Title:
HIGH-EFFICIENCY THROMBIN FROM REPTILES AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/253695
Kind Code:
A1
Abstract:
Provided is a thrombin from reptiles, which is obtained by means of the following method: using a gene cloning method to obtain a sequence of Gekko japonicus Prethrombin-2; constructing a Prethrombin-2 eukaryotic expression recombinant plasmid, transfecting 293T cells, and inducing a Prethrombin-2 recombinant protein to be expressed and then purifying same; and using Ecarin to activate the Prethrombin-2 recombinant protein so as to obtain Gecko Thrombin (gTm) having a thrombin activity.
Inventors:
WANG YONGJUN (CN)
WANG YINGJIE (CN)
LIANG HAO (CN)
WANG YINGJIE (CN)
LIANG HAO (CN)
Application Number:
PCT/CN2020/121437
Publication Date:
December 23, 2021
Filing Date:
October 16, 2020
Export Citation:
Assignee:
UNIV NANTONG (CN)
International Classes:
A61K38/48; C12N9/74; A61P7/04; A61P9/00; A61P17/02
Domestic Patent References:
| WO2020008079A1 | 2020-01-09 |
Foreign References:
| CN111593037A | 2020-08-28 | |||
| CN109207462A | 2019-01-15 |
Other References:
DATABASE Protein 20 January 2016 (2016-01-20), ANONYMOUS: "PREDICTED:Prothrombin[Gekko japonicus]", XP055882278, retrieved from genbank Database accession no. XP_015262498
MÁRCIA B. SILVA; MIRTA SCHATTNER; CELSO R.R. RAMOS; INÁCIO L.M. JUNQUEIRA-DE-AZEVEDO; MÍRIAM C. GUARNIERI; MARÍA A. LAZZARI; CLAUD: "A prothrombin activator from Bothrops erythromelas (jararaca-da-seca) snake venom: characterization and molecular cloning", BIOCHEMICAL JOURNAL, vol. 369, no. 1, 1 January 2003 (2003-01-01), GB , pages 129 - 139, XP009533172, ISSN: 0264-6021, DOI: 10.1042/bj20020449
MÁRCIA B. SILVA; MIRTA SCHATTNER; CELSO R.R. RAMOS; INÁCIO L.M. JUNQUEIRA-DE-AZEVEDO; MÍRIAM C. GUARNIERI; MARÍA A. LAZZARI; CLAUD: "A prothrombin activator from Bothrops erythromelas (jararaca-da-seca) snake venom: characterization and molecular cloning", BIOCHEMICAL JOURNAL, vol. 369, no. 1, 1 January 2003 (2003-01-01), GB , pages 129 - 139, XP009533172, ISSN: 0264-6021, DOI: 10.1042/bj20020449
Attorney, Agent or Firm:
NANJING IPLEADER INTELLECTUAL PROPERTY AGENCY FIRM (CN)
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