Title:
HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS PROTEIN VACCINE DERIVED FROM TRANSGENIC PLANTS, AND METHOD FOR PREPARING SAME
Document Type and Number:
WIPO Patent Application WO/2011/043584
Kind Code:
A2
Abstract:
The present invention relates to a method for producing transgenic plants, which involves producing hemagglutinin proteins of the H5N1 virus in a highly efficient manner using a plant transformation recombinant vector, wherein said vector can express, in a highly efficient manner, hemagglutinin proteins of the H5N1 virus which is the highly pathogenic avian influenza virus, and can transport proteins expressed in plants to the endoplasmic reticulum and enable the retention of the proteins in the endoplasmic reticulum so as to accomplish glycosylation which is necessarily required for antigen activity. The present invention also relates to a method for the mass production of hemagglutinin proteins of the antigenic H5N1 virus from the transgenic plants, and to a vaccine composition for the avian influenza virus comprising the transgenic plants or the hemagglutinin proteins produced from the plants. The method for producing hemagglutinin proteins of the H5N1 virus using transgenic plants according to the present invention enables the mass production of hemagglutinin proteins at a low cost. The plant transformation recombinant vector used in the present invention has a cellulose-binding domain inserted therein to separate proteins expressed in plants in a quick and easy manner. In addition, transgenic plants according to the present invention can be taken in the form of feed additives or the like, and therefore can be used as edible vaccines. Further, antigenic hemagglutinin proteins produced by the method induce immune response when administered to an animal model, and therefore can be used not only as protein vaccines for the H5N1 avian influenza virus, but also as diagnostic reagents for avian influenza virus infection.
More Like This:
Inventors:
HWANG IN HWAN (KR)
SOHN EUN JU (KR)
KWON EUN HYE (KR)
NA YUN JEONG (KR)
JEON EUN HYUN (KR)
IM SE JIN (KR)
PARK KI SEOK (KR)
SUNG YOUNG CHUL (KR)
SOHN EUN JU (KR)
KWON EUN HYE (KR)
NA YUN JEONG (KR)
JEON EUN HYUN (KR)
IM SE JIN (KR)
PARK KI SEOK (KR)
SUNG YOUNG CHUL (KR)
Application Number:
PCT/KR2010/006821
Publication Date:
April 14, 2011
Filing Date:
October 06, 2010
Export Citation:
Assignee:
HELIX CO LTD (KR)
HWANG IN HWAN (KR)
SOHN EUN JU (KR)
KWON EUN HYE (KR)
NA YUN JEONG (KR)
JEON EUN HYUN (KR)
IM SE JIN (KR)
PARK KI SEOK (KR)
SUNG YOUNG CHUL (KR)
HWANG IN HWAN (KR)
SOHN EUN JU (KR)
KWON EUN HYE (KR)
NA YUN JEONG (KR)
JEON EUN HYUN (KR)
IM SE JIN (KR)
PARK KI SEOK (KR)
SUNG YOUNG CHUL (KR)
International Classes:
A01H1/00; A61K39/12; C12N15/40; C12N15/82
Foreign References:
| KR20090081403A | 2009-07-28 |
Other References:
GOFFARD, A. ET AL., J. VIROL., vol. 79, 2005, pages 8400 - 8409
HEBERT, D. N. ET AL., J. CELL BIOL., vol. 139, 1997, pages 613 - 623
EWASYSHYN, M. ET AL., J. GEN. VIROL., vol. 74, 1993, pages 2781 - 2785
MANIATIS ET AL.: "Molecular Cloning: A Laboratory Manual", 1982, COLD SPRING HARBOR LABORATORY
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
DEUTSCHER, M.: "Guide to Protein Purification Methods Enzymology", vol. 182, 1990, ACADEMIC PRESS. INC.
See also references of EP 2486791A4
HEBERT, D. N. ET AL., J. CELL BIOL., vol. 139, 1997, pages 613 - 623
EWASYSHYN, M. ET AL., J. GEN. VIROL., vol. 74, 1993, pages 2781 - 2785
MANIATIS ET AL.: "Molecular Cloning: A Laboratory Manual", 1982, COLD SPRING HARBOR LABORATORY
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
DEUTSCHER, M.: "Guide to Protein Purification Methods Enzymology", vol. 182, 1990, ACADEMIC PRESS. INC.
See also references of EP 2486791A4
Attorney, Agent or Firm:
WIE, Byoung-Gab et al. (KR)
위병갑 (KR)
위병갑 (KR)
Download PDF:
Previous Patent: TREATMENT SYSTEM USING A MULTICHANNEL SINE WAVE GENERATING APPARATUS
Next Patent: BOOKMARK
Next Patent: BOOKMARK