Title:
HIGH-THROUGHPUT AUTOMATED GENE SYNTHESIS APPARATUS BASED ON CLUSTER ARRAY
Document Type and Number:
WIPO Patent Application WO/2022/205578
Kind Code:
A1
Abstract:
A high-throughput automated gene synthesis apparatus based on a cluster array. The high-throughput gene synthesis apparatus comprises a substrate and a microwell plate, wherein the substrate is provided with several clusters of microwells (1); a sidewall surface of the microwell (1) is chemically modified and is then used as a solid-phase carrier for nucleic acid synthesis, or the microwell (1) is filled with solid-phase carriers; and the several clusters of microwells (1) are arranged in a cluster array, each cluster of microwells (1) has the same size as that of each hole (2) on the microwell plate, and the position of each cluster of microwells (1) corresponds to the position of each hole (2). When oligonucleotides are synthesized using the apparatus, the synthesized oligonucleotides are automatically recovered into a standard SBS plate (a 96-well plate, a 384-well plate, a 1536-well plate, etc.) having a corresponding size under the apparatus, thereby forming oligonucleotide pools required for each gene. The yield of the oligonucleotides is at a pmoL level, and is sufficient for direct subsequent gene assembly by means of polymerase cycling assembly (PCA) or ligase chain reaction (LCR) without amplification. The oligonucleotides which have been subjected to error correction can thus be used for full-length gene splicing, thereby realizing high-throughput automated gene synthesis.
Inventors:
ZHANG MANCANG (CN)
HOU CAILING (CN)
YU DONGPING (CN)
HOU CAILING (CN)
YU DONGPING (CN)
Application Number:
PCT/CN2021/094310
Publication Date:
October 06, 2022
Filing Date:
May 18, 2021
Export Citation:
Assignee:
DYNEGE BIOSCIENCE CORP (CN)
International Classes:
B01J19/00
Foreign References:
| CN102962015A | 2013-03-13 | |||
| US20080084008A1 | 2008-04-10 | |||
| CN212237207U | 2020-12-29 | |||
| CN102272363A | 2011-12-07 | |||
| CN107488580A | 2017-12-19 | |||
| CN1414006A | 2003-04-30 | |||
| JPH07132077A | 1995-05-23 | |||
| CN109603704A | 2019-04-12 |
Other References:
LI HUI, HUANG YE, WEI ZEWEN, WANG WEI, YANG ZHENJUN, LIANG ZICAI, LI ZHIHONG: "An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer", SCIENTIFIC REPORTS, vol. 9, no. 1, 1 December 2019 (2019-12-01), XP055971942, DOI: 10.1038/s41598-019-41519-0
CHENG ET AL.: "High throughput parallel synthesis of oligonucleotides with 1536 channel syntheizer", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 30, no. 18, 15 September 2002 (2002-09-15), GB , pages e93 - e93-7, XP008139574, ISSN: 0305-1048, DOI: 10.1093/nar/gnf092
CHENG ET AL.: "High throughput parallel synthesis of oligonucleotides with 1536 channel syntheizer", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 30, no. 18, 15 September 2002 (2002-09-15), GB , pages e93 - e93-7, XP008139574, ISSN: 0305-1048, DOI: 10.1093/nar/gnf092
Attorney, Agent or Firm:
JEEKAI&PARTNERS (CN)
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