Title:
HIERARCHICAL MODEL FOR DETECTING BENIGN AND MALIGNANT DEGREES OF COLORECTAL TUMORS AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/042380
Kind Code:
A1
Abstract:
The present invention relates to a hierarchical model for detecting colorectal tumors and an application thereof, wherein the model grades the change of an imprinted gene in a colorectal tumor by means of calculating the defect expression amount of the imprinted gene, copy number variation expression amount of the imprinted gene and total expression amount of the imprinted gene. The described detection model and device visually express the performance of imprinting defect in the tissue and cell samples of a colorectal tumor patient, and detect the change in an imprinted gene objectively, visually, early and accurately by means of the method of in situ labeling for the imprinted gene. In addition, the detection model and device may provide quantitative models, which makes a significant contribution to the diagnosis of colorectal tumors.
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Inventors:
CHENG TONG (CN)
ZHOU NING (CN)
ZHOU NING (CN)
Application Number:
PCT/CN2018/103357
Publication Date:
March 07, 2019
Filing Date:
August 30, 2018
Export Citation:
Assignee:
LISEN IMPRINTING DIAGNOSTICS INC (US)
LI XING (CN)
LI XING (CN)
International Classes:
G01N33/68; G01N1/30; G01N33/574
Foreign References:
| CN103344762A | 2013-10-09 |
Other References:
NISHIHARA, S.: "Multipoint imprinting analysis in sporadic colorectal can- cers with and without microsatellite instability", INTERNATIONAL JOURNAL OF ONCOLOGY, 1 August 2000 (2000-08-01), XP055579749
SILVIERA, M.L.: "Epigenetic Differences in Normal Colon Mucosa of Cancer Patients Suggest Altered Dietary Metabolic Pathways", CANCER PREV. RES., vol. 5, no. 3, 1 March 2012 (2012-03-01), pages 374 - 384, XP055581036
CALON, A.: "Stromal gene expression defines poor-prognosis subtypes in co- lorectal cancer", NATURE GENETICS, vol. 47, no. 4, 23 February 2015 (2015-02-23), pages 320 - 329, XP055297456, DOI: doi:10.1038/ng.3225
IINO, H.: "DNA microsatellite instability and mismatch repair protein loss in adenomas presenting in hereditary non-polyposis colorectal cancer", GUT, vol. 47, no. 1, 1 July 2000 (2000-07-01) - 42, pages 37, XP055581043
KOWALCZYK, A.E. ET AL.: "Altered expression of the PLAGL1 (ZAC1/LOT1) gene in co- lorectal cancer: Correlations to the clinicopathological parameters", INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 47, no. 3, 30 September 2015 (2015-09-30), pages 951 - 962, XP055578711
ORTEGA P. ET AL: "MMP-7 and SGCE as distinctive molecular factors in sporadic colorectal cancers from the mutator phenotype pathway", INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 36, no. 5, 1 May 2010 (2010-05-01), pages 1209 - 1215, XP055555564
BREG, J. S.: "Imprinted Genes That Regulate Early Mammalian Growth Are Coe- xpressed in Somatic Stem Cells", PLOS ONE, vol. 6, no. 10, 19 October 2011 (2011-10-19), pages e26410, XP055581051
See also references of EP 3726221A4
SILVIERA, M.L.: "Epigenetic Differences in Normal Colon Mucosa of Cancer Patients Suggest Altered Dietary Metabolic Pathways", CANCER PREV. RES., vol. 5, no. 3, 1 March 2012 (2012-03-01), pages 374 - 384, XP055581036
CALON, A.: "Stromal gene expression defines poor-prognosis subtypes in co- lorectal cancer", NATURE GENETICS, vol. 47, no. 4, 23 February 2015 (2015-02-23), pages 320 - 329, XP055297456, DOI: doi:10.1038/ng.3225
IINO, H.: "DNA microsatellite instability and mismatch repair protein loss in adenomas presenting in hereditary non-polyposis colorectal cancer", GUT, vol. 47, no. 1, 1 July 2000 (2000-07-01) - 42, pages 37, XP055581043
KOWALCZYK, A.E. ET AL.: "Altered expression of the PLAGL1 (ZAC1/LOT1) gene in co- lorectal cancer: Correlations to the clinicopathological parameters", INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 47, no. 3, 30 September 2015 (2015-09-30), pages 951 - 962, XP055578711
ORTEGA P. ET AL: "MMP-7 and SGCE as distinctive molecular factors in sporadic colorectal cancers from the mutator phenotype pathway", INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 36, no. 5, 1 May 2010 (2010-05-01), pages 1209 - 1215, XP055555564
BREG, J. S.: "Imprinted Genes That Regulate Early Mammalian Growth Are Coe- xpressed in Somatic Stem Cells", PLOS ONE, vol. 6, no. 10, 19 October 2011 (2011-10-19), pages e26410, XP055581051
See also references of EP 3726221A4
Attorney, Agent or Firm:
BEYOND ATTORNEYS AT LAW (CN)
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