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Title:
IMMUNE INDUCER COMPRISING ANTIGEN PEPTIDE-ADJUVANT NUCLEOTIDE CONJUGATE AND PHARMACEUTICAL COMPOSITION COMPRISING SAME
Document Type and Number:
WIPO Patent Application WO/2020/067400
Kind Code:
A1
Abstract:
Provided are: an immune inducer that comprises, as an active ingredient, a polynucleotide/peptide conjugate wherein a CpG motif-containing single-stranded polynucleotide or polynucleotide derivative is bonded to a peptide having antigenicity via a spacer, said spacer being covalently bonded to the polynucleotide or polynucleotide derivative at one terminus and also covalently bonded to the peptide having antigenicity at the other terminus; and a pharmaceutical composition comprising the same.

Inventors:
MOCHIZUKI SHINICHI (JP)
KOIZUMI MAKOTO (JP)
MORITA KOJI (JP)
Application Number:
PCT/JP2019/038090
Publication Date:
April 02, 2020
Filing Date:
September 27, 2019
Export Citation:
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Assignee:
THE UNIV OF KITAKYUSHU (JP)
DAIICHI SANKYO CO LTD (JP)
International Classes:
A61K39/39; A61K47/61; A61P35/00; A61P37/04; C07K4/00; C07K14/00
Domestic Patent References:
WO2016152767A12016-09-29
WO2001034207A12001-05-17
WO2002072152A12002-09-19
WO2015118789A12015-08-13
Foreign References:
JP2017500313A2017-01-05
JP2010174107A2010-08-12
JP2007070307A2007-03-22
Other References:
SHIROTA HIDEKAZU, KLINMAN DENNIS M.: "Chapter 27: TLR-9 agonist immunostimulatory sequence adjuvants linked to cancer antigens", CANCER VACCINES - SERIES TITLE: METHODS IN MOLECULAR BIOLOGY., vol. 1139, 2014, pages 337 - 344, XP009527495, ISBN: 978-1-4939-0344-3, DOI: 10.1007/978-1-4939-0345-0_27
KRAMER, K. ET AL.: "Intracellular cleavable CpG oligodeoxynucleotide-antigen conjugate enhances anti-tumor immunity", MOLECULAR THERAPY, vol. 25, no. 1, 2017, pages 62 - 70, XP055613007, DOI: 10.1016/j.ymthe.2016.10.001
KRAMER, K. ET AL.: "Comparative study of 5'-and 3'- linked CpG-antigen conjugates for the induction of cellular immune responses", ACS OMEGA, vol. 2, 2017, pages 227 - 235, XP055617506, DOI: 10.1021/acsomega.6b00368
KUPIHAR, Z. ET AL.: "Synthesis and application of a novel, crystalline phosphoramidite monomer with thiol terminus, suitable for the synthesis of DNA conjugates", BIOORGANIC & MEDICINAL CHEMISTRY, vol. 9, 2001, pages 1241 - 1247, XP055045403, DOI: 10.1016/S0968-0896(00)00339-4
BACTERIAL CPG DNA ACTIVATES IMMUNE CELLS TO SIGNAL INFECTIOUS DANGERH. WAGNER, ADV. IMMUNOL., vol. 73, 1999, pages 329 - 368
M. KRIEG: "CpG Motifs in Bacterial DNA and Their Immune Effects", ANNU. REV. IMMUNOL., vol. 20, 2002, pages 709 - 760, XP009016455, DOI: 10.1146/annurev.immunol.20.100301.064842
S. YAMAMOTOT. YAMAMOTOT. TOKUNAGA: "The Discovery of Immunostimulatory DNA Sequence", SPRINGER SEMINARS IN IMMUNOPATHOLOGY, vol. 22, 2000, pages 11 - 19
"Standard Immunology", vol. 333, 2002, IGAKU-SHOIN LTD.
K. SAKURAIS. SHINKAI: "Molecular Recognition of Adenine, Cytosine, and Uracil in a Single-Stranded RNA by a Natural Polysaccharide: Schizophyllan", J. AM. CHEM. SOC., vol. 122, 2000, pages 4520 - 4521, XP002936530, DOI: 10.1021/ja0000145
K. SAKURAIM. MIZUS. SHINKAI: "Polysaccharide-Polynucleotide Complexes. 2. Complementary Polynucleotide Mimic Behavior of the Natural Polysaccharide Schizophyllan in the Macromolecular Complex with Single-Stranded RNA and DNA", BIOMACROMOLECULES, vol. 2, 2001, pages 641 - 650, XP002504242, DOI: 10.1021/BM000121R
S. MOCHIZUKIK. SAKURAI: "Dectin-1 Targeting Delivery of TNF-a Antisense ODNs Complexed with P-1,3-glucan Protects Mice from LPS-induced Hepatitis", J. CONTROL. RELEASE, vol. 151, 2011, pages 155 - 161, XP055197019, DOI: 10.1016/j.jconrel.2011.01.026
K. MIYOSHIK. UEZUK. SAKURAIS. SHINKAI: "Structural Analysis of the Curdlan/Poly(cytidylic acid) Complex with Semiempirical Molecular Orbital Calculations", BIOMACROMOLECULES, vol. 6, 2005, pages 1540 - 1546
M. MIZUK. KOUMOTOT. ANADAT. MATSUMOTOM. NUMATAS. SHINKAIT. NAGASAKIK. SAKURAI: "A Polysaccharide Carrier for Immunostimulatory CpG DNAs to Enhance Cytokine Secretion", J. AM. CHEM. SOC., vol. 126, 2004, pages 8372 - 8373, XP055146435, DOI: 10.1021/ja031978+
M. MIZUK. KOUMOTOT. KIMURAK. SAKURAIS. SHINKAI: "Protection of Polynucleotides against Nuclease-mediated Hydrolysis by Complexation with Schizophyllan", BIOMATERIALS, vol. 25, no. 15, 2004, pages 3109 - 3116, XP004489064, DOI: 10.1016/j.biomaterials.2003.09.079
N. SHIMADAK. J. ISHIIY. TAKEDAC. COBANY. TORIIS. SHINKAIS. AKIRAK. SAKURAI: "Synthesis and in Vitro Characterization of Antigen-Conjugated Polysaccharide as a CpG DNA Carrier", BIOCONJUGATE CHEM., vol. 17, 2006, pages 1136 - 1140
KHAN S. ET AL.: "Distinct Uptake Mechanisms but Similar Intracellular Processing of Two Different Toll-like Receptor Ligand-Peptide Conjugates in Dendritic Cells", J. BIOL. CHEM., vol. 282, 2007, pages 21145 - 21159, XP055613005, DOI: 10.1074/jbc.M701705200
KRAMER K ET AL.: "Intracellular Cleavable CpG Oligodeoxynucleotide-Antigen Conjugate Enhances Anti-tumor Immunity", MOL. THER., vol. 25, 2017, pages 62 - 70, XP055613007, DOI: 10.1016/j.ymthe.2016.10.001
CHOWELL ET AL.: "TCR contact residue hydrophobicity is a hallmark of immunogenic CD8+ T cell epitopes", PNAS, vol. 112, no. 14, 7 April 2015 (2015-04-07), pages E1754 - E1762
See also references of EP 3858383A4
Attorney, Agent or Firm:
ONO, Shinjiro et al. (JP)
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