Title:
IMMUNE CELL TREATED BY MEANS OF MAGNETIC FIELD AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/219119
Kind Code:
A1
Abstract:
Provided are an immune cell treated by means of a magnetic field and the use thereof. A medium static magnetic field with an intensity of 0.3T can promote the secretion of granzymes and cytokines of mouse CD8+ T cells, increase the level of ATP and mitochondrial respiration, and up-regulate the expression of mitochondrial respiratory chain related genes Uqcrb and/or Ndufs6. In addition, magnetic receptor candidate genes Isca1 and Cry1/Cry2 are involved in regulating the expression of Uqcrb and/or Ndufs6. An in-vivo experiment shows that the static magnetic field can inhibit the growth and development of tumors by means of promoting the secretion of the granzymes and cytokines of the CD8+ T cells. A killing ability test shows that the static magnetic field treatment can enhance the killing ability of the CD8+ T cells, and infusion of the CD8+ T cells treated by means of the magnetic field back into a tumor-grafted mouse has an anti-tumor effect.
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Inventors:
ZHU XIAOYAN (CN)
FANG ZHICAI (CN)
FANG YANWEN (CN)
LIU YAN (CN)
CAO XIANXIA (CN)
LIU HAIFENG (CN)
FANG ZHICAI (CN)
FANG YANWEN (CN)
LIU YAN (CN)
CAO XIANXIA (CN)
LIU HAIFENG (CN)
Application Number:
PCT/CN2021/091341
Publication Date:
November 04, 2021
Filing Date:
April 30, 2021
Export Citation:
Assignee:
HEYE HEALTH TECH CO LTD (CN)
CENTER FOR EXCELLENCE IN MOLECULAR CELL SCIENCE CHINESE ACAD OF SCIENCES (CN)
CENTER FOR EXCELLENCE IN MOLECULAR CELL SCIENCE CHINESE ACAD OF SCIENCES (CN)
International Classes:
C12N13/00; A61K35/15; A61K35/17; A61P35/00; A61P35/02; C12N5/0781; C12N5/0783; C12N5/0784; C12N5/0786; C12N5/0787
Domestic Patent References:
| WO2019221991A1 | 2019-11-21 |
Foreign References:
| CN111733154A | 2020-10-02 |
Other References:
ZHU XIAOYAN, LIU YAN, CAO XIANXIA, LIU HAIFENG, SUN AO, SHEN HAO, ZHAO JINGYAO, LI RONGHONG, WU LIGANG, FANG ZHICAI, WANG HUI, ZHA: "Moderate static magnetic fields enhance antitumor CD8+ T cell function by promoting mitochondrial respiration", SCIENTIFIC REPORTS, vol. 10, no. 1, 1 December 2020 (2020-12-01), XP055862706, DOI: 10.1038/s41598-020-71566-x
ZHU XIAOYAN, ZHICAI FANG, LIJIANG HU, YANWEN FNAG, MENGYU LIU: "Biological effects and related mechanisms of magnetic fields on immune cells", CHEMISTRY OF LIFE, vol. 39, no. 5, 12 October 2019 (2019-10-12), pages 875 - 884, XP055862775, DOI: 10.13488/j.smhx.20190252
YAP JASMINE LYE YEE, TAI YEE KIT, FRÖHLICH JÜRG, FONG CHARLENE HUI HUA, YIN JOCELYN NAIXIN, FOO ZI LING, RAMANAN SHARANYA, BEYER C: "Ambient and supplemental magnetic fields promote myogenesis via a TRPC1‐mitochondrial axis: evidence of a magnetic mitohormetic mechanism", THE FASEB JOURNAL, FEDERATION OF AMERICAN SOCIETIES FOR EXPERIMENTAL BIOLOGY, & EXPERIMENTAL BIOLOGY MEETING; SAN DIEGO, CA, USA; APRIL 21 -25, 2018, vol. 33, no. 11, 1 November 2019 (2019-11-01), & EXPERIMENTAL BIOLOGY MEETING; SAN DIEGO, CA, USA; APRIL 21 -25, 2018 , pages 12853 - 12872, XP055862714, ISSN: 0892-6638, DOI: 10.1096/fj.201900057R
