Title:
HETERODIMERIC PROTEIN COMPOSITION
Document Type and Number:
WIPO Patent Application WO/2014/054804
Kind Code:
A1
Abstract:
The present invention can provide: a heterodimeric protein composition which is a protein comprising a first polypeptide containing an immunoglobulin heavy-chain constant region and a second polypeptide containing a CL-Fc fragment that is a fusion of an immunoglobulin light-chain constant region (CL) and an Fc region, wherein a Cys residue involved in the disulfide bond between the CL region and a CH1 region is deleted or substituted; a method for purifying the protein composition; a method for producing the protein composition; DNA encoding the protein composition; and a vector.
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Inventors:
NIWA RINPEI (JP)
TSUCHIYA MAMI (JP)
MURAKAMI TAKUYA (JP)
TEZUKA YUTA (JP)
TSUCHIYA MAMI (JP)
MURAKAMI TAKUYA (JP)
TEZUKA YUTA (JP)
Application Number:
PCT/JP2013/077172
Publication Date:
April 10, 2014
Filing Date:
October 04, 2013
Export Citation:
Assignee:
KYOWA HAKKO KIRIN CO LTD (JP)
International Classes:
C07K16/46; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/00; C12P21/02
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NATURE, vol. 227, 1970, pages 680
"Current Protocols in Molecular Biology, Antibodies, A Laboratory manual", 1988, COLD SPRING HARBOR LABORATORY, article "Molecular Cloning"
CANCER IMMUNOL. IMMUNOTHER, vol. 36, 1993, pages 373
CANCER IMMUNOL.IMMUNOTHER, vol. 36, 1993, pages 373
AGRIC. BIOL. CHEM., vol. 55, no. 1, 1991, pages 283 - 284
ANAI.BIOCHEM., vol. 171, 1988, pages 73
"Biochemical Experimentation Methods 23- Methods of Studies on Glycoprotein Sugar Chains", 1989, JAPAN SCIENTIFIC SOCIETIES PRESS
J. BIOCHEM., vol. 95, 1984, pages 197
ANAL. BIOCHEM., vol. 171, 1988, pages 73
"Monoclonal Antibodies: Principles and Applications", 1995, WILEY-LISS, INC.
"Enzyme Immunoassay, 3rd Ed.,", 1987, IGAKU-SHOIN LTD.
"Enzyme Antibody Technique", 1985, GAKUSAI KIKAKU
MOL. IMMUNOL., vol. 37, 2000, pages 1035
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J.IMMUNOL. METHODS, vol. 306, 2005, pages 151
PROC. NATL. ACAD. SCI. U.S.A., vol. 89, 1992, pages 4285
SOMATIC CELL MOL.GENET., vol. 12, 1986, pages 555
CATER ET AL., NATURE REVIEW, vol. 6, 2006, pages 343
LABRJIN A F ET AL., CURRENT OPINION IN IMMUNOLOGY, vol. 20, 2008, pages 1
WOLF ET AL., DRUG DISCOVERY TODAY, vol. 10, 2005, pages 1237
KUFER ET AL., TRENDS IN BIOTECH., vol. 22, 2004, pages 238 8
JACKMAN ET AL., J. BIOL. CHEM., vol. 285, 2010, pages 20850
CATER ET AL., J. IMMUNOL. METHODS, vol. 248, 2001, pages 7
RIDWAY ET AL., PROTEIN ENG., vol. 9, 1996, pages 617
MERCHANT ET AL., NATURE BIOTECH., vol. 16, 1998, pages 677
GUNASEKARAN ET AL., J. BIOL. CHEM., vol. 285, 2010, pages 19637
LINDHOFER H ET AL., J. IMMUNOL., vol. 155, 1995, pages 219
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1991, US DEPT. HEALTH AND HUMAN SERVICES
TOMIZUKA K. ET AL., PROC NATL ACAD SCI USA., vol. 97, 2000, pages 722 - 7
WINTER G. ET AL., ANNU REV IMMUNOL., vol. 12, 1994, pages 433 - 55
ROSEN A. ET AL., NATURE, vol. 267, 1977, pages 52 - 54
CANCER IMMUNOL. IMMUNOTHER., vol. 36, 1933, pages 373
ANNU REV. IMMUNOL., vol. 9, 1991, pages 457 - 492
REIKO TAKAHASHI: "Biochemical Experimentation Methods 23-Method for Studying Glycoprotein Sugar Chain", 1989, JAPAN SCIENTIFIC SOCIETIES PRESS
J. LIQ. CHROMATOGR., vol. 6, 1983, pages 1577
"Antibodies, A Laboratory manual", 1988, COLD SPRING HARBOR LABORATORY, article "Molecular Cloning, Second Edition Current protocols in molecular biology"
"Monoclonal Antibodies: principles and practice, Third Edition,", 1993, ACAD. PRESS
