Title:
A HIGHLY ERROR-PRONE ORTHOGONAL DNA REPLICATION SYSTEM FOR TARGETED CONTINUOUS EVOLUTION IN VIVO
Document Type and Number:
WIPO Patent Application WO/2019/079775
Kind Code:
A3
Abstract:
The invention provides compositions comprising highly error-prone polymerases and methods of using the polymerases for rapid evolution of a nucleic acid sequence within host cells. The invention further provides a versatile synthetic biology platform for manipulating DNA replication inside a cell. The invention also provides a mutually orthogonal replication system for manipulating and tuning DNA replication of multiple nucleic acid molecules using the error-prone polymerases.
Inventors:
LIU CHANG (US)
RAVIKUMAR ARJUN (US)
ARZUMANYAN GARRI (US)
JAVANPOUR ALEX (US)
RAVIKUMAR ARJUN (US)
ARZUMANYAN GARRI (US)
JAVANPOUR ALEX (US)
Application Number:
PCT/US2018/056794
Publication Date:
June 06, 2019
Filing Date:
October 19, 2018
Export Citation:
Assignee:
UNIV CALIFORNIA (US)
International Classes:
C12N15/81; C12N1/18; C12N15/54
Other References:
RAVIKUMAR ET AL.: "An orthogonal DNA replication system in yeast", NAT CHEM BIOL, vol. 10, no. 3, March 2014 (2014-03-01), pages 174 - 177, XP055618678
HERR ET AL.: "Mutator suppression and escape from replication error-induced extinction in yeast", PLOS GENET, vol. 7, no. 10, October 2011 (2011-10-01), pages e1002282, XP055618680
DATABASE Nucleotide [online] 25 July 2016 (2016-07-25), "Yeast DNA killer plasmid pGKL1", XP055618682, retrieved from ncbi Database accession no. X01095.1
HERR ET AL.: "Mutator suppression and escape from replication error-induced extinction in yeast", PLOS GENET, vol. 7, no. 10, October 2011 (2011-10-01), pages e1002282, XP055618680
DATABASE Nucleotide [online] 25 July 2016 (2016-07-25), "Yeast DNA killer plasmid pGKL1", XP055618682, retrieved from ncbi Database accession no. X01095.1
Attorney, Agent or Firm:
HARRIMAN, JD (US)
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