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Title:
HUMAN NOROVIRUS PROLIFERATION
Document Type and Number:
WIPO Patent Application WO/2019/189049
Kind Code:
A1
Abstract:
The present invention addresses the problem of establishing a method for proliferating human noroviruses (HuNoV) in vitro, the method avoiding the moral issues involved with conventional methods and the need to treat cells with bile. Specifically, the present invention relates to: a method for proliferating HuNoV, the method including a step for infecting an intestinal epithelial cell derived from a human iPS cell with HuNoV, and a step for culturing said intestinal epithelial cell; and a screening method for a HuNoV-proliferation inhibitor using said method.

Inventors:
SATO Shintaro (3-1 Yamadaoka, Suita-sh, Osaka 71, 〒5650871, JP)
YUKI Yoshikazu (3-1 Hongo 7-chome, Bunkyo-k, Tokyo 54, 〒1138654, JP)
KIYONO Hiroshi (3-1 Hongo 7-chome, Bunkyo-k, Tokyo 54, 〒1138654, JP)
Application Number:
JP2019/012649
Publication Date:
October 03, 2019
Filing Date:
March 26, 2019
Export Citation:
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Assignee:
THE UNIVERSITY OF TOKYO (3-1 Hongo 7-chome, Bunkyo-ku Tokyo, 54, 〒1138654, JP)
THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY (3-1 Yamadaoka, Suita-sh, Osaka 71, 〒5650871, JP)
International Classes:
C12N7/00; C12Q1/70; G01N33/15; G01N33/50
Domestic Patent References:
WO2017059449A12017-04-06
WO2017154795A12017-09-14
WO2016147975A12016-09-22
Other References:
ETTAYEBI, KHALIL ET AL.: "Replication of human noroviruses in stem cell -derived human enteroids", SCIENCE, vol. 353, no. 6306, 23 September 2016 (2016-09-23), pages 1387 - 1393, XP055471758, DOI: 10.1126/science.aaf5080
TREVISAN, MARTA ET AL.: "Modeling Viral Infectious Diseases and Development of Antiviral Therapies Using Human Induced Pluripotent Stem Cell -Derived Systems", VIRUSES, vol. 7, 13 July 2015 (2015-07-13), pages 3835 - 3856, XP055507471, DOI: 10.3390/v7072800
SPENCE, JASON R. ET AL.: "Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro", NATURE, vol. 470, no. 7332, 3 February 2011 (2011-02-03), pages 105 - 109, XP002743856
TAKAHASHI, YU ET AL.: "A Refined Culture System for Human Induced Pluripotent Stem Cell -Derived Intestinal Epithelial Organoids", STEM CELL REPORTS, vol. 10, no. 1, 9 January 2018 (2018-01-09), pages 314 - 328, XP055638955
SATO, SHINTARO ET AL.: "Human Norovirus Propagation in Human Induced Pluripotent Stem Cell -Derived Intestinal Epithelial Cells", CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY, vol. 7, no. 3, 10 December 2018 (2018-12-10), pages 686 - 688, XP055638957
Attorney, Agent or Firm:
OKUHARA Koji et al. (WinGreen Tachikawa branch, 305 Kangetsu Building 2-10-18, Shibasaki-cho, Tachikawa-sh, Tokyo 23, 〒1900023, JP)
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