HIGH-MIGRATION MESENCHYMAL STEM CELL, AND PREPARATION METHOD THEREFOR AND APPLICATION THEREOF

Title:

HIGH-MIGRATION MESENCHYMAL STEM CELL, AND PREPARATION METHOD THEREFOR AND APPLICATION THEREOF

Document Type and Number:

WIPO Patent Application WO/2022/213704

Kind Code:

A1

Abstract:

Provided are a high-migration mesenchymal stem cell, and a preparation method therefor and an application thereof. The preparation method comprises: inoculating and suspending mesenchymal stem cells in a mesenchymal stem cell culture solution, carrying out flowing liquid culture in a cell culture roller bottle or a stirred fermentation tank without adding a microcarrier in the culture process, collecting cell spheroids after culture is finished, washing and digesting to form single cells to obtain high-migration mesenchymal stem cells. The high-migration mesenchymal stem cells prepared have a small size, an average diameter of 11-14 μm, an expression level of CXCR4 on the cell surfaces up to 30% or above, and a strong cell migration ability, and can basically prevent retention in lungs when being intravenously injected, and the number of the mesenchymal stem cells reaching ischemic myocardium is remarkably increased.

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Inventors:

WU YAOJIONG (CN)
MO MIAOHUA (CN)

Application Number:

PCT/CN2022/072878

Publication Date:

October 13, 2022

Filing Date:

January 20, 2022

Export Citation:

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Assignee:

TSINGHUA SHENZHEN INT GRADUATE SCHOOL (CN)

International Classes:

C12N5/0775; A61K35/28; A61P9/10; A61P25/28; A61P29/00; A61P37/02; A61P37/06; A61P43/00

Foreign References:

CN113106059A	2021-07-13
CN103667186A	2014-03-26
CN102317448A	2012-01-11
CN111518762A	2020-08-11
CN101735980A	2010-06-16
US20140038291A1	2014-02-06

Other References:

WANG YUNSHUAI, TAO CHEN,HONGJIE YAN,HUI QI,CHUNYAN DENG,TAO YE,SHUYAN ZHOU,FU-RONG LI: "Role of Histone Deacetylase Inhibitors in the Aging of Human Umbilical Cord Mesenchymal Stem Cells", JOURNAL OF CELLULAR BIOCHEMISTRY, JOHN WILEY & SONS, INC. JOHN WILEY & SONS, INC., HOBOKEN, USA, vol. 114, no. 10, 8 April 2013 (2013-04-08), Hoboken, USA, pages 2231 - 2239, XP055974883, ISSN: 0730-2312, DOI: 10.1002/jcb.24569
XIAO-BO ZHU; ZHENG-XIN LI; XIN-XIN GU; ZHUO LEI; JIE ZHANG; HAI-TAO LI; MING-MING ZHOU: "Trichostatin A combined with cytokines induces differentiation of embryonic stem cells into hepatocytes", WORLD CHINESE JOURNAL OF DIGESTOLOGY, SHIJE WEI-CHANGBINGXUE ZAZHISHE , TAIYUAN, CN, vol. 23, no. 8, 18 March 2015 (2015-03-18), CN , pages 1278 - 1284, XP009540272, ISSN: 1009-3079

Attorney, Agent or Firm:

BEIJING SANYOU INTELLECTUAL PROPERTY AGENCY LTD. (CN)

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