Title:
IMMOBILIZATION OF BIOCATALYSTS BY TEMPLATE-DIRECTED SILICATE PRECIPITATION
Document Type and Number:
WIPO Patent Application WO2005056808
Kind Code:
A3
Abstract:
This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, d-hydroxylysine, and d-hydroxy-w-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.
Inventors:
BOND RISHA (US)
JEWETT MICHAEL C (DK)
MCAULIFFE JOSEPH C (US)
WARD II DONALD E (US)
JEWETT MICHAEL C (DK)
MCAULIFFE JOSEPH C (US)
WARD II DONALD E (US)
Application Number:
PCT/US2004/041682
Publication Date:
November 09, 2006
Filing Date:
December 08, 2004
Export Citation:
Assignee:
GENENCOR INT (US)
DOW CORNING (US)
BOND RISHA (US)
JEWETT MICHAEL C (DK)
MCAULIFFE JOSEPH C (US)
WARD II DONALD E (US)
DOW CORNING (US)
BOND RISHA (US)
JEWETT MICHAEL C (DK)
MCAULIFFE JOSEPH C (US)
WARD II DONALD E (US)
International Classes:
C12N11/14; C07K17/04; C07K17/14; C11D3/386; C12N9/54; C12N11/04; C12N11/08; G01N33/544; G01N33/552; C12P
Foreign References:
| US6395299B1 | 2002-05-28 |
Other References:
LINDQUIST ET AL.: "The Role of Polyelectrolyte Charge Density and Molecular Weight on the Adsorption and Flocculation of Colloidal Silica with Polyethylenimine", JOURNAL OF COLLOID AND INTERFACE SCIENCE, vol. 55, no. 1, April 1976 (1976-04-01), pages 45 - 59, XP003002126
MIZUTANI ET AL.: "Silicic Acid Polymerization Catalyzed by Amines and polyamines", BULL. CHEM. SOC. JPN., vol. 71, 1998, pages 2017 - 2022, XP003002127
PATWARDHAN ET AL.: "Effect of Process Parameters on the Polymer Mediated Synthesis of Silica at Neutral pH", SILICON CHEMISTRY, vol. 1, 2002, pages 47 - 55, XP002351867
KROGER ET AL.: "Silica-Precipitating Peptides from Diatoms", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 28, 13 July 2001 (2001-07-13), pages 26066 - 26070, XP003002128
CORADIN ET AL.: "Interactions of Amino-Containing Peptides with Sodium Silicate and Colloidal Silica: A Biomimetic Approach to Silicification", LANGMUIR, vol. 18, 2002, pages 2331 - 2336, XP003002129
GILL I.: "Bio-Doped Nanocomposite Polymers: Sol-Gel Bioencapsulates", CHEM. MATER., vol. 13, 2001, pages 3404 - 3421, XP002291608
MIZUTANI ET AL.: "Silicic Acid Polymerization Catalyzed by Amines and polyamines", BULL. CHEM. SOC. JPN., vol. 71, 1998, pages 2017 - 2022, XP003002127
PATWARDHAN ET AL.: "Effect of Process Parameters on the Polymer Mediated Synthesis of Silica at Neutral pH", SILICON CHEMISTRY, vol. 1, 2002, pages 47 - 55, XP002351867
KROGER ET AL.: "Silica-Precipitating Peptides from Diatoms", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 28, 13 July 2001 (2001-07-13), pages 26066 - 26070, XP003002128
CORADIN ET AL.: "Interactions of Amino-Containing Peptides with Sodium Silicate and Colloidal Silica: A Biomimetic Approach to Silicification", LANGMUIR, vol. 18, 2002, pages 2331 - 2336, XP003002129
GILL I.: "Bio-Doped Nanocomposite Polymers: Sol-Gel Bioencapsulates", CHEM. MATER., vol. 13, 2001, pages 3404 - 3421, XP002291608
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