Title:
HIGH-SPEED MATURATION METHOD FOR AN OLIGONUCLEOTIDE LIBRARY FOR THE PURPOSE OF PREPARING A PROTEIN LIBRARY
Document Type and Number:
WIPO Patent Application WO/2011/162355
Kind Code:
A1
Abstract:
The disclosed method for manufacturing a matured oligonucleotide library includes: a step in which an oligonucleotide library to be matured is terminally modified, with a tag sequence added to the 5' end and an arrest sequence that stops ribosome translation elongation added to the 3' end; a step in which the terminally modified sequence is transcribed, yielding a transcript; and an in vitro translation step in which said transcript is translated in vitro. In said method, the oligonucleotide library to be matured is a random oligonucleotide library.
Inventors:
UEDA TAKUYA (JP)
KANAMORI TAKASHI (JP)
KOJOH KANEHISA (JP)
KATOH SHIZUE (JP)
MIYAKOSHI AKIRA (JP)
KANAMORI TAKASHI (JP)
KOJOH KANEHISA (JP)
KATOH SHIZUE (JP)
MIYAKOSHI AKIRA (JP)
Application Number:
PCT/JP2011/064455
Publication Date:
December 29, 2011
Filing Date:
June 23, 2011
Export Citation:
Assignee:
UNIV TOKYO (JP)
GENEFRONTIER CORP (JP)
UEDA TAKUYA (JP)
KANAMORI TAKASHI (JP)
KOJOH KANEHISA (JP)
KATOH SHIZUE (JP)
MIYAKOSHI AKIRA (JP)
GENEFRONTIER CORP (JP)
UEDA TAKUYA (JP)
KANAMORI TAKASHI (JP)
KOJOH KANEHISA (JP)
KATOH SHIZUE (JP)
MIYAKOSHI AKIRA (JP)
International Classes:
C12N15/09
Foreign References:
| JP2009112286A | 2009-05-28 | |||
| JP2007029061A | 2007-02-08 | |||
| JP2008271903A | 2008-11-13 | |||
| JP2008271903A | 2008-11-13 |
Other References:
OSADA E. ET AL.: "Epitope mapping using ribosome display in a reconstituted cell-free protein synthesis system", THE JOURNAL OF BIOCHEMISTRY, vol. 145, no. 5, 2009, pages 693 - 700, XP002677147
NAKATOGAWA; ITO, CELL, vol. 108, 2002, pages 629 - 636
GONG ET AL., SCIENCE, vol. 297, 2002, pages 1864 - 1867
TANNER ET AL., J. BIOL. CHEM, vol. 284, 2009, pages 34809 - 34818
HOOD ET AL., ANNU. REV. MICROBIOL, vol. 63, 2009, pages 385 - 409
SHIMIZU Y; INOUE A; TOMARI Y; SUZUKI T; YOKOGAWA T; NISHIKAWA K; UEDA T: "Cell-free translation reconstituted with purified components", NATURE BIOTECHNOLOGY, vol. 19, 2001, pages 751 - 755
VILLEMAGNE ET AL., J. IMMUNOL. METHODS, vol. 313, 2006, pages 140 - 148
MOORE; SAUER, ANNU. REV. BIOCHEM., vol. 76, 2007, pages 101 - 124
HANES ET AL., PROC. NATL. ACAD. SCI. USA, vol. 94, 1997, pages 4937 - 4942
SHIMIZU ET AL., METHODS, vol. 36, 2005, pages 299 - 304
See also references of EP 2586863A4
NAKATOGAWA; ITO, CELL, vol. 108, 2002, pages 629 - 636
GONG ET AL., SCIENCE, vol. 297, 2002, pages 1864 - 1867
TANNER ET AL., J. BIOL. CHEM, vol. 284, 2009, pages 34809 - 34818
HOOD ET AL., ANNU. REV. MICROBIOL, vol. 63, 2009, pages 385 - 409
SHIMIZU Y; INOUE A; TOMARI Y; SUZUKI T; YOKOGAWA T; NISHIKAWA K; UEDA T: "Cell-free translation reconstituted with purified components", NATURE BIOTECHNOLOGY, vol. 19, 2001, pages 751 - 755
VILLEMAGNE ET AL., J. IMMUNOL. METHODS, vol. 313, 2006, pages 140 - 148
MOORE; SAUER, ANNU. REV. BIOCHEM., vol. 76, 2007, pages 101 - 124
HANES ET AL., PROC. NATL. ACAD. SCI. USA, vol. 94, 1997, pages 4937 - 4942
SHIMIZU ET AL., METHODS, vol. 36, 2005, pages 299 - 304
See also references of EP 2586863A4
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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