Title:
HUMAN SIGNAL TRANSDUCTION SERINE/THREONINE PROTEIN KINASE
Document Type and Number:
WIPO Patent Application WO2000060062
Kind Code:
A3
Abstract:
An isolated and purified human signal transduction serine threonine protein kinase is described. A cDNA sequence which encodes the native signal transduction polypeptide is disclosed as well as the structural coding region and the amino acid residue sequence of the protein kinase. Methods are provided which employ the novel sequences to identify compounds that modulate the biological and/or pharmacological activity of the signal transduction protein kinase and hence regulate cellular and tissue physiology. Biologically-effective antisense molecules, as well as dominant negative mutant versions of the protein kinase, are described which are suitable for therapeutic use. The invention is also related to the diagnosis, study, prevention, and treatment of pathophysiological disorders related to or mediated by the signal transduction molecule.
Inventors:
NORRIS TYRRELL ERRICK
MOORE WILLIAM CRAIG
SILBERSTEIN DAVID SHAY
MOORE WILLIAM CRAIG
SILBERSTEIN DAVID SHAY
Application Number:
PCT/GB2000/001183
Publication Date:
January 18, 2001
Filing Date:
March 28, 2000
Export Citation:
Assignee:
ASTRAZENECA UK LTD (GB)
International Classes:
C12N9/12; C12N15/54; A61K48/00; (IPC1-7): C12N9/12; A61K48/00; C07K16/40; C12N5/10; C12N15/54; C12N15/63; C12Q1/48; C12Q1/68
Domestic Patent References:
| WO1999053036A2 | 1999-10-21 |
Other References:
HILLIER L. ET AL.: "The WashU-Merck EST Project; ym92f08.r1 Soares adult brain N2b4HB55Y Homo sapiens cDNA clone IMAGE:166407 5', mRNA sequence", EMBL DATABASE ENTRY HS460188; ACCESSION NO. R88460, 24 August 1995 (1995-08-24), XP002147780
HILLIER L. ET AL.: "The WashU-Merck EST Project; yf64a05.s1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE:26963 3', mRNA sequence", EMBL DATABASE ENTRY HS772102; ACCESSION NO. R37772, 4 May 1995 (1995-05-04), XP002147781
NAGASE T ET AL: "PREDICTION OF THE CODING SEQUENCES OF UNIDENTIFIED HUMAN GENES. XV. THE COMPLETE SEQUENCES OF 100 NEW CDNA CLONES FROM BRAIN WHICH CODE FOR LARGE PROTEINS IN VITRO", DNA RESEARCH, vol. 6, no. 5, 29 October 1999 (1999-10-29), pages 337 - 345, XP000865804, ISSN: 1340-2838
ABO A. ET AL.: "PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia", THE EMBO JOURNAL, vol. 17, no. 22, November 1998 (1998-11-01), pages 6527 - 6540, XP002147782
ROE B.A.: "Homo sapiens Chromosome 22q11.2 PAC Clone p201m18 In DGCR Region; complete sequence", EMBL DATABASE ENTRY HSAC97; ACCESSION NO. AC000097, 7 December 1996 (1996-12-07), XP002147852
WILLIAM S.: "Human DNA sequence from clone RP1-80N2 on chromosome 6p24.1-25.3. Contains the gene for MD-1, part of a putative novel gene, ESTs,STSs, GSSs and three putative GpG islands.", EMBL DATABASE ENTRY HS80N2; ACCESSION NO. AL031123, 24 July 1998 (1998-07-24), XP002147853
BAGRODIA S. ET AL.: "Pak to the future", TRENDS IN CELL BIOLOGY, vol. 9, no. 9, September 1999 (1999-09-01), pages 350 - 355, XP000925936
HILLIER L. ET AL.: "The WashU-Merck EST Project; yf64a05.s1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE:26963 3', mRNA sequence", EMBL DATABASE ENTRY HS772102; ACCESSION NO. R37772, 4 May 1995 (1995-05-04), XP002147781
NAGASE T ET AL: "PREDICTION OF THE CODING SEQUENCES OF UNIDENTIFIED HUMAN GENES. XV. THE COMPLETE SEQUENCES OF 100 NEW CDNA CLONES FROM BRAIN WHICH CODE FOR LARGE PROTEINS IN VITRO", DNA RESEARCH, vol. 6, no. 5, 29 October 1999 (1999-10-29), pages 337 - 345, XP000865804, ISSN: 1340-2838
ABO A. ET AL.: "PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia", THE EMBO JOURNAL, vol. 17, no. 22, November 1998 (1998-11-01), pages 6527 - 6540, XP002147782
ROE B.A.: "Homo sapiens Chromosome 22q11.2 PAC Clone p201m18 In DGCR Region; complete sequence", EMBL DATABASE ENTRY HSAC97; ACCESSION NO. AC000097, 7 December 1996 (1996-12-07), XP002147852
WILLIAM S.: "Human DNA sequence from clone RP1-80N2 on chromosome 6p24.1-25.3. Contains the gene for MD-1, part of a putative novel gene, ESTs,STSs, GSSs and three putative GpG islands.", EMBL DATABASE ENTRY HS80N2; ACCESSION NO. AL031123, 24 July 1998 (1998-07-24), XP002147853
BAGRODIA S. ET AL.: "Pak to the future", TRENDS IN CELL BIOLOGY, vol. 9, no. 9, September 1999 (1999-09-01), pages 350 - 355, XP000925936
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