HIGH-THROUGHPUT BODY FLUID PROTEIN SAMPLE PRETREATMENT APPARATUS AND APPLICATION THEREOF

WIPO Patent Application WO/2018/090652

A1

A high-throughput body fluid protein sample pretreatment apparatus. The apparatus is a system integrating high-abundance protein removal from body fluids (plasma, serum, and urine), and medium- and low-abundance protein online denaturation and reduction, desalting, and on-line enzymolysis, and comprises: a high-abundance body fluid protein removal antibody column (3), a cluster type high temperature denaturation and reduction device, a cluster-type solvent displacer, and an immobilized enzyme reactor (12). High-abundance proteins are first removed from a body fluid protein sample by means of the antibody column (3); then the eluted medium- and low-abundance proteins are then subjected to online and rapid denaturation and reduction by the cluster-type high-temperature denaturation and reduction device; the denaturing agent and the reducing agent are removed by means of the cluster-type solvent displacer; finally, online enzymolysiss is conducted by the immobilized enzyme reactor (12), and peptides generated by means of enzymolysiss can be tested by means of mass spectrometry. The high-throughput body fluid protein sample pretreatment apparatus integrates complex sample pretreatment processes for body fluid proteins.

ZHANG LIHUA (CN)
YUAN HUIMING (CN)
DAI ZHONGPENG (CN)
YANG KAIGUANG (CN)
ZHANG YUKUI (CN)

PCT/CN2017/092561

May 24, 2018

July 12, 2017

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DALIAN INST CHEM & PHYSICS CAS (CN)

G01N30/06; G01N30/02

CN103852527A	2014-06-11
CN103212217A	2013-07-24
CN104713963A	2015-06-17
CN103864967A	2014-06-18
CN104634915A	2015-05-20
US20110118453A1	2011-05-19
US20060154302A1	2006-07-13

SHENYANG PATENT & TRADEMARK AGENCY ACADEMIA SINICA (CN)

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