Title:
HYALURONIC ACID MICROSPHERES FOR SUSTAINED GENE TRANSFER
Document Type and Number:
WIPO Patent Application WO2000078358
Kind Code:
A3
Abstract:
A microsphere composition comprising materials which include substances which provide increased safety and bioavailability of nucleic acids when used in gene therapy applications. The microspheres of the present invention include hyaluronic acid which has been derivatized with a dihydrazide, preferably adipic dihydrazide, which is crosslinked to a nucleic acid. These microspheres are useful in gene therapy applications for the treatment of a variety of medical conditions, such as myocardial ischemia. In the treatment of myocardial ischemia, the microspheres of the invention include the VEGF gene. When cardiac cells are transfected with VEGF, angiogenesis in cardiac tissue is a result. Angiogenesis in cardiac tissue is likely to provide a therapeutic effect in the treatment of myocardial ischemia in that blocked or damaged blood vessels may be bypassed by newly grown blood vessels.
Inventors:
CHEN WEILIAM (US)
Application Number:
PCT/US2000/016837
Publication Date:
June 14, 2001
Filing Date:
June 19, 2000
Export Citation:
Assignee:
COLLABORATIVE GROUP LTD (US)
CHEN WEILIAM (US)
CHEN WEILIAM (US)
International Classes:
A61K9/16; A61K38/18; A61K38/19; A61K47/48; A61K48/00; (IPC1-7): C12N15/19; A61K9/16; A61K47/48; A61K48/00; A61P9/10
Domestic Patent References:
| WO1995015168A1 | 1995-06-08 | |||
| WO1994003269A1 | 1994-02-17 | |||
| WO1998032859A1 | 1998-07-30 |
Foreign References:
| EP0517565A2 | 1992-12-09 |
Other References:
ARRAS MARGARETE ET AL: "The delivery of angiogenic factors to the heart by microsphere therapy.", NATURE BIOTECHNOLOGY, vol. 16, no. 2, February 1998 (1998-02-01), pages 159 - 162, XP002155643, ISSN: 1087-0156
ILLUM L ET AL: "HYALURONIC ACID ESTER MICROSPHERES AS A NASAL DELIVERY SYSTEM FOR INSULIN", JOURNAL OF CONTROLLED RELEASE,NL,ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, vol. 29, no. 1/02, 1 February 1994 (1994-02-01), pages 133 - 141, XP000433658, ISSN: 0168-3659
SHERMAN A J ET AL: "Microsphere-based delivery of a replication-deficient adenoviral vector.", JOURNAL OF INVESTIGATIVE MEDICINE, vol. 45, no. 7, 1997, Midwestern Regional Meeting held jointly by the Central Society for Clinical Research, Midwestern Section American Federation for Medical Research, Midwest Society for Pediatric Research and Midwest R, pages 323A, XP000971442, ISSN: 1081-5589
ILLUM L ET AL: "HYALURONIC ACID ESTER MICROSPHERES AS A NASAL DELIVERY SYSTEM FOR INSULIN", JOURNAL OF CONTROLLED RELEASE,NL,ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, vol. 29, no. 1/02, 1 February 1994 (1994-02-01), pages 133 - 141, XP000433658, ISSN: 0168-3659
SHERMAN A J ET AL: "Microsphere-based delivery of a replication-deficient adenoviral vector.", JOURNAL OF INVESTIGATIVE MEDICINE, vol. 45, no. 7, 1997, Midwestern Regional Meeting held jointly by the Central Society for Clinical Research, Midwestern Section American Federation for Medical Research, Midwest Society for Pediatric Research and Midwest R, pages 323A, XP000971442, ISSN: 1081-5589
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