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Title:
HIGHLY ACTIVE S-CYANOHYDRIN LYASE AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/127143
Kind Code:
A1
Abstract:
The invention provides a highly active S-cyanohydrin lyase obtained by mutating an amino acid residue at position 103 of a wild-type cassava S-cyanohydrin lyase. The mutation can significantly increase an expression of a mutant enzyme in E. coli and does not require a decrease in temperature when induced. Further mutations at position 128 and other sites were performed to obtain mutants with increased catalytic activity.

Inventors:
TIAN ZHENHUA (CN)
QU XUDONG (CN)
CHENG ZHANBING (CN)
SUN CHUANMIN (CN)
Application Number:
PCT/CN2018/071619
Publication Date:
July 12, 2018
Filing Date:
January 05, 2018
Export Citation:
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Assignee:
ABIOCHEM BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N9/88; C12N15/60; C12N15/70; C12P13/00
Domestic Patent References:
WO2005095602A12005-10-13
Foreign References:
CN106032531A2016-10-19
CN106032543A2016-10-19
CN102899306A2013-01-30
Other References:
DATABASE Protein [O] 14 April 2016 (2016-04-14), "Chain A, Crystal Structure of S-hydro-xynitrile Lyase from Manihot Esculenta (his103leu)", XP055513833, Database accession no. 4YK7_A
PLANT SCI., vol. 108, 1995, pages 1
PLANT PHYSIOL, vol. 116, 1998, pages 1219
SELMAR, ANALYTICAL BIOCHEMISTRY, vol. 166, 1987, pages 208 - 211
SAMBROOK. J ET AL.: "Molecular Cloning-A Laboratory Manual", 2002, SCIENCE PRESS
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
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