Title:
IDENTIFICATION OF SOURCE OF DNA SAMPLES
Document Type and Number:
WIPO Patent Application WO/2011/101728
Kind Code:
A3
Abstract:
The present disclosure relates to methodology for fast and cost-effective identification of the source of DNA samples. DNA samples obtained from unknown or unrecognized tissues or cell types are analyzed according to the methodology described herein, yielding an identification of the tissue and/or cell type source. Identification is based on sequential biochemical procedures including methylation sensitive/dependent restriction and polymerase chain reaction, followed by analysis of the data. All biochemical steps are performed in a single test tube. The disclosure has immediate applications in forensic science for identification of the tissue source of DNA obtained from biological stains. The disclosure also has immediate applications in cancer diagnosis for identification.
Inventors:
WASSERSTROM ADAM (IL)
FRUMKIN DAN (IL)
FRUMKIN DAN (IL)
Application Number:
PCT/IB2011/000300
Publication Date:
March 01, 2012
Filing Date:
February 17, 2011
Export Citation:
Assignee:
NUCLEIX (IL)
WASSERSTROM ADAM (IL)
FRUMKIN DAN (IL)
WASSERSTROM ADAM (IL)
FRUMKIN DAN (IL)
International Classes:
C12Q1/68
Foreign References:
| US20090305234A1 | 2009-12-10 |
Other References:
SAKAMOTO ET AL.: "Cell type-specific methylation profiles occurring disproportionately in CpG- less regions that delineate developmental similarity", GENES CELLS, vol. 12, no. 10, October 2007 (2007-10-01), pages 1123 - 1132
VON KANEL ET AL.: "Quantitative one-step DNA methylation analysis (qOSMA) using native genomic DNA as template [online] Advances", GENOMICS, 28 January 2010 (2010-01-28), Retrieved from the Internet [retrieved on 20111122]
HOLEMON ET AL.: "MethylScreen: DNA methylation density monitoring using quantitative PCR", BIOTECHNIQUES, vol. 43, no. 5, November 2007 (2007-11-01), pages 683 - 693, XP002667851
EADS ET AL.: "MethyLight: a high-throughput assay to measure DNA methylation", NUCL ACIDS RES, vol. 28, no. 8, 15 April 2000 (2000-04-15), pages 1 - 8
GREINER ET AL.: "Effectiveness of capillary electrophoresis using fluorescent-labeled primers in detecting T-cell receptor gamma gene rearrangements", J MOL DESIGN, vol. 4, no. 3, August 2002 (2002-08-01), pages 137 - 143
VON KANEL ET AL.: "Quantitative one-step DNA methylation analysis (qOSMA) using native genomic DNA as template [online] Advances", GENOMICS, 28 January 2010 (2010-01-28), Retrieved from the Internet
HOLEMON ET AL.: "MethylScreen: DNA methylation density monitoring using quantitative PCR", BIOTECHNIQUES, vol. 43, no. 5, November 2007 (2007-11-01), pages 683 - 693, XP002667851
EADS ET AL.: "MethyLight: a high-throughput assay to measure DNA methylation", NUCL ACIDS RES, vol. 28, no. 8, 15 April 2000 (2000-04-15), pages 1 - 8
GREINER ET AL.: "Effectiveness of capillary electrophoresis using fluorescent-labeled primers in detecting T-cell receptor gamma gene rearrangements", J MOL DESIGN, vol. 4, no. 3, August 2002 (2002-08-01), pages 137 - 143
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