Title:
HUMAN FIBROBLAST GROWTH FACTOR-2 MUTANT WITH INCREASED STABILITY, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/195157
Kind Code:
A1
Abstract:
The present invention relates to a highly stable basic fibroblast growth factor mutant, and a use thereof. More specifically, the present invention provides: a highly stable basic fibroblast growth factor (bFGF) mutant, in which two or more amino acids in an amino acid sequence of SEQ ID NO: 1 are substituted with serine and one or more amino acids are substituted with cysteine; a DNA base sequence encoding the bFGF mutant; an expression vector comprising the DNA base sequence; a transformant transformed by the expression vector; a method of producing the bFGF mutant; and a composition comprising the bFGF mutant as an active ingredient. According to the present invention, the bFGF mutant of the present invention has excellent stability in an aqueous solution state and excellent thermal stability, and thus it is possible to produce functional cosmetics and skin inflammation medicines which do not lose activity, unlike conventional wild-type bFGF products, even during distribution and storage.
Inventors:
SHIN HANG-CHEOL (KR)
OH JONG-KWANG (KR)
OH JONG-KWANG (KR)
Application Number:
PCT/KR2015/007734
Publication Date:
December 08, 2016
Filing Date:
July 24, 2015
Export Citation:
Assignee:
PNP BIOPHARM CO LTD (KR)
International Classes:
C07K14/50; A61K8/64; A61K38/18; C07K1/18
Foreign References:
| US20030008820A1 | 2003-01-09 | |||
| KR20150056024A | 2015-05-22 |
Other References:
OH, JONG KWANG: "Protein Engineering and Molecular Design of Human Fibloblast Growth Factor (FGF-2) for Higher Stability", MASTER'S THESIS, August 2014 (2014-08-01), pages i - ix, 1-26
GASPARIAN ET AL.: "Overexpression in Escherichia Coli and Purification of Human Fibroblast Growth Factor (FGF-2)", BIOCHEMISTRY (MOSCOW), vol. 74, no. 2, 2009, pages 221 - 225, XP055332474
DATABASE NCBI [O] 29 November 2004 (2004-11-29), XP055332476, Database accession no. AY820133.1
GASPARIAN ET AL.: "Overexpression in Escherichia Coli and Purification of Human Fibroblast Growth Factor (FGF-2)", BIOCHEMISTRY (MOSCOW), vol. 74, no. 2, 2009, pages 221 - 225, XP055332474
DATABASE NCBI [O] 29 November 2004 (2004-11-29), XP055332476, Database accession no. AY820133.1
Attorney, Agent or Firm:
Hanyang Patent Firm (KR)
한양특허법인 (KR)
한양특허법인 (KR)
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