Title:
HIGH-THROUGHPUT MULTIPARAMETRIC SINGLE CELL-DERIVED EXTRACELLULAR VESICLE ANALYSIS CHIP AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/218101
Kind Code:
A1
Abstract:
Provided are a high-throughput multiparametric single cell-derived extracellular vesicle analysis chip and use thereof. The chip comprises a two-layer structure, one layer being a bioanalytical substrate for extracellular vesicle capture and detection, the other layer being a chip with a high throughput array for single cell capture. The microfluidic chip technology and the single cell analysis technology are applied to the study of extracellular vesicles, and high-throughput and multiparametric capture can be achieved by using an immunoaffinity method, thereby detecting extracellular vesicles of various sizes secreted by a single cell. Further, qualitative and quantitative detections, correlation analysis, multifunctional analysis, and immunophenotyping analysis of the secretion of extracellular vesicles at a single cell level are carried out, clearly demonstrating the heterogeneity of cells and the secreted extracellular vesicles among the cells.
Inventors:
LU YAO (CN)
JI YAHUI (CN)
LI LINMEI (CN)
LIN BINGCHENG (CN)
JI YAHUI (CN)
LI LINMEI (CN)
LIN BINGCHENG (CN)
Application Number:
PCT/CN2018/000219
Publication Date:
November 21, 2019
Filing Date:
June 11, 2018
Export Citation:
Assignee:
DALIAN INST CHEM & PHYSICS CAS (CN)
International Classes:
C12M3/00; C12M3/08
Foreign References:
| CN104884605A | 2015-09-02 | |||
| CN101748059A | 2010-06-23 | |||
| CN101168717A | 2008-04-30 | |||
| CN206787889U | 2017-12-22 | |||
| CN104760922A | 2015-07-08 |
Attorney, Agent or Firm:
SHENYANG CHENCHUANG TECHNICAL PATENT AGENT LTD (CN)
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