HIGH-YIELD L-HISTIDINE GENETICALLY ENGINEERED BACTERIUM STRAIN, AND CONSTRUCTION METHOD THEREFOR AND APPLICATION THEREOF

Title:

HIGH-YIELD L-HISTIDINE GENETICALLY ENGINEERED BACTERIUM STRAIN, AND CONSTRUCTION METHOD THEREFOR AND APPLICATION THEREOF

Document Type and Number:

WIPO Patent Application WO/2020/237701

Kind Code:

A1

Abstract:

Provided are a high-yield L-histidine genetically engineered bacterium strain, and a construction method therefor and an application thereof, integrating a corynebacterium glutamicum ATP phosphoribosyl transferase HisG mutant encoding gene into a genome of escherichia coli to enhance the activity of HisG, also increasing the number of copies of histidine operon genes in the genome to enhance synthesis of histidine, and integrating an encoding gene lysE of arginine/lysine transport protein derived from the corynebacterium glutamicum into the genome to promote extracellular secretion of intracellular histidine. The genetically engineered bacterium is used for producing the L-histidine by a fermentation method, can stably produce 40-55 g/L of the histidine by fermentation in a 5 L fermentation tank for 40-50 h, has a production intensity reaching 1.0-1.5 g/(Lxh), and has a conversion rate of 0.18-0.22 g histidine/g glucose.

Inventors:

XIE XIXIAN (CN)
ZHANG YUE (CN)
FAN WEIMING (CN)
JIANG WEI (CN)
WU HEYUN (CN)
CHEN YANNA (CN)
TIAN DAOGUANG (CN)
TU JIANQING (CN)

Application Number:

PCT/CN2019/089938

Publication Date:

December 03, 2020

Filing Date:

June 04, 2019

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Assignee:

TIANJIN UNIV OF SCIENCE&TECHNOLOGY (CN)
ZHEJIANG ZHENYUAN PHARMACEUTICAL CO LTD (CN)

International Classes:

C12N1/21; C12P13/24; C12R1/19

Foreign References:

TW201910512A	2019-03-16
CN104845923A	2015-08-19
US20060008546A1	2006-01-12
CN1918283A	2007-02-21
CN102453691A	2012-05-16
CN103146593A	2013-06-12

Other References:

KULIS-HORN RK ET AL.: "Non-official translation: Production of L-histidine on the Basis of Feedback Inhibition Strategy of Eliminating Corynebacterium Glutamicum Adenosine Triphosphate Phosphate Ribosyltransferase", CHINESE JOURNAL OF PHARMACEUTICALS, vol. 46, no. 8, 31 August 2015 (2015-08-31), ISSN: 1001-8255, DOI: 20200224114416A
GENBANK: "accession number WP_074495858: ATP Phosphoribosyltransferase [Corynebacterium Glutamicum]", CHINESE JOURNAL OF PHARMACEUTICALS, 22 December 2016 (2016-12-22), DOI: 20200224114552A

Attorney, Agent or Firm:

BEIJING RISINGMARK INTELLECTUAL PROPERTY AGENCY (CN)

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