Title:
HUMANIZED LACTATE DEHYDROGENASE (LDH) SACCHAROMYCES CEREVISIAE AND CONSTRUCTION METHOD THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/000870
Kind Code:
A1
Abstract:
Provided are a humanized lactate dehydrogenase (LDH) Saccharomyces cerevisiae and the construction method thereof. In particular, the method of gene homologous recombination is used to knockout the genes PDC1, PDC5 and PDC6 which encode the PDC of Saccharomyces cerevisiae, LDH from humans is expressed in Saccharomyces cerevisiae, and antimycin A is used to block electronic chain transfers, thus making humanized lactic acid fermentation replace ethanol fermentation for maintaining glycolysis. Humanized lactate dehydrogenase (LDH) Saccharomyces cerevisiae obtained from the present method can express the lactate dehydrogenase (LDH) of humans. LDHA or LDHC is expressed in strains of Saccharomyces cerevisiae suffering from three deficiencies in pdc1Δ pdc6Δ pdc5Δ. After antimycin A is added, the strains can grow, and the production of lactic acid can be detected.
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Inventors:
GAO XIAODONG (CN)
NAKANISHI HIDEKI (CN)
LI ZIJIE (CN)
NAKANISHI HIDEKI (CN)
LI ZIJIE (CN)
Application Number:
PCT/CN2016/087449
Publication Date:
January 05, 2017
Filing Date:
June 28, 2016
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/19; C12N9/04; C12N15/53; C12N15/81; C12R1/865
Foreign References:
| CN104911118A | 2015-09-16 | |||
| CN101886048A | 2010-11-17 |
Other References:
HIRASAWA, T. ET AL.: "Investigating the Effectiveness of DNA Microarray Analysis for Identifying the Genes Involved in L-Lactate Production by Saccharomyces Cerevisiae", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 84, no. 6, 2 September 2009 (2009-09-02), pages 1149 - 1159, XP019757485, ISSN: 1432-0614
LIAO, MINQI ET AL.: "Expression and Significance of Lactate Dehydrogenase-A in Human Cholangiocarcinoma Tissues and Cell Lines", ACTA UNIVERSITATIS MEDICINALIS NANJING, vol. 32, no. 5, 31 May 2012 (2012-05-31), pages 682 - 685, ISSN: 1007-4368
ZHAO, LIANGLIANG ET AL.: "Manipulation of Carbon Flux in Saccharomyces Cerevisiae to Improve L-Lactic Acid Production", ACTA MICROBIOLOGICA SINICA, vol. 51, no. 1, 4 January 2011 (2011-01-04), pages 50 - 58, ISSN: 0001-6209
LIAO, MINQI ET AL.: "Expression and Significance of Lactate Dehydrogenase-A in Human Cholangiocarcinoma Tissues and Cell Lines", ACTA UNIVERSITATIS MEDICINALIS NANJING, vol. 32, no. 5, 31 May 2012 (2012-05-31), pages 682 - 685, ISSN: 1007-4368
ZHAO, LIANGLIANG ET AL.: "Manipulation of Carbon Flux in Saccharomyces Cerevisiae to Improve L-Lactic Acid Production", ACTA MICROBIOLOGICA SINICA, vol. 51, no. 1, 4 January 2011 (2011-01-04), pages 50 - 58, ISSN: 0001-6209
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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