"Antibody Engineering, A Practical Approach", 1996, IRL PRESS AT OXFORD UNIVERSITY PRESS
CYTOTECHNOLOGY, vol. 3, 1990, pages 133
NATURE, vol. 329, 1987, pages 840
J. BIOCHEMISTRY, vol. 101, 1987, pages 1307
METHODS IN ENZYMOL., vol. 154, 1987, pages 3
"Molecular Cloning:A Laboratory Manual", 1989, COLD SPRING HARBOR LAB. PRESS
"Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LAB. PRESS
.: "Current Protocols in Molecular Biology", article "Supplement 1-34"
STRATEGIES, vol. 5, 1992, pages 58
NUCLEIC ACIDS RESEARCH, vol. 17, 1989, pages 9494
DNA CLONING: A PRACTICAL APPROACH, vol. I, 1985, pages 49
MOL. CELL. BIOL., vol. 3, 1983, pages 280
GENE, vol. 33, 1985, pages 103
STRATEGIES, vol. 5, 1992, pages 81
GENETICS, vol. 39, 1954, pages 440
SCIENCE, vol. 222, 1983, pages 778
JOURNAL OF MOLECULAR BIOLOGY; J. MOL. BIOL., vol. 166, 1983, pages 1
J. MOL. BIOL., vol. 16, 1966, pages 118
GENE, vol. 38, 1985, pages 275
SANGER ET AL., PROC. NATL. ACAD. SCI., vol. 74, 1977, pages 5463
"Sequences of Proteins of Immunological Interest", 1991, US DEPT. HEALTH AND HUMAN SERVICES
BIO/TECHNOLOGY, vol. 9, 1991, pages 266
J. MOL. BIOL., vol. 112, 1977, pages 535
PROTEIN ENGINEERING, vol. 7, 1994, pages 1501
"Methods in Nucleic Acids Res.", 1991, CRC PRESS, pages: 283
"Methods in Nucleic Acids Res.", 1991, CRC PRESS
PROC. NATL. ACAD. SCI. USA, vol. 84, 1987, pages 7413
"Monoclonal Antibodies-Principles and practice, Third edition,", 1996, ACADEMIC PRESS
"Antibodies-A Laboratory Manual", 1988, COLD SPRING HARBOR LABORATORY
"Manual for Monoclonal Antibody Experiment", 1987, KODANSHA SCIENTIFIC LTD
PROC. NATL. ACAD. SCI. U.S.A., vol. 84, 1987, pages 7413
TAKASHI YOKOTA AND KENICHI ARAI: "Biomanual Series 4-Methods of Gene Transfer, Expression and Analysis", 1994, YODOSHA
PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216
SOMATIC CELL AND MOLECULAR GENETICS, vol. 12, 1986, pages 55
METHODS ENZYMOL., vol. 194, 1990, pages 182
PROC. NATL. ACAD. SCI. USA, vol. 84, 1978, pages 1929
J. BACTERIOLOGY, vol. 153, 1983, pages 163
PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929
"current Protocols in Molecular Biology; Baculovirus Expression Vectors, A Laboratory Manual", 1992, W.H.FREEMAN AND COMPANY
BIO/TECHNOLOGY, vol. 6, 1988, pages 47
AMERICAN JOURNAL OF CLINICAL NUTRITION, vol. 63, 1996, pages 639S
AMERICAN JOURNAL OF CLINICAL NUTRITION, vol. 63, 1996, pages 627S
BIO/TECHNOLOGY, vol. 9, 1991, pages 830
TISSUE CULTURE, 1994, pages 20
TISSUE CULTURE, 1995, pages 21
TRENDS IN BIOTECHNOLOGY, vol. 15, 1997, pages 45
NATURE, vol. 227, 1970, pages 680
"Current Protocols in Molecular Biology, Antibodies, A Laboratory manual", 1988, COLD SPRING HARBOR LABORATORY, article "Molecular Cloning"
CANCER IMMUNOL. IMMUNOTHER, vol. 36, 1993, pages 373
CANCER IMMUNOL.IMMUNOTHER, vol. 36, 1993, pages 373
AGRIC. BIOL. CHEM., vol. 55, no. 1, 1991, pages 283 - 284
ANAI.BIOCHEM., vol. 171, 1988, pages 73
"Biochemical Experimentation Methods 23- Methods of Studies on Glycoprotein Sugar Chains", 1989, JAPAN SCIENTIFIC SOCIETIES PRESS
J. BIOCHEM., vol. 95, 1984, pages 197
ANAL. BIOCHEM., vol. 171, 1988, pages 73
"Monoclonal Antibodies: Principles and Applications", 1995, WILEY-LISS, INC.
"Enzyme Immunoassay, 3rd Ed.,", 1987, IGAKU-SHOIN LTD.
"Enzyme Antibody Technique", 1985, GAKUSAI KIKAKU
MOL. IMMUNOL., vol. 37, 2000, pages 1035
J. IMMUNOL. METHODS, vol. 306, 2005, pages 151
J.IMMUNOL. METHODS, vol. 306, 2005, pages 151
PROC. NATL. ACAD. SCI. U.S.A., vol. 89, 1992, pages 4285
SOMATIC CELL MOL.GENET., vol. 12, 1986, pages 555
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HAMADA Yuriko et al. (JP)
Yuriko Hamada (JP)
Yuriko Hamada (JP)
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