Title:
HUMAN NON-ALCOHOLIC STEATOHEPATITIS MODEL
Document Type and Number:
WIPO Patent Application WO/2020/226070
Kind Code:
A1
Abstract:
A human non-alcoholic steatohepatitis model comprising an animal produced by raising a chimeric rodent animal, in which some or all of hepatocytes have been substituted by human hepatocytes, with a prepared feed having the properties (a), (b), and (c) mentioned below stably shows actual symptoms of human NASH: (a) The blending amount of fructose is 10% by weight or more relative to the whole amount of the prepared feed; (b) the content of fats is 10% by weight or more relative to the whole amount of the prepared feed, or the heat amount of the fats is 20 kcal% or more relative to the total heat amount (100 kcal%) of proteins, carbohydrates and the fats in the prepared feed; and (c) the blending amount of cholesterol is 1% by weight or more relative to the whole amount of the prepared feed.
More Like This:
Inventors:
KISOH KEISHI (JP)
MUKAIDANI CHISE (JP)
MUKAIDANI CHISE (JP)
Application Number:
PCT/JP2020/017578
Publication Date:
November 12, 2020
Filing Date:
April 23, 2020
Export Citation:
Assignee:
PHOENIXBIO CO LTD (JP)
International Classes:
A01K67/027; A23K20/105; A23K20/163; A23K50/50; C12N5/071; C12N15/09; G01N33/15; G01N33/50
Domestic Patent References:
| WO2008001614A1 | 2008-01-03 | |||
| WO2019088208A1 | 2019-05-09 |
Other References:
TSUCHIDA TAKUMA, LEE YOUNGMIN A., FUJIWARA NAOTO, YBANEZ MARIA, ALLEN BRITTANY, MARTINS SEBASTIAO, FIEL M. ISABEL, GOOSSENS NICOLA: "A simple diet- and chemical-induced murine NASH model with rapid progression of steatohepatitis'', fibrosis and liver cancer", J. HEPATOL., vol. 69, no. 2, 2018, pages 385 - 395, XP055760406
JENSEN VICTORIA SVOP, HVID HENNING, DAMGAARD JESPER, NYGAARD HELLE, INGVORSEN CAMILLA, WULFF ERIK MAX, LYKKESFELDT JENS, FLEDELIUS: "Dietary fat stimulates development of NAFLD more potently than dietary fructose in Sprague-Dawley rats", DIABETOL. METAB. SYNDR., vol. 10, no. 4, 2018, pages 1 - 13, XP055760409
ANSTEE, Q. M. ET AL.: "Mouse models in non- alcoholic fatty liver disease and steatohepatitis research", INT. J. EXP. PATH., vol. 87, 2006, pages 1 - 16, XP002552778, DOI: 10.1111/j.0959-9673.2006.00465.x
LONDON , R. M. ET AL.: "Pathogenesis of NASH: Animal Models", CLIN. LIVER DIS., vol. 11, 2007, pages 55 - 74
SANCHES SHEILA CRISTINA L., RAMALHO LEANDRA NAIRA Z., AUGUSTO MARLEI JOSIELE, DA SILVA DEISY MARA, RAMALHO FERNANDO SILVA: "Nonalcoholic steatohepatitis: A search for factual animal models", BIOMED RES. INT., vol. 2015, 2015, pages 1 - 13, XP055760414
JENSEN VICTORIA SVOP, HVID HENNING, DAMGAARD JESPER, NYGAARD HELLE, INGVORSEN CAMILLA, WULFF ERIK MAX, LYKKESFELDT JENS, FLEDELIUS: "Dietary fat stimulates development of NAFLD more potently than dietary fructose in Sprague-Dawley rats", DIABETOL. METAB. SYNDR., vol. 10, no. 4, 2018, pages 1 - 13, XP055760409
ANSTEE, Q. M. ET AL.: "Mouse models in non- alcoholic fatty liver disease and steatohepatitis research", INT. J. EXP. PATH., vol. 87, 2006, pages 1 - 16, XP002552778, DOI: 10.1111/j.0959-9673.2006.00465.x
LONDON , R. M. ET AL.: "Pathogenesis of NASH: Animal Models", CLIN. LIVER DIS., vol. 11, 2007, pages 55 - 74
SANCHES SHEILA CRISTINA L., RAMALHO LEANDRA NAIRA Z., AUGUSTO MARLEI JOSIELE, DA SILVA DEISY MARA, RAMALHO FERNANDO SILVA: "Nonalcoholic steatohepatitis: A search for factual animal models", BIOMED RES. INT., vol. 2015, 2015, pages 1 - 13, XP055760414
Attorney, Agent or Firm:
TADA Eiko et al. (JP)
Download PDF: