Title:
HUMAN BLOOD-DERIVED BLOOD CELL MASS ASSAY USING CELL COAGULATION, BLOOD ADULT STEM CELLS USING SAME, METHOD FOR EXPANDING PROGENITOR CELLS, AND STEM CELLS PRODUCED USING THE METHOD
Document Type and Number:
WIPO Patent Application WO/2012/026687
Kind Code:
A3
Abstract:
The present invention relates to a technique of using monocytic blood cells to effectively culture and proliferate blood adult stem cells and progenitor cells that only exist in small quantities to effectively obtain large quantities of stem cells. According to the present invention, the limitation of being able to derive only small quantities of stem cells from blood can be overcome, and the pluripotency of stem cells can easily be obtained.
Inventors:
PARK YOUNG-BAE (KR)
KIM HYO-SOO (KR)
KANG HYUN-JAE (KR)
CHO HYUN-JAI (KR)
HUR JIN (KR)
PARK JONG-HAN (KR)
JANG JAE-HEE (KR)
KIM HYO-SOO (KR)
KANG HYUN-JAE (KR)
CHO HYUN-JAI (KR)
HUR JIN (KR)
PARK JONG-HAN (KR)
JANG JAE-HEE (KR)
Application Number:
PCT/KR2011/005762
Publication Date:
April 19, 2012
Filing Date:
August 08, 2011
Export Citation:
Assignee:
UNIV SEOUL NAT R & DB FOUND (KR)
PARK YOUNG-BAE (KR)
KIM HYO-SOO (KR)
KANG HYUN-JAE (KR)
CHO HYUN-JAI (KR)
HUR JIN (KR)
PARK JONG-HAN (KR)
JANG JAE-HEE (KR)
PARK YOUNG-BAE (KR)
KIM HYO-SOO (KR)
KANG HYUN-JAE (KR)
CHO HYUN-JAI (KR)
HUR JIN (KR)
PARK JONG-HAN (KR)
JANG JAE-HEE (KR)
International Classes:
C12N5/0789; C12N5/02; C12N5/0786
Other References:
CASE ET AL.: "Human CD34+ AC133+ VEGFR-2+ cells are not endothelial progenitor cells but distinct, primitive hematopoietic progenitors", EXPERIMENTAL HEMATOLOGY, vol. 35, 2007, pages 1109 - 1118, XP022121924
SUURONEN ET AL.: "Generation of CD133+ cells from CD133- peripheral blood mononuclear cells and their properties", CARDIOVASCULAR RESEARCH, vol. 70, 10 March 2006 (2006-03-10), pages 126 - 135, XP025011436
PEICHEV ET AL.: "Expression of VEGFR-2 and AC1333 by circulating human CD34+ cells identifies a population of functional endothelial precursors", BLOOD, vol. 95, no. 3, 2000, pages 952 - 958, XP002251073
KWON ET AL.: "Suspension culture of hematopoietic stem cells in stirred bioreactors", BIOTECHNOLOGY LETTERS, vol. 25, 2003, pages 179 - 182, XP055029864
OGAWA ET AL.: "Suspension culture of human mast cells/basophils from umbilical cord blood mononuclear cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 80, July 1983 (1983-07-01), pages 4494 - 4498, XP055095735
HUR ET AL.: "Human peripheral blood-born hematosphere as a niche of hematopoietic stem cell expansion", CELL RESEARCH, vol. 21, 12 April 2011 (2011-04-12), pages 987 - 990, XP055095736
SUURONEN ET AL.: "Generation of CD133+ cells from CD133- peripheral blood mononuclear cells and their properties", CARDIOVASCULAR RESEARCH, vol. 70, 10 March 2006 (2006-03-10), pages 126 - 135, XP025011436
PEICHEV ET AL.: "Expression of VEGFR-2 and AC1333 by circulating human CD34+ cells identifies a population of functional endothelial precursors", BLOOD, vol. 95, no. 3, 2000, pages 952 - 958, XP002251073
KWON ET AL.: "Suspension culture of hematopoietic stem cells in stirred bioreactors", BIOTECHNOLOGY LETTERS, vol. 25, 2003, pages 179 - 182, XP055029864
OGAWA ET AL.: "Suspension culture of human mast cells/basophils from umbilical cord blood mononuclear cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 80, July 1983 (1983-07-01), pages 4494 - 4498, XP055095735
HUR ET AL.: "Human peripheral blood-born hematosphere as a niche of hematopoietic stem cell expansion", CELL RESEARCH, vol. 21, 12 April 2011 (2011-04-12), pages 987 - 990, XP055095736
Attorney, Agent or Firm:
ERUUM PATENT LAW FIRM (1005-8Daechi-dong, Kangnam-gu, Seoul 135-851, KR)
Download PDF:
Claims: