Title:
HORSESHOE CRAB FACTOR B VARIANT
Document Type and Number:
WIPO Patent Application WO/2018/159771
Kind Code:
A1
Abstract:
The present invention provides a technique relating to a horseshoe crab factor B variant and, in its turn, provides a means for highly sensitively assaying an endotoxin. A polypeptide having an amino acid sequence, in which the amino acid residue at the 193-position in the amino acid sequence of horseshoe crab factor B polypeptide is substituted into a cysteine (Cys) residue, is produced. By preparing a Limulus reagent using a combination of the thus obtained polypeptide with horseshoe crab factor C, an endotoxin can be assayed at a high sensitivity.
Inventors:
KOBAYASHI YUKI (JP)
MIZUMURA HIKARU (JP)
ODA TOSHIO (JP)
KAWABATA SHUN-ICHIRO (JP)
MIZUMURA HIKARU (JP)
ODA TOSHIO (JP)
KAWABATA SHUN-ICHIRO (JP)
Application Number:
PCT/JP2018/007840
Publication Date:
September 07, 2018
Filing Date:
March 01, 2018
Export Citation:
Assignee:
SEIKAGAKU KOGYO CO LTD (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/50; C12P21/02; C12Q1/37; G01N33/579
Domestic Patent References:
| WO2012118226A1 | 2012-09-07 | |||
| WO2014092079A1 | 2014-06-19 |
Other References:
MUTA, TATSUSHI ET AL.: "Horseshoe Crab Coagulation Factor B", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 28, 5 October 1993 (1993-10-05), pages 21384 - 21388, XP055543462, Retrieved from the Internet
TAKAGI, HIROSHI: "Functional Improvement of Subtilisin E, a Protease of Bacillus subtilis, by Protein Engineering", NIPPON NOGEIKAGAKU KAISHI, vol. 71, no. 10, 1997, pages 995 - 1002, XP055543469, Retrieved from the Internet
OGINO, HIROYASU ET AL.: "Role of Intermolecular Disulfide Bonds of the Organic Solvent-Stable PST-01 Protease in Its Organic Sol vent Stability", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 67, no. 2, February 2001 (2001-02-01), pages 942 - 947, XP055543474, Retrieved from the Internet
VARALLYAY, EVA ET AL.: "The Role of Disulfide Bond C191-C220 in Trypsin and Chymotrypsin", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 230, 1997, pages 592 - 596, XP055543480, Retrieved from the Internet
NAKAMURA, T.HORIUCHI, T.MORITA T.IWANAGA, S., J. BIOCHEM., vol. 99, 1986, pages 847 - 57
MUTA, T.ODA, T.IWANAGA, S., J. BIOL. CHEM., vol. 268, 1993, pages 21384 - 8
KARLIN, S.ALTSCHUL, SF., PROC. NATL. ACAD. SCI. U.S.A., vol. 90, 1993, pages 5873 - 7
PEARSON, WR., METHODS. ENZYMOL., vol. 183, 1990, pages 63 - 98
"NCBI", Database accession no. XP_013783518.1
SAMBROOK J ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS, pages: 61 - 70
KUNKEL, T.A.ROBERTS, J.D.ZAKOUR, R.A., METHODS ENZYMOL., vol. 154, 1987, pages 367 - 403
TAKEDA, M.OHNO, S.SEKI, F.NAKATSU, Y.TAHARA, M.YANAGI, Y., J. VIROL., vol. 79, 2005, pages 14346 - 54
MIYATA ET AL., TANPAKUSHITSU KAKUSAN KOSO BESSATSU (PROTEINS, NUCLEIC ACIDS, AND ENZYMES: EXTRA ISSUE, 1986, pages 30 - 43
KOBAYASHI, Y.TAKAHASHI, T.SHIBATA, T.IKEDA, S.KOSHIBA, T.MIZUMURA, H.ODA, T.KAWABATA, S., J. BIOL. CHEM., vol. 290, 2015, pages 19379 - 86
KOSHIBA, T.HASHII, T.KAWABATA, S., J. BIOL. CHEM., vol. 282, 2007, pages 3962 - 7
ARICESCU, AR.LU, W.JONES, EY., ACTA CRYSTALLOGR. D BIOL. CRYSTALLOGR., vol. 62, 2006, pages 1243 - 50
See also references of EP 3591049A4
TAKAGI, HIROSHI: "Functional Improvement of Subtilisin E, a Protease of Bacillus subtilis, by Protein Engineering", NIPPON NOGEIKAGAKU KAISHI, vol. 71, no. 10, 1997, pages 995 - 1002, XP055543469, Retrieved from the Internet
OGINO, HIROYASU ET AL.: "Role of Intermolecular Disulfide Bonds of the Organic Solvent-Stable PST-01 Protease in Its Organic Sol vent Stability", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 67, no. 2, February 2001 (2001-02-01), pages 942 - 947, XP055543474, Retrieved from the Internet
VARALLYAY, EVA ET AL.: "The Role of Disulfide Bond C191-C220 in Trypsin and Chymotrypsin", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 230, 1997, pages 592 - 596, XP055543480, Retrieved from the Internet
NAKAMURA, T.HORIUCHI, T.MORITA T.IWANAGA, S., J. BIOCHEM., vol. 99, 1986, pages 847 - 57
MUTA, T.ODA, T.IWANAGA, S., J. BIOL. CHEM., vol. 268, 1993, pages 21384 - 8
KARLIN, S.ALTSCHUL, SF., PROC. NATL. ACAD. SCI. U.S.A., vol. 90, 1993, pages 5873 - 7
PEARSON, WR., METHODS. ENZYMOL., vol. 183, 1990, pages 63 - 98
"NCBI", Database accession no. XP_013783518.1
SAMBROOK J ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS, pages: 61 - 70
KUNKEL, T.A.ROBERTS, J.D.ZAKOUR, R.A., METHODS ENZYMOL., vol. 154, 1987, pages 367 - 403
TAKEDA, M.OHNO, S.SEKI, F.NAKATSU, Y.TAHARA, M.YANAGI, Y., J. VIROL., vol. 79, 2005, pages 14346 - 54
MIYATA ET AL., TANPAKUSHITSU KAKUSAN KOSO BESSATSU (PROTEINS, NUCLEIC ACIDS, AND ENZYMES: EXTRA ISSUE, 1986, pages 30 - 43
KOBAYASHI, Y.TAKAHASHI, T.SHIBATA, T.IKEDA, S.KOSHIBA, T.MIZUMURA, H.ODA, T.KAWABATA, S., J. BIOL. CHEM., vol. 290, 2015, pages 19379 - 86
KOSHIBA, T.HASHII, T.KAWABATA, S., J. BIOL. CHEM., vol. 282, 2007, pages 3962 - 7
ARICESCU, AR.LU, W.JONES, EY., ACTA CRYSTALLOGR. D BIOL. CRYSTALLOGR., vol. 62, 2006, pages 1243 - 50
See also references of EP 3591049A4
Attorney, Agent or Firm:
SANUKI, Shinichi et al. (JP)
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