Title:
HYDROXYNITRILE LYASE
Document Type and Number:
WIPO Patent Application WO/2017/150560
Kind Code:
A1
Abstract:
Provided are a method for obtaining an HNL gene and HNL derived from a millipede other than Chamberlinius hualienensis, and preparing a practically useable amount of HNL; and a method for producing optically active cyanohydrin using this HNL. A method for producing a millipede-derived HNL gene. A method that includes the selection of a gene having a base sequence that encodes a conserved amino acid sequence TAX1DIX2G (SEQ ID NO: 15) or VPNGDKIH (SEQ ID NO: 16) of millipede-derived HNL from genes present in an organism belonging to the Diplopoda. A protein having an amino acid sequence of any of (1)-(3) and having HNL activity. (1) An amino acid sequence listed in any of SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13, 83, 85, 87, or 89; (2) an amino acid sequence having amino acids deleted, substituted, and/or added in an amino acid sequence of (1); or (3) an amino acid sequence having 90% or greater identity to an amino acid sequence of (1). A method for preparing optically active cyanohydrin by causing this millipede-derived HNL to act on a reaction solvent that contains an aldehyde or the like and a substance that generates hydrogen cyanide or the like.
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Inventors:
ASANO YASUHISA (JP)
YAMAGUCHI TAKUYA (JP)
YAMAGUCHI TAKUYA (JP)
Application Number:
PCT/JP2017/007910
Publication Date:
September 08, 2017
Filing Date:
February 28, 2017
Export Citation:
Assignee:
UNIV TOYAMA PREFECTURAL (JP)
International Classes:
C12N15/09; C12N1/21; C12N5/07; C12N9/88; C12P13/00
Domestic Patent References:
| WO2006041226A1 | 2006-04-20 |
Foreign References:
| JP2000217590A | 2000-08-08 | |||
| JP2000217590A | 2000-08-08 | |||
| JP2015167477A | 2015-09-28 |
Other References:
DADASHIPOUR, M. ET AL.: "Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede, Chamberlinius hualienensis", PNAS, vol. 112, no. 34, 2015, pages 10605 - 10610, XP002770798
DUFFEY, S. S. ET AL.: "On the biochemical basis of HCN production in the millipede Harpaphe haydeniana (Xystodesmidae: Polydesmida", CANADIAN JOURNAL OF ZOOLOGY, vol. 56, no. 1, 1978, pages 7 - 16, XP008184247
LOBSTEIN, J. ET AL.: "SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm", MICROBIAL CELL FACTORIES, vol. 11, no. 56, 2012, pages 1 - 16, XP021129014
TAKUYA YAMAGUCHI ET AL.: "Molecular cloning of hydroxynitrile lyase from millipedes and heterologous expression in Escherichia coli", PROCEEDINGS OF THE 2016 ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 5 March 2016 (2016-03-05), pages 1039, XP009512440, ISSN: 2186-7976
NUYLERT, A. ET AL.: "3D049 Purification and characterization of hydroxynitrile lyase from millipedes, Parafontaria laminata", PROCEEDINGS OF THE 2016 ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 5 March 2016 (2016-03-05), pages 1038, XP009512441, ISSN: 2186-7976
DADASHIPUR ET AL., PROC. NATL. ACAD. SCI. USA., vol. 112, 2015, pages 10605 - 10610
NATURE METHODS, vol. 8, 2011, pages 785 - 786
BIOTECHNOL. PROG., vol. 20, 2004, pages 354 - 360
DUFFEY, S. S. ET AL.: "On the biochemical basis of HCN production in the millipede Harpaphe haydeniana (Xystodesmidae: Polydesmida", CANADIAN JOURNAL OF ZOOLOGY, vol. 56, no. 1, 1978, pages 7 - 16, XP008184247
LOBSTEIN, J. ET AL.: "SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm", MICROBIAL CELL FACTORIES, vol. 11, no. 56, 2012, pages 1 - 16, XP021129014
TAKUYA YAMAGUCHI ET AL.: "Molecular cloning of hydroxynitrile lyase from millipedes and heterologous expression in Escherichia coli", PROCEEDINGS OF THE 2016 ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 5 March 2016 (2016-03-05), pages 1039, XP009512440, ISSN: 2186-7976
NUYLERT, A. ET AL.: "3D049 Purification and characterization of hydroxynitrile lyase from millipedes, Parafontaria laminata", PROCEEDINGS OF THE 2016 ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 5 March 2016 (2016-03-05), pages 1038, XP009512441, ISSN: 2186-7976
DADASHIPUR ET AL., PROC. NATL. ACAD. SCI. USA., vol. 112, 2015, pages 10605 - 10610
NATURE METHODS, vol. 8, 2011, pages 785 - 786
BIOTECHNOL. PROG., vol. 20, 2004, pages 354 - 360
Attorney, Agent or Firm:
SIKS & CO. (JP)
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