describing the particular embodiments only, and is not intended to limit the scope of the present invention.