GHODBANE SOUMAYA, LAHBIB AIDA, SAKLY MOHSEN, ABDELMELEK HAFEDH: "Bioeffects of Static Magnetic Fields: Oxidative Stress, Genotoxic Effects, and Cancer Studies", BIOMED RESEARCH INTERNATIONAL, HINDAWI PUBLISHING CORPORATION, vol. 2013, 1 January 2013 (2013-01-01), pages 1 - 12, XP055862717, ISSN: 2314-6133, DOI: 10.1155/2013/602987
YANG JZHANG JDING CDONG DSHANG P: "Regulation of Osteoblast Differentiation and Iron Content in MC3T3-E1 Cells by Static Magnetic Field with Different Intensities", BIOLOGICAL TRACE ELEMENT RESEARCH, 2017
ZHANG LWANG JWANG HWANG WLI ZLIU J ET AL.: "Moderate and strong static magnetic fields directly affect EGFR kinase domain orientation to inhibit cancer cell proliferation", ONCOTARGET, vol. 7, no. 27, 2016, pages 41527 - 41539
PRASAD ATEH DBLBLASIAK ACHAI CWU YGHARIBANI PM ET AL.: "Static Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors", SCIENTIFIC REPORTS, vol. 7, no. 1, 2017, pages 6743
"NCBI", Database accession no. GSE113858
ZHU XIAOYAN, ZHICAI FANG, LIJIANG HU, YANWEN FNAG, MENGYU LIU: "Biological effects and related mechanisms of magnetic fields on immune cells", CHEMISTRY OF LIFE, vol. 39, no. 5, 12 October 2019 (2019-10-12), pages 875 - 884, XP055862775, DOI: 10.13488/j.smhx.20190252
YAP JASMINE LYE YEE, TAI YEE KIT, FRÖHLICH JÜRG, FONG CHARLENE HUI HUA, YIN JOCELYN NAIXIN, FOO ZI LING, RAMANAN SHARANYA, BEYER C: "Ambient and supplemental magnetic fields promote myogenesis via a TRPC1‐mitochondrial axis: evidence of a magnetic mitohormetic mechanism", THE FASEB JOURNAL, FEDERATION OF AMERICAN SOCIETIES FOR EXPERIMENTAL BIOLOGY, & EXPERIMENTAL BIOLOGY MEETING; SAN DIEGO, CA, USA; APRIL 21 -25, 2018, vol. 33, no. 11, 1 November 2019 (2019-11-01), & EXPERIMENTAL BIOLOGY MEETING; SAN DIEGO, CA, USA; APRIL 21 -25, 2018 , pages 12853 - 12872, XP055862714, ISSN: 0892-6638, DOI: 10.1096/fj.201900057R
GHODBANE SOUMAYA, LAHBIB AIDA, SAKLY MOHSEN, ABDELMELEK HAFEDH: "Bioeffects of Static Magnetic Fields: Oxidative Stress, Genotoxic Effects, and Cancer Studies", BIOMED RESEARCH INTERNATIONAL, HINDAWI PUBLISHING CORPORATION, vol. 2013, 1 January 2013 (2013-01-01), pages 1 - 12, XP055862717, ISSN: 2314-6133, DOI: 10.1155/2013/602987
YANG JZHANG JDING CDONG DSHANG P: "Regulation of Osteoblast Differentiation and Iron Content in MC3T3-E1 Cells by Static Magnetic Field with Different Intensities", BIOLOGICAL TRACE ELEMENT RESEARCH, 2017
ZHANG LWANG JWANG HWANG WLI ZLIU J ET AL.: "Moderate and strong static magnetic fields directly affect EGFR kinase domain orientation to inhibit cancer cell proliferation", ONCOTARGET, vol. 7, no. 27, 2016, pages 41527 - 41539
PRASAD ATEH DBLBLASIAK ACHAI CWU YGHARIBANI PM ET AL.: "Static Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors", SCIENTIFIC REPORTS, vol. 7, no. 1, 2017, pages 6743
"NCBI", Database accession no. GSE113858
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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