IMMOBILIZED SUBSTRATE, SEPARATION GELS AND METHOD FOR DETECTING ENZYMATIC ACTIVITY

Title:

IMMOBILIZED SUBSTRATE, SEPARATION GELS AND METHOD FOR DETECTING ENZYMATIC ACTIVITY

Document Type and Number:

WIPO Patent Application WO1999054729

Kind Code:

A3

Abstract:

The invention relates to a method for fixing substrate molecules in gel matrices. Said method is characterized in that a substrate is covalently linked to a monomeric or oligomeric structural unit of a gel and the resulting product is polymerized. The invention also relates to gels produced using said method.

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Inventors:

FRANK RONALD (DE)
TEGGE WERNER (DE)

Application Number:

PCT/EP1999/002688

Publication Date:

February 17, 2000

Filing Date:

April 21, 1999

Export Citation:

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Assignee:

BIOTECHNOLOG FORSCHUNG GMBH (DE)
FRANK RONALD (DE)
TEGGE WERNER (DE)

International Classes:

G01N27/447; C07K17/06; C12Q1/00; C12Q1/48; G01N21/64; G01N33/483; G01N33/52; G01N33/53; G01N33/543; G01N33/561; G01N27/62; G01N30/90; C12Q; (IPC1-7): C12Q1/00; C07K17/06; G01N30/48; G01N33/543

Domestic Patent References:

WO1999008717A2	1999-02-25
WO1993009176A2	1993-05-13
WO1992013447A1	1992-08-20
WO1991008242A1	1991-06-13
WO1995015983A1	1995-06-15

Foreign References:

US5405766A

1995-04-11

Other References:

I. KAMESHITA ET AL.: "Detection of protein kinase activities toward oligopeptides in sodium dodecyl sulfate-polyacrylamide gel.", ANALYTICAL BIOCHEMISTRY., vol. 237, 1996, ACADEMIC PRESS INC. NEW YORK., US, pages 198 - 203, XP002123331, ISSN: 0003-2697

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