Title:
HIGH AMYLOSE WHEAT - IV
Document Type and Number:
WIPO Patent Application WO/2019/136518
Kind Code:
A1
Abstract:
The present invention provides wheat grain of the species Triticum
aestivum, the grain comprising i) mutations in each of its SSIIa genes such that the grain is homozygous for a mutation in its SSIIa-A gene, homozygous for a mutation in its SSIIa-B gene and homozygous for a mutation in its SSIIa-D gene, wherein at least two of the mutations in said SSIIa genes are null mutations, mutations in each of its SSIIIa genes such that the grain is homozygous for a mutation in its SSIIIa-A gene, homozygous for a mutation in its SSIIIa-B gene and homozygous for a mutation in its SSIIIa-D gene, wherein at least two of the mutations in said SSIIIa genes are null mutations ii) a total starch content comprising an amylose content and an amylopectin content, iii) a fructan content which is increased relative to wild-type wheat grain on a weight basis, preferably between 3% and 12% of the grain weight, iv) a β-glucan content, v) an arabinoxylan content, vi) a cellulose content. The grain has a weight of between 25mg and 60mg, and the amylose content is between 45% and 70% on a weight basis of the total starch content of the grain as determined by iodine binding assay. The amylopectin content on a weight basis is reduced relative to the wild-type wheat grain, and each of the β-glucan content, arabinoxylan content and cellulose content are increased relative to the wild-type wheat grain on a weight basis, such that the sum of the fructan content, β-glucan content, arabinoxylan content and cellulose content is between 15% and 30% of the grain weight.
Inventors:
LI ZHONGYI (AU)
Application Number:
PCT/AU2019/050008
Publication Date:
July 18, 2019
Filing Date:
January 10, 2019
Export Citation:
Assignee:
ARISTA CEREAL TECH PTY LTD (AU)
International Classes:
C12N15/52; A01H1/06; A01H5/10; A23L29/212; A61K31/718; C12N9/10
Domestic Patent References:
| WO2012103594A1 | 2012-08-09 | |||
| WO2017008001A1 | 2017-01-12 | |||
| WO2000066745A1 | 2000-11-09 | |||
| WO2018006126A1 | 2018-01-11 | |||
| WO2018092155A1 | 2018-05-24 |
Other References:
HOGG, A.C. ET AL.: "Novel ssIIa alleles produce specific seed amylose levels in hexaploid wheat", CEREAL CHEMISTRY, vol. 94, 2017, pages 1008 - 1015, XP055727170
KON1K-ROSE, C. ET AL.: "Effects of starch synthase IIa gene dosage on grain, protein and starch in endosperm of wheat", THEORETICAL AND APPLIED GENETICS, vol. 115, 2007, pages 1053 - 1065, XP019558602, DOI: 10.1007/s00122-007-0631-0
CHENG, ZHIJUN; ZHANG, XIN; GUO, XIUPING; SU, NING; JIANG, LING; MAO, LONG; WAN, JIANMIN: "Double repression of soluble starch synthase genes SSIIa and SSIIIa in rice (Oryza sativa L.) uncovers interactive effects on the physicochemical properties of starch", GENOME, vol. 54, no. 6, 2011, pages 448 - 459, XP009522687, DOI: 10.1139/g11-010
YAMAMORI, M. ET AL.: "Genetic elimination of a starch granule protein, SGP-1, of wheat generates an altered starch with apparent high amylose", THEORETICAL AND APPLIED GENETICS, vol. 101, 2000, pages 21 - 29, XP001084945, DOI: 10.1007/s001220051444
HAYASHI, M. ET AL.: "Analyses of starch biosynthetic protein complexes and starch properties from developing mutant rice seeds with minimal starch synthase activities", BMC PLANT BIOLOGY, vol. 18, 2018, pages 59, XP055727198
See also references of EP 3737764A4
KON1K-ROSE, C. ET AL.: "Effects of starch synthase IIa gene dosage on grain, protein and starch in endosperm of wheat", THEORETICAL AND APPLIED GENETICS, vol. 115, 2007, pages 1053 - 1065, XP019558602, DOI: 10.1007/s00122-007-0631-0
CHENG, ZHIJUN; ZHANG, XIN; GUO, XIUPING; SU, NING; JIANG, LING; MAO, LONG; WAN, JIANMIN: "Double repression of soluble starch synthase genes SSIIa and SSIIIa in rice (Oryza sativa L.) uncovers interactive effects on the physicochemical properties of starch", GENOME, vol. 54, no. 6, 2011, pages 448 - 459, XP009522687, DOI: 10.1139/g11-010
YAMAMORI, M. ET AL.: "Genetic elimination of a starch granule protein, SGP-1, of wheat generates an altered starch with apparent high amylose", THEORETICAL AND APPLIED GENETICS, vol. 101, 2000, pages 21 - 29, XP001084945, DOI: 10.1007/s001220051444
HAYASHI, M. ET AL.: "Analyses of starch biosynthetic protein complexes and starch properties from developing mutant rice seeds with minimal starch synthase activities", BMC PLANT BIOLOGY, vol. 18, 2018, pages 59, XP055727198
See also references of EP 3737764A4
Attorney, Agent or Firm:
DAVIES COLLISON CAVE PTY LTD (AU)
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