HIGHLY SENSITIVE DETERMINATION OF D-3-HYDROXYBUTYRIC ACID OR ACETOACETIC ACID AND COMPOSITION THEREFOR

Title:

HIGHLY SENSITIVE DETERMINATION OF D-3-HYDROXYBUTYRIC ACID OR ACETOACETIC ACID AND COMPOSITION THEREFOR

Document Type and Number:

WIPO Patent Application WO/1993/012254

Kind Code:

A1

Abstract:

Determination of D-3-hydroxybutyric acid or acetoacetic acid by reacting a specimen solution with a reagent containing (1) D-3-hydroxybutyric acid dehydrogenase, (2) A1, and (3) B1 to form the cycle (I) and determining the amount of A2 formed or the amount of B1 consumed; and a composition containing the ingredients (1), (2), and (3) for use in the above determination method. The method and the composition make it possible to determine a small amount of a specimen solution conveniently with high sensitivity and are useful in the field of clinical inspection and food testing.

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Inventors:

UEDA SHIGERU (JP)
MISAKI HIDEO (JP)
IKUTA SHIGERU (JP)
TAKAHASHI MAMORU (JP)

Application Number:

PCT/JP1991/001706

Publication Date:

June 24, 1993

Filing Date:

December 12, 1991

Export Citation:

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Assignee:

ASAHI CHEMICAL IND (JP)

International Classes:

C12Q1/32; (IPC1-7): C12Q1/32

Other References:

SHADAN HOJIN NIHON SEIKAGAKU-KAI (author), "Biochemical Experiment Lecture 5 Enzyme Research (upper)", August 20, 1975 (20.08.75), Tokyo Kagaku Dojin, p. 121-135.

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