HIGHLY SENSITIVE METHOD OF DETERMINING THE QUANTITY OF HERBAL MEDICINE-DERIVED COMPONENTS

WIPO Patent Application WO/2012/067090

A1

The disclosed highly sensitive method of determining the quantity of herbal medicine-derived components involves: an extraction step in which, after injecting into a solid phase with reversed-phase distribution capability and anion exchange capability a mixture comprising a biological sample mixed with an alkali or an alcohol, the aforementioned solid phase is washed at least once with a mixed solution comprising one or more substances selected from a group consisting of water, alkalines, alcohols, and acetonitrile, and thereafter, an extract is prepared containing a herbal medicine-derived component by means of elution from the aforementioned solid phase by means of acidic alcohol; and a step for using mass spectrometry to detect and measure, in the extract extracted in the aforementioned extraction step, at least one substance of a group consisting of glycyrrhizin, glycyrrhetinic acid, glycyrrhizin and glycyrrhetinic acid metabolites, glycyrrhizin and glycyrrhetinic acid analogs, the saponin component contained in licorice, and pharmaceutically acceptable salts of these.

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SUZUKI KAYOKO (JP)
SUZUKI TSUNEHARU (JP)
TSUKAHARA MICHIKO (JP)
HARUTA YUKO (JP)
HATTA AKIRA (JP)
HAMADA YUJI (JP)
INOUE HIDEO (JP)

PCT/JP2011/076248

May 24, 2012

November 15, 2011

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MINOPHAGEN PHARMACEUTICAL CO LTD (JP)
SUZUKI KAYOKO (JP)
SUZUKI TSUNEHARU (JP)
TSUKAHARA MICHIKO (JP)
HARUTA YUKO (JP)
HATTA AKIRA (JP)
HAMADA YUJI (JP)
INOUE HIDEO (JP)

G01N30/88; G01N27/62; G01N30/06; G01N30/72

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HIROKO MATSUMOTO ET AL.: "Determination of cyclamate and dulcin by HPLC and LC/MS/MS and systematic analysis of eight types of sweeteners", KAGAKU SEIBUTSU SOGO KANRI, vol. 6, no. 1, March 2010 (2010-03-01), pages 25 - 35

SHIGA Masatake et al. (JP)
Masatake Shiga (JP)

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