Title:
HYDROXYLASE GENE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2007/138894
Kind Code:
A1
Abstract:
A vitamin D3 hydroxylase is purified from a culturedPseudonocardia autotrophica cell, and a primer is designed based on the information on amino acid sequence for the hydroxylase. Subsequently, PCR is conducted using genomic DNA of Pseudonocardia autotrophica as a template to clone a gene for the vitamin D3 hydroxylase. By conducting a conversion reaction using a microorganism in which the gene is expressed using a proper expression system, a hydroxide of vitamin D or the like (e.g., hydroxy vitamin D3) can be produced with high efficiency.
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Inventors:
FUJII YOSHIKAZU (JP)
TAMURA TOMOHIRO (JP)
TAMURA TOMOHIRO (JP)
Application Number:
PCT/JP2007/060254
Publication Date:
December 06, 2007
Filing Date:
May 18, 2007
Export Citation:
Assignee:
MERCIAN CORP (JP)
NAT INST OF ADVANCED IND SCIEN (JP)
FUJII YOSHIKAZU (JP)
TAMURA TOMOHIRO (JP)
NAT INST OF ADVANCED IND SCIEN (JP)
FUJII YOSHIKAZU (JP)
TAMURA TOMOHIRO (JP)
International Classes:
C12N15/09; C12N1/21; C12N9/04; C12P7/02; C12P33/06
Foreign References:
| JP4064678B2 | 2008-03-19 | |||
| JPH02231089A | 1990-09-13 | |||
| JP2003325175A | 2003-11-18 | |||
| JPH02469A | 1990-01-05 | |||
| JPH02231089A | 1990-09-13 |
Other References:
SAWADA N. ET AL.: "Conversion of vitamin D3 to 1alpha,25-dihydroxyvitamin D3 by Streptomyces griseolus cytochrome P450SU-1", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 320, 2004, pages 156 - 164, XP004519078
SASAKI J. ET AL.: "Transformation of vitamin D3 to 1alpha,25-dihydroxyvitamin D3 via 25-hydroxyl vitamin D3 using Amycolata sp. strains", APPL. MICROBIOL. BIOTECHNOL., vol. 38, no. 2, 1992, pages 152 - 157, XP003019722
TAKEDA K. ET AL.: "Application of cyclodextrin to microbial transformation of vitamin D3 to 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3", J. FERMENT. BIOENG., vol. 78, 1994, pages 380 - 382, XP001053545
KAWAUCHI H ET AL., BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1219, 1994, pages 179 - 183
SAWADA ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 320, 2004, pages 156 - 164
UCHIDA ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 320, 2004, pages 156 - 164
SASAKI J. ET AL.: "Transformation of vitamin D3 to 1alpha,25-dihydroxyvitamin D3 via 25-hydroxyl vitamin D3 using Amycolata sp. strains", APPL. MICROBIOL. BIOTECHNOL., vol. 38, no. 2, 1992, pages 152 - 157, XP003019722
TAKEDA K. ET AL.: "Application of cyclodextrin to microbial transformation of vitamin D3 to 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3", J. FERMENT. BIOENG., vol. 78, 1994, pages 380 - 382, XP001053545
KAWAUCHI H ET AL., BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1219, 1994, pages 179 - 183
SAWADA ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 320, 2004, pages 156 - 164
UCHIDA ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 320, 2004, pages 156 - 164
Attorney, Agent or Firm:
OHIE, Kunihisa et al. (Selva-Ningyocho 6F 14-6, Nihonbashi-Ningyocho 2-chom, Chuo-ku Tokyo 13, JP)
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