Title:
HRM DETECTION PRIMER, KIT, AND METHOD FOR QUICKLY IDENTIFYING CLASSICAL STRAIN AND VARIANT STRAIN OF PORCINE EPIDEMIC DIARRHEA VIRUS
Document Type and Number:
WIPO Patent Application WO/2018/059195
Kind Code:
A1
Abstract:
A HRM detection primer, kit, and method for quickly distinguishing a classical strain from a variant strain of porcine epidemic diarrhea virus. According to the method, by taking virus nucleic acid extracted from a sample as a template, RT-PCR amplification is performed on the primer and a fluorescence saturated dye to obtain an amplification product, and HRM analysis is performed on the amplification product to determine a virus type.
Inventors:
PAN YU (CN)
Application Number:
PCT/CN2017/100435
Publication Date:
April 05, 2018
Filing Date:
September 05, 2017
Export Citation:
Assignee:
GUANGZHOU VETERINARY BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12Q1/70; C12N15/11; C12Q1/68
Domestic Patent References:
| WO2011050050A2 | 2011-04-28 |
Foreign References:
| CN106319092A | 2017-01-11 | |||
| CN104531902A | 2015-04-22 | |||
| CN102858962A | 2013-01-02 | |||
| CN105018645A | 2015-11-04 | |||
| CN105154584A | 2015-12-16 | |||
| CN103320535A | 2013-09-25 |
Other References:
QIN, YIBIN ET AL.: "Development and applications of an RT-PCR method for differentiation between variant porcine epidemic diarrhea virus strains and classical strains", CHINESE VETERINARY SCIENCE, vol. 44, no. 05, 31 December 2014 (2014-12-31), pages 509 - 514
NING, P. ET AL.: "Detection and differentiation of classical swine fever virus strains C and Shimen by high-resolution melt analysis", JOURNAL OF VIROLOGICAL METHODS, vol. 194, 27 August 2013 (2013-08-27), pages 129 - 131, XP055500442
TITOV, I. ET AL.: "Genotyping of classical swine fever virus using high-resolution melt analysis", JOURNAL OF VIROLOGICAL METHODS, vol. 224, 20 August 2015 (2015-08-20), pages 53 - 57, XP055500443
HE, JIANG ET AL.: "Application of high resolution melting curve analysis technology on the species identification and classification", CHINESE JOURNAL OF VECTOR BIOLOGY AND CONTROL, vol. 27, no. 2, 30 April 2016 (2016-04-30), pages 206 - 208, 210
NING, P. ET AL.: "Detection and differentiation of classical swine fever virus strains C and Shimen by high-resolution melt analysis", JOURNAL OF VIROLOGICAL METHODS, vol. 194, 27 August 2013 (2013-08-27), pages 129 - 131, XP055500442
TITOV, I. ET AL.: "Genotyping of classical swine fever virus using high-resolution melt analysis", JOURNAL OF VIROLOGICAL METHODS, vol. 224, 20 August 2015 (2015-08-20), pages 53 - 57, XP055500443
HE, JIANG ET AL.: "Application of high resolution melting curve analysis technology on the species identification and classification", CHINESE JOURNAL OF VECTOR BIOLOGY AND CONTROL, vol. 27, no. 2, 30 April 2016 (2016-04-30), pages 206 - 208, 210
Attorney, Agent or Firm:
JIAQUAN IP LAW FIRM (CN)
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