HIGH-PURITY D-PSICOSE PREPARATION METHOD - SHANDONG BAILONG CHUANGYUAN BIO TECH CO LTD

Title:

HIGH-PURITY D-PSICOSE PREPARATION METHOD

Document Type and Number:

WIPO Patent Application WO/2018/099479

Kind Code:

A1

Abstract:

A preparation method for high purity D-psicose, having the following steps: (1) after centrifuging a fermentation broth of Bacillus subtilis, extracted bacteria going through homogenization processing, obtaining a mixed liquor containing D-psicose 3-epimerase; (2) formulating a fructose solution and adding the solution to the mixed liquor containing D-psicose 3-epimerase, adjusting pH value, adding cobalt chloride, performing a preserved-heat reaction, then feeding fructose solution into the reaction liquid, continuing the reaction, and stopping the reaction, obtaining crude D-psicose liquid; (3) after performing decoloring, filtering, ion exchange, chromatographic separation and concentration on the crude D-psicose liquid, crystallizing or drying the liquid, obtaining D-psicose.

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Inventors:

DOU BAODE (CN)
DOU GUANGPENG (CN)
ZHANG MINGZHAN (CN)
SHAO XIANBAO (CN)
LI FANGHUA (CN)
DU QIAN (CN)
YANG TENGTENG (CN)

Application Number:

PCT/CN2017/114289

Publication Date:

June 07, 2018

Filing Date:

December 01, 2017

Export Citation:

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Assignee:

SHANDONG BAILONG CHUANGYUAN BIO TECH CO LTD (CN)

International Classes:

C12N9/90; C12P19/02; C12P19/24

Foreign References:

CN106520746A	2017-03-22
CN105802897A	2016-07-27
CN102869783A	2013-01-09
CN103333935A	2013-10-02
CN106164265A	2016-11-23

Attorney, Agent or Firm:

JINAN JINDI INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)

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