Title:
HIGH RESOLUTION DUAL-OBJECTIVE MICROSCOPY
Document Type and Number:
WIPO Patent Application WO/2013/090360
Kind Code:
A3
Abstract:
The present invention generally relates to super-resolution microscopy. For example, certain aspects of the invention are generally directed to a microscopy system comprising at least two objectives. In some embodiments, the microscopy system may also contain a non-circularly- symmetric lens. One or more images can be obtained using the objectives, for example, using stochastic imaging techniques such as STORM (stochastic optical reconstruction microscopy), optionally in conjunction with entities that are photoactivatable and/or photo switchable. The images obtained using the objectives may be compared, e.g., to remove noise, and/or to compare an entity present in both images, for instance, to determine the z-position of the entity. In some cases, surprisingly high resolutions may be obtained using such techniques, for example, resolutions of better than about 10 nm.
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Inventors:
ZHUANG XIAOWEI (US)
XU KE (US)
BABCOK HAZEN P (US)
XU KE (US)
BABCOK HAZEN P (US)
Application Number:
PCT/US2012/069138
Publication Date:
August 15, 2013
Filing Date:
December 12, 2012
Export Citation:
Assignee:
HARVARD COLLEGE (US)
International Classes:
G02B21/16; G02B21/36; G02B27/58
Domestic Patent References:
| WO2009085218A1 | 2009-07-09 |
Foreign References:
| US20100231922A1 | 2010-09-16 |
Other References:
G. SHTENGEL ET AL: "Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 106, no. 9, 3 March 2009 (2009-03-03), pages 3125 - 3130, XP055007791, ISSN: 0027-8424, DOI: 10.1073/pnas.0813131106
Attorney, Agent or Firm:
OYER, Timothy, J. (Greenfield & Sacks P.C.,600 Atlantic Avenu, Boston MA, US)
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