Title:
HETEROLOGOUS INTRON WITHIN A SIGNAL PEPTIDE
Document Type and Number:
WIPO Patent Application WO/2014/102103
Kind Code:
A3
Abstract:
The invention concerns the field of recombinant gene engineering. It concerns novel introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes with heterologous introns, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel intron sequences as heterologous introns.
Inventors:
ENENKEL BARBARA (DE)
Application Number:
PCT/EP2013/077042
Publication Date:
August 28, 2014
Filing Date:
December 18, 2013
Export Citation:
Assignee:
BOEHRINGER INGELHEIM INT (DE)
International Classes:
C12N15/13; C12N5/10; C12N15/62; C12N15/63; C12N15/67; C12N15/85; C12P21/00; C07K16/00
Domestic Patent References:
| WO2007045465A1 | 2007-04-26 | |||
| WO2009055656A2 | 2009-04-30 | |||
| WO2006071804A2 | 2006-07-06 | |||
| WO2002088307A2 | 2002-11-07 | |||
| WO2010010108A1 | 2010-01-28 |
Other References:
NEUBERGER M S: "Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.", THE EMBO JOURNAL 1983, vol. 2, no. 8, 1983, pages 1373 - 1378, XP002713612, ISSN: 0261-4189
NEUBERGER M S ET AL: "The intron requirement for immunoglobulin gene expression is dependent upon the promoter.", NUCLEIC ACIDS RESEARCH 25 JUL 1988, vol. 16, no. 14B, 25 July 1988 (1988-07-25), pages 6713 - 6724, XP002713613, ISSN: 0305-1048
BUCHMAN A R ET AL: "COMPARISON OF INTRON-DEPENDENT AND INTRON-INDEPENDENT GENE EXPRESSION", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 8, no. 10, 1 October 1988 (1988-10-01), pages 4395 - 4405, XP001079759, ISSN: 0270-7306
NEUBERGER M S ET AL: "The intron requirement for immunoglobulin gene expression is dependent upon the promoter.", NUCLEIC ACIDS RESEARCH 25 JUL 1988, vol. 16, no. 14B, 25 July 1988 (1988-07-25), pages 6713 - 6724, XP002713613, ISSN: 0305-1048
BUCHMAN A R ET AL: "COMPARISON OF INTRON-DEPENDENT AND INTRON-INDEPENDENT GENE EXPRESSION", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 8, no. 10, 1 October 1988 (1988-10-01), pages 4395 - 4405, XP001079759, ISSN: 0270-7306
Attorney, Agent or Firm:
SIMON, Elke et al. (Corporate PatentsBinger Strasse 173, Ingelheim am Rhein, DE)
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