Title:
IMMUNOCHROMATOGRAPHY TEST PIECE FOR EXTRACTING AND MEASURING SUGAR CHAIN ANTIGEN, AND IMMUNOCHROMATOGRAPHY METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2017/213228
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide an immunochromatography test piece that enables the specific measurement of a sugar chain antigen. The present invention is an immunochromatography test piece for measuring a sugar chain antigen, and includes a sample pad to which a specimen is added, a marker area including a marker antibody obtained by labeling an antibody with a sugar chain antigen, a detection area in which an antibody to the sugar chain antigen is immobilized, and in which an antibody-sugar chain antigen-marker antibody complex is formed in the detection area. The method for measuring a sugar chain antigen in a sample by immunochromatography uses an immunochromatography test piece having an area impregnated with a neutralizing agent upstream of the marker area, and has an area impregnated with tartaric acid further upstream than said area impregnated with neutralizing reagent. The method for measuring a sugar chain antigen in a specimen by immunochromatography includes mixing the specimen with a nitrite solution and adding to a sample pad of the immunochromatography test piece. In the area impregnated with tartaric acid, a sugar chain antigen is extracted from the specimen by the action of nitrous acid generated by the reaction between nitrite and tartaric acid, an acidic solution including the sugar chain antigen is neutralized in the area including a neutralizing reagent, and an antigen-sugar chain antigen-marker antibody is formed in the detection area.
Inventors:
KATO DAISUKE (JP)
HATTORI TOMOHIRO (JP)
MURAMATSU SHINO (JP)
HATTORI TOMOHIRO (JP)
MURAMATSU SHINO (JP)
Application Number:
PCT/JP2017/021340
Publication Date:
December 14, 2017
Filing Date:
June 08, 2017
Export Citation:
Assignee:
DENKA SEIKEN KK (JP)
International Classes:
G01N33/543; C07K14/005; C07K14/195; C07K14/29; C07K14/295; C07K14/30; C07K14/44; G01N33/569
Foreign References:
| JP2015034719A | 2015-02-19 | |||
| JP2014232064A | 2014-12-11 | |||
| JP2003215126A | 2003-07-30 | |||
| JP3166728U | 2011-03-24 | |||
| JP2008224543A | 2008-09-25 | |||
| US20050277163A1 | 2005-12-15 |
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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