Title:
HIGH-THROUGHPUT QUANTITATIVE DETERMINATION METHOD OF FREE OLIGOSACCHARIDES IN MILK
Document Type and Number:
WIPO Patent Application WO/2020/252677
Kind Code:
A1
Abstract:
A high-throughput quantitative determination method of free oligosaccharides in milk, comprising the following steps: sample preparation and detection of the prepared samples using ultra-high performance liquid chromatography; the sample preparation comprises the following steps: diluting a milk sample with ultrapure water, centrifuging to remove milk fat and/or milk protein to obtain free oligosaccharides, implementing fluorescence labelling of the free oligosaccharides and, before machine detection, using ultrapure water to dilute the concentration of fluorescence labelled oligosaccharides to within the range of a standard curve.
Inventors:
CHEN LIJUN (CN)
HUANG XUNWEN (CN)
YANG CHUNYING (CN)
JIANG TIEMIN (CN)
QIAO WEICANG (CN)
LIU YANPIN (CN)
ZHOU WEIMING (CN)
HUANG XUNWEN (CN)
YANG CHUNYING (CN)
JIANG TIEMIN (CN)
QIAO WEICANG (CN)
LIU YANPIN (CN)
ZHOU WEIMING (CN)
Application Number:
PCT/CN2019/091827
Publication Date:
December 24, 2020
Filing Date:
June 19, 2019
Export Citation:
Assignee:
BEIJING SANYUAN FOODS CO LTD (CN)
International Classes:
G01N30/02; G01N21/64; G01N33/04
Foreign References:
| CN107192771A | 2017-09-22 | |||
| CN101107526A | 2008-01-16 | |||
| CN109030658A | 2018-12-18 | |||
| CN103852531A | 2014-06-11 | |||
| CN104749290A | 2015-07-01 | |||
| CN104678042A | 2015-06-03 | |||
| JPH0432763A | 1992-02-04 |
Other References:
ZHU, JING ET AL.: "Detection and Comparison of 10 Human Milk Oligosaccharides During Lactational Stage", CHINESE JOURNAL OF FOOD HYGIENE, vol. 29, no. 4, 31 August 2017 (2017-08-31), ISSN: 1004-8456, DOI: 20190827160623X
Attorney, Agent or Firm:
BEIJING YUEHE INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
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