Title:
IMMORTALIZED PIG-FETUS SMALL-INTESTINAL MACROPHAGES
Document Type and Number:
WIPO Patent Application WO/2022/107793
Kind Code:
A1
Abstract:
Attempts have been made to isolate macrophages from the small intestine of approximately one-month-old pigs and immortalize the macrophages, but microbial contamination was severe and isolation of macrophages from the porcine small intestine was not successful even when medium containing antibiotics and antifungals was used. However, it was discovered that the contamination is suppressed and primary culture of macrophages is possible if the small intestine of a pig fetus is used. Furthermore, it was clarified that the small-intestinal macrophages of the primary culture can be immortalized by causing SV40 large T antigen and telomerase reverse transcriptase to be expressed, and it became possible to provide a cell line of subculturable porcine small-intestinal macrophages.
Inventors:
TAKENOUCHI TAKATO (JP)
SUZUKI SHUNICHI (JP)
UENISHI HIROHIDE (JP)
MIYAZAKI AYAKO (JP)
MASUJIN KENTARO (JP)
KOKUHO TAKEHIRO (JP)
TAKAGI MICHIHIRO (JP)
SUZUKI SHUNICHI (JP)
UENISHI HIROHIDE (JP)
MIYAZAKI AYAKO (JP)
MASUJIN KENTARO (JP)
KOKUHO TAKEHIRO (JP)
TAKAGI MICHIHIRO (JP)
Application Number:
PCT/JP2021/042171
Publication Date:
May 27, 2022
Filing Date:
November 17, 2021
Export Citation:
Assignee:
NAT AGRICULTURE & FOOD RES ORG (JP)
International Classes:
C12N5/10; C12N7/01; C12N15/86; C12Q1/06; C12Q1/6897
Foreign References:
| JP2019520827A | 2019-07-25 |
Other References:
TAKENOUCHI, TAKATO; KITANI, HIROSHI; SUZUKI, SHUNICHI; NAKAI, MICHIKO; FUCHIMOTO, DAIICHIRO; TSUKIMOTO, MITSUTOSHI; SHINKAI, HIROK: "2A24a02 Establishment and characteristic analysis of porcine macrophage immortalized cell lines", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 2018 (JSBBA 2018), vol. 2018, 5 March 2018 (2018-03-05), pages 768, XP009536796
TAKENOUCHI TAKATO, KITANI HIROSHI, SUZUKI SHUNICHI, NAKAI MICHIKO, FUCHIMOTO DAI-ICHIRO, TSUKIMOTO MITSUTOSHI, SHINKAI HIROKI, SAT: "Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase", FRONTIERS IN VETERINARY SCIENCE, vol. 4, XP055815837, DOI: 10.3389/fvets.2017.00132
PARK JUNG-EUN; SHIN HYUN-JIN: "Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages", VIRUS RESEARCH, AMSTERDAM, NL, vol. 191, 10 August 2014 (2014-08-10), NL , pages 143 - 152, XP029060936, ISSN: 0168-1702, DOI: 10.1016/j.virusres.2014.07.038
TAKENOUCHI TAKATO, KITANI HIROSHI, SUZUKI SHUNICHI, NAKAI MICHIKO, FUCHIMOTO DAI-ICHIRO, TSUKIMOTO MITSUTOSHI, SHINKAI HIROKI, SAT: "Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase", FRONTIERS IN VETERINARY SCIENCE, vol. 4, XP055815837, DOI: 10.3389/fvets.2017.00132
PARK JUNG-EUN; SHIN HYUN-JIN: "Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages", VIRUS RESEARCH, AMSTERDAM, NL, vol. 191, 10 August 2014 (2014-08-10), NL , pages 143 - 152, XP029060936, ISSN: 0168-1702, DOI: 10.1016/j.virusres.2014.07.038
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
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