Title:
HIGH-EFFICIENCY METHOD FOR LINKING DNA CONNECTOR
Document Type and Number:
WIPO Patent Application WO/2018/006567
Kind Code:
A1
Abstract:
Provided is a high-efficiency method for linking a connector, the method comprising: using a type A DNA polymerase or a DNA polymerase Klenow fragment to process a DNA molecule so as to add a 3'dA protruding end; then linking the processed DNA molecule to a connector mixture to obtain a linking product, wherein the connector mixture comprises three kinds of double-strand DNA connectors, and the ends of the three double-strand DNA connectors are a blunt end, a 3'dT protruding end and a 3'dC protruding end respectively. The method may effectively improve the efficiency of linking a connector to DNA.
Inventors:
GENG LIANG (CN)
XIN WEN (CN)
XIN WEN (CN)
Application Number:
PCT/CN2016/113506
Publication Date:
January 11, 2018
Filing Date:
December 30, 2016
Export Citation:
Assignee:
BEIJING TRANSGEN BIOTECH CO LTD (CN)
International Classes:
C12N15/10
Domestic Patent References:
| WO2012013932A1 | 2012-02-02 | |||
| WO2015103339A1 | 2015-07-09 |
Foreign References:
| CN105950612A | 2016-09-21 | |||
| CN102061335A | 2011-05-18 | |||
| CN104789553A | 2015-07-22 |
Other References:
MAGNUSON, V.L. ET AL.: "Substrate Nucleotide-determined Non-templated Addition of Adenine by Tag DNA Polymerase: Implications for PCR-based Genotyping and Cloning", BIOTECHNIQUES, vol. 21, no. 4, 31 October 1996 (1996-10-31), pages 700 - 709, XP008062075, ISSN: 0736-6205
Attorney, Agent or Firm:
BEIJING JANLEA PATENT AGENCY CO., LTD. (CN)
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