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Title:
HIGH CAMP YIELDING YEAST STRAIN AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/139959
Kind Code:
A1
Abstract:
Disclosed are a high cyclic adenosine monophosphate (cAMP) yielding yeast strain and a construction method therefor. The method comprises constructing Saccharomyces cerevisiae deficient in both protein kinase A catalytic subunit encoding genes TPK1, TPK2, TPK3 and protein kinase encoding gene YAK1. The method also comprises further deleting phosphodiesterase encoding gene PDE1 and the encoding genes of transcription factors Bas1 and Bas2 in the yeast strain on the basis of the deletion of the four genes mentioned above. The recombinant yeast strain can produce extracellular cAMP more efficiently in relation to a yeast strain without genetic modifications.

Inventors:
ZOU, Shaolan (Tianjin University, No. 85 BaiWan Rd. Jinnan District, Tianjin 2, 300072, CN)
GAO, Wenxuan (Tianjin University, No. 85 BaiWan Rd. Jinnan District, Tianjin 2, 300072, CN)
HU, Yun (Tianjin University, No. 85 BaiWan Rd. Jinnan District, Tianjin 2, 300072, CN)
YANG, Shunhua (Tianjin University, No. 85 BaiWan Rd. Jinnan District, Tianjin 2, 300072, CN)
MA, Pingsheng (Tianjin University, No. 85 BaiWan Rd. Jinnan District, Tianjin 2, 300072, CN)
Application Number:
CN2016/074067
Publication Date:
August 24, 2017
Filing Date:
February 18, 2016
Export Citation:
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Assignee:
TIANJIN UNIVERSITY (Tianjin University, 92 Wei Jin Rd. Tian Jin, China, Tianjin 2, 300072, CN)
International Classes:
C12N15/01; C12N1/16; C12R1/645
Other References:
JI, XIAOBING: "Several Ways to Make Saccharomyces Cerevisiae Produce High Yield of cAMP (Registration of Postgraduate Academic Report", E-LEARNING PLATFORM, 31 October 2012 (2012-10-31)
LIVASE, D. ET AL.: "Transcriptional Responses to Glucose in Saccharomyces Cerevisiae Strains Lacking A Functional Protein Kinase A", BMC GENOMICS, vol. 12, no. 405, 9 August 2011 (2011-08-09), XP021091853
ZHAO, HUANSHENG: "Comparison of the Activity of the PKA Catalytic Subunits in S.cerevisiae", BASIC SCIENCES, CHINA MASTER'S THESES FULL-TEXT DATABASE, 15 August 2009 (2009-08-15)
PAN, XUEWEN ET AL.: "Cyclic AMP-Dependent Protein Kinase Regulates Pseudohyphal Differentiation in Saccharomyces Cerevisiae", MOL CELL BIOL, vol. 19, no. 7, 31 July 1999 (1999-07-31), XP055409430
ZURITA-MARTINEZ, S.A. ET AL.: "Tor and Cyclic AMP-Protein Kinase A: Two Parallel Pathways Regulating Expression of Genes Required for Cell Growth", EUKARYOTIC CELL, vol. 4, no. 1, 31 January 2005 (2005-01-31), XP055409431
SOM, I. ET AL.: "DNA-Bound Basl Recruits Pho2 to Activate ADE Genes in Saccharomyces Cerevisiae", EUKARYOTIC CELL, vol. 4, no. 1, 31 October 2005 (2005-10-31), XP055409432
Attorney, Agent or Firm:
BEIJING LINKAW PATENT ATTORNEY LAW FIRM (CHEN, chaoRm.309, Unit 2, Apt.6, No.1 Beiqing Road, Huilongguan Town,Changping District, Beijing 6, 102206, CN)
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