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Title:
HIGH LYSINE MAIZE COMPOSITIONS AND METHODS FOR DETECTION THEREOF
Document Type and Number:
WIPO Patent Application WO2005061720
Kind Code:
A3
Abstract:
Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.

Inventors:
DIZIGAN MARK ANTHONY (US)
VOYLES DALE A (US)
MALLOY KATHLEEN P (US)
KELLY REBECCA A (US)
MALVAR THOMAS (US)
LUETHY MICHAEL HANS (US)
Application Number:
PCT/US2004/040586
Publication Date:
March 30, 2006
Filing Date:
December 03, 2004
Export Citation:
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Assignee:
MONSANTO TECHNOLOGY LLC (US)
DIZIGAN MARK ANTHONY (US)
VOYLES DALE A (US)
MALLOY KATHLEEN P (US)
KELLY REBECCA A (US)
MALVAR THOMAS (US)
LUETHY MICHAEL HANS (US)
International Classes:
A01H1/00; A01H5/10; A23L7/10; C12N15/82; (IPC1-7): C12N15/82; A01H1/00
Domestic Patent References:
WO2000063401A12000-10-26
Foreign References:
US6329574B12001-12-11
Other References:
MCELROY ET AL: "Isolation of an efficient actin promoter for use in rice transformation", February 1990, PLANT CELL, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, US, PAGE(S) 163-171, ISSN: 1040-4651, XP002118812
FALCO S C ET AL: "TRANSGENIC CANOLA AND SOYBEAN SEEDS WITH INCREASED LYSINE", June 1995, BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, PAGE(S) 577-582, ISSN: 0733-222X, XP002035500
DATABASE EMBL [online] 20 June 2003 (2003-06-20), "OGLBU75TV ZM_0.7_1.5_KB Zea mays genomic clone ZMMBMa0318N05, genomic survey sequence.", XP002345080, retrieved from EBI accession no. EM_GSS:CC633189 Database accession no. CC633189
DATABASE EMBL [online] 28 August 2003 (2003-08-28), WHITELAW: "Zea maize genomic clone", XP002345081, retrieved from EBI Database accession no. CG054145
DATABASE EMBL [online] WHITELAW C.A.: "Zea maize genomic clone", XP002345082, retrieved from EBI Database accession no. CG176305
DATABASE EMBL [online] 6 February 2003 (2003-02-06), WHITELAW C.A.: "Zea maize genomic clone", XP002345083, retrieved from EBI Database accession no. BZ681205